Antimicrobial properties of the novel bacterial isolate Paenibacilllus sp. SMB1 from a halo-alkaline lake in India

Abstract

Antibiotic-resistance is ever growing burden on our society for the past many years. Many synthetic chemistry approaches and rational drug-design have been unable to pace up and tackle this problem. Natural resources, more specifically, the microbial diversity, on the other hand, make a traditional and still the best platform to search for new chemical scaffolds and compounds. Here, we report the antimicrobial characteristics of novel bacterial isolate from a salt lake in India. We screened the bacterial isolates for their inhibitory activity against indicator bacteria and found that four novel species were able to prevent the growth of test strains studied in vitro . Further, we characterized one novel species (SMB1 T = SL4-2) using polyphasic taxonomic approaches and also purified the active ingredient from this bacterium. We successfully characterized the antimicrobial compound using mass spectroscopy and amino acid analysis. We also allocated two novel biosynthetic gene clusters for putative bacteriocins and one novel non-ribosomal peptide gene cluster in its whole genome. We concluded that the strain SMB1 T belonged to the genus Paenibacilllus with the pairwise sequence similarity of 98.67% with Paenibacillus tarimensis DSM 19409 T and we proposed the name Paenibacillus sambharensis sp. nov. The type strain is SMB1 T (=MTCC 12884 = KCTC 33895 T ).

Characterization of a Potent New-Generation Antimicrobial Peptide of Bacillus

“…The LCMS data was processed using proteome discoverer software against UniProt Bacillus database at MS1 and MS2 tolerance of 10 ppm and 0.5 Da, respectively ( Figures 2C,C1 ). The identified peptide is bacitracin as reported earlier ( Singh et al, 2019 ).…”

Antimicrobial activities of Bacillus velezensis strains isolated from stingless bee products against methicillin-resistant Staphylococcus aureus

“…Exploration of antimicrobial metabolites from natural sources such as bacteria is considered as one of the most promising alternatives to combat the infection of pathogens in humans, animals and plants [9,11,[36][37][38]. Based on the previous studies, the Bacillus subtilis complex group which is comprised of B. subtilis, B. amyloliquefaciens, B. licheniformis, B. velezensis and B. pumilus, is one of the most common groups of bacteria associated with antimicrobial metabolite production [10,39].…”

Paenibacillus alvei MP1 as a Producer of the Proteinaceous Compound with Activity against Important Human Pathogens, Including Staphylococcus aureus and Listeria monocytogenes

“…Recent studies show that, despite a negative opinion about Paenibacillus group members, mainly as a result of P. larvae being recognized as the causative agent of American Foulbrood, bacteria belonging to this genus are a rich source of metabolites with promising antimicrobial activity [25][26][27]. P. alvei AN5 [28] was detected as a producer of an antimicrobial compound against both Gram-positive and Gram-negative bacterial strains, including S. aureus and E. coli ATCC 29522.…”

“…Recent studies show that despite a negative opinion about Paenibacillus group members, mainly 215 as a result of P. larvae being recognized as the causative agent of American Foulbrood, bacteria 216 belonging to this genus are a rich source of metabolites with promising antimicrobial activity [25][26][27].…”