Antimicrobial properties of stem bark extracts from Phyllanthus muellerianus (Kuntze) Excell

Brusotti, Gloria; Cesari, I.; Frassà, G.; Grisoli, Pietro; Dacarro, Cesare; Caccialanza, Gabriele

doi:10.1016/j.jep.2011.03.042

Cited by 36 publications

(13 citation statements)

References 10 publications

(10 reference statements)

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“…The phytochemical screening showed almost the same chemical composition (tannins, flavonoids, polyphenols, saponins, alkaloids, and anthraquinones), except that alkaloids were absent in our study. Another study revealed the antitimicrobial properties of the methanolic extract of the stem bark of Phyllanthus muellerianus against Clostridium sporogenes , Streptococcus mutans and Streptococcus pyogenes [ 39 ]. P. muellerianus presented no adverse effect on the liver and kidney functions when administered at a low dosage (4 g/kg bw) to male and female rats in this study.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial and toxicological activities of five medicinal plant species from Cameroon Traditional Medicine

Nguedia

Kamga

Nsagha

et al. 2011

BMC Complement Altern Med

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BackgroundInfectious diseases caused by multiresistant microbial strains are on the increase. Fighting these diseases with natural products may be more efficacious. The aim of this study was to investigate the in vitro antimicrobial activity of methanolic, ethylacetate (EtOAc) and hexanic fractions of five Cameroonian medicinal plants (Piptadeniastum africana, Cissus aralioides, Hileria latifolia, Phyllanthus muellerianus and Gladiolus gregasius) against 10 pathogenic microorganisms of the urogenital and gastrointestinal tracts.MethodsThe fractions were screened for their chemical composition and in vivo acute toxicity was carried out on the most active extracts in order to assess their inhibitory selectivity.The agar well-diffusion and the micro dilution methods were used for the determination of the inhibition diameters (ID) and Minimum inhibitory concentrations (MIC) respectively on 8 bacterial species including two Gram positive species (Staphylococcus aureus, Enterococcus faecalis), and six Gram negative (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis, Shigella flexneri, Salmonella typhi) and two fungal isolates (Candida albicans, Candida krusei). The chemical composition was done according to Harbone (1976), the acute toxicity evaluation according to WHO protocol and the hepatic as well as serum parameters measured to assess liver and kidney functions.ResultsThe chemical components of each plant's extract varied according to the solvent used, and they were found to contain alkaloids, flavonoids, polyphenols, triterpens, sterols, tannins, coumarins, glycosides, cardiac glycosides and reducing sugars. The methanolic and ethylacetate extracts of Phyllanthus muellerianus and Piptadeniastum africana presented the highest antimicrobial activities against all tested microorganisms with ID varying from 8 to 26 mm and MIC from 2.5 to 0.31 mg/ml. The in vivo acute toxicity study carried out on the methanolic extracts of Phyllanthus muellerianus and Piptadeniastrum africana indicated that these two plants were not toxic. At the dose of 4 g/kg body weight, kidney and liver function tests indicated that these two medicinal plants induced no adverse effect on these organs.ConclusionThese results showed that, all these plant's extracts can be used as antimicrobial phytomedicines which can be therapeutically used against infections caused by multiresistant agents.Phyllanthus muellerianus, Piptadeniastum africana, antimicrobial, acute toxicity, kidney and liver function tests, Cameroon Traditional Medicine

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Section: Discussionmentioning

confidence: 99%

Antimicrobial and toxicological activities of five medicinal plant species from Cameroon Traditional Medicine

Nguedia

Kamga

Nsagha

et al. 2011

BMC Complement Altern Med

View full text Add to dashboard Cite

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“…In this context, an ethnobotanical survey was conducted by our research group among the pygmies Baka, a community living in the south of Cameroon and possessing a consolidated vernacular knowledge in the field of traditional medicine through the use of medicinal plants. Some of these medicinal plants were selected as candidates to validate their traditional use 19–23 . Among them, Diospyros bipindensis was identified as the most interesting plant for possible activity on PPARs receptors.…”

Section: Introductionmentioning

confidence: 99%

Betulinic acid is a PPARγ antagonist that improves glucose uptake, promotes osteogenesis and inhibits adipogenesis

Brusotti

Montanari

Capelli

et al. 2017

Sci Rep

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PPAR antagonists are ligands that bind their receptor with high affinity without transactivation activity. Recently, they have been demonstrated to maintain insulin-sensitizing and antidiabetic properties, and they serve as an alternative treatment for metabolic diseases. In this work, an affinity-based bioassay was found to be effective for selecting PPAR ligands from the dried extract of an African plant (Diospyros bipindensis). Among the ligands, we identified betulinic acid (BA), a compound already known for its anti-inflammatory, anti-tumour and antidiabetic properties, as a PPARγ and PPARα antagonist. Cell differentiation assays showed that BA inhibits adipogenesis and promotes osteogenesis; either down-regulates or does not affect the expression of a series of adipogenic markers; and up-regulates the expression of osteogenic markers. Moreover, BA increases basal glucose uptake in 3T3-L1 adipocytes. The crystal structure of the complex of BA with PPARγ sheds light, at the molecular level, on the mechanism by which BA antagonizes PPARγ, and indicates a unique binding mode of this antagonist type. The results of this study show that the natural compound BA could be an interesting and safe candidate for the treatment of type 2 diabetes and bone diseases.

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“…All fractions were tested for antimicrobial activity by minimum inhibitory concentration (MIC) against selected organisms Staphylococcus aureus , Psuedomonas aeruginosa and C. albicans to know which fraction shows more activity. [ 10 11 ] Chloramphenicol for bacteria and cotrimazole for fungi was used as positive control at concentrations ranging from 100 to 3.12 g/ml. Dimethyl sulfoxide (DMSO) was used as the solvent control.…”

Section: Methodsmentioning

confidence: 99%

Development of phytovesicles containing triterpenoids from Samadera indica

et al. 2015

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Introduction:Samadera indica belonging to Simaroubaceae family is being used traditionally for many diseases including arthritis, edema, itching, skin diseases, constipation, and general debility.Objective:The effectiveness of any drug delivery system depends upon its ability to deliver the active components at therapeutic level. In this study, a novel phyto vesicular formulation for the enhanced topical delivery of methanol extract of S. indica in order to treat skin infections was developed.Materials and Methods:The methanol extract fraction of leaves of S. indica which showed more antifungal activity was purified to separate an antifungal compound. Phytovesicles were formulated using the more antifungal fraction in order to treat topical and deep seated fungal infections. Pytovesicles were prepared using 1:2 molar ratio of antifungal triterpenoid from S. indica (AFTSI)-phosphatidylcholine by film hydration method.Results and Discussion:Chloroform 100% fraction of methanol extract of S. indica showed more activity against the fungus Candida albicans. Further purification gave a fraction with minimum inhibitory concentration value of 15.6 mg/ml against C. albicans and showed positive test for triterpenoids. The fraction was named as AFTSI. A compound (20 mg) was isolated from this fraction at an RF value. The phytovesicle gel formulated using AFTSI showed enhanced skin permeability and antifungal activity.Conclusion:The study demonstrated that the phytovesicular gel developed using methanol extract of S. indica would be beneficial for treating deep seated fungal infections.

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Antimicrobial properties of stem bark extracts from Phyllanthus muellerianus (Kuntze) Excell

Cited by 36 publications

References 10 publications

Antimicrobial and toxicological activities of five medicinal plant species from Cameroon Traditional Medicine

Antimicrobial and toxicological activities of five medicinal plant species from Cameroon Traditional Medicine

Betulinic acid is a PPARγ antagonist that improves glucose uptake, promotes osteogenesis and inhibits adipogenesis

Development of phytovesicles containing triterpenoids from Samadera indica

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