Antimicrobial Property of Hydrocolloid Impression Material Incorporated with Silver Nanoparticles AgainstStaphylococcusAureus

Abstract

Abstract. Dental impressions can easily become contaminated with patient's blood and saliva which are capable of transmitting infectious diseases to dental personnel. The addition of antimicrobial agents into impression materials could be effective in reducing the chances of cross-infection. Silver nanoparticles have been applied in dentistry as a potent antimicrobial agent. This study aims to evaluate the in vitro antimicrobial efficacy of silver nanoparticles incorporated to irreversible hydrocolloid impression material against Staphylococcus aureus. Silver nanoparticles (AgZrPO4, National Direct Network Company, Thailand) at concentrations of 0.25%, 0.50%, 1.00% and 1.50% w/w were added to powder of impression materials (Kromopan, Lascod, Ilaty). Impression material samples were prepared on sterile plate in accordance with manufacturer's instruction. After setting, a 100 microliter of S. aureus ATCC6538 suspension (10 6 cells/mL) were inoculated on the surface of the impression sample and left for 10 minutes. The amount of S. aureus on the surface was quantified using imprint technique on Mannitol Salt agar. Impression materials incorporated with AgZrPO4 showed antimicrobial property against S. aureus (up to 95% reduction) compared with control (impression material without AgZrPO4). Even though the mechanism of antimicrobial action was not clearly understood, AgZrPO4 incorporated to impression material was demonstrated to possess an inhibitory effect against pathogenic bacteria. Further studies are needed to investigate physical properties of the material and the clinical usage.

Antimicrobial Properties of Ag Nanoparticle-Incorporated Irreversible Hydrocolloid Impression Material

“…The antibacterial property of hydrocolloid impression material containing Ag nanoparticles was revealed against S. aureus at concentrations as low as 0.25% w/w. The effect was dose dependent identical to the present study [14]. The results of this previous study, through statistical analysis, found no significant difference in the bacterial inhibition between the control group and the 0.25% AgZrPO 4 containing impression material.…”

Marginal fit of interim restoration constructed by 3D printing versus conventional chair-side method: An in vitro study

“…7 Additionally, the same software was used to standardize the occlusal thickness of all crowns, which was fixed to 1.5mm from the central fossa [14] . Measurement of marginal gap was done without cementation of the crowns on to epoxy dies, in order to exclude the effect of cementation technique variations on marginal gap values as reported by different authors [15,16] . Assessment of marginal gap values was done using a stereomicroscope, as it was reported to be an accurate technique of evaluation by different studies [10,17,18] .…”

Physical Properties of Irreversible Hydrocolloid Impression Material Incorporated with Silver-Nanoparticles

“…The small size of these particles makes penetration through cell membranes easier, thus affecting intracellular processes resulting in higher reactivity and antimicrobial activity; moreover, it showed low toxicity and good biocompatibility with human cells [17][18][19]. A recent study on AgNPs incorporated irreversible hydrocolloid impression material achieved similar antimicrobial activity against pathogenic microbes suggesting further investigations into the materials' physical properties [20]. This study therefore aims to evaluate the physical properties of irreversible hydrocolloid impression material containing AgNPs.…”