Antimicrobial properties of Cnestis ferruginea Vahl ex DC

Abstract

Scientific research into developing new antimicrobials from plants continues to be an interesting area for many scientists. This is because the resistance of microorganisms to anti-infective agents has affected a wide range of conditions, some of which are life-threatening. This study aimed to investigate the antimicrobial properties of Cnestis ferruginea (CF). Powdered roots of Cnestis ferruginea were extracted with petroleum ether (CFP), ethyl acetate (CFE) and methanol (CFM). The antimicrobial and microbial resistance modifying activity profiles of the extracts were studied against Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 4853, Staphylococcus aureus ATCC 25923, clinical strains of Methicillin-Resistant Staphylococcus aureus, Streptococcus pyogenes, Klebsiella pneumonia, Staphylococcus epidermidis, Proteus mirabilis and Candida albicans. CFP and CFE showed no activity against the test organisms. CFM had mean zones of growth inhibition in the range of 11.0 AE 0.5 to 22.17 AE 0.24 mm against the test organisms. The MIC of CFM was within the range of 0.31 and 5.0 mg/mL, with MBC/MFC range of 2.5-20.0 mg/mL. The time-kill kinetics studies showed CFM is a static agent. At sub-inhibitory concentrations, CFM was able to increase the susceptibility of the test organisms to standard antibiotics from the range of 1-8 folds. CFM reduced the formation of biofilms from 100% to 56.59%, 62.33%, 65.89% and 71.88% against K. pneumonia, S. aureus, E. coli and P. aeruginosa, respectively. The findings of this study show that C. ferruginea possesses antimicrobial activity and therefore gives credence to its folkloric use.

Evaluation of Dermal Toxicity and Wound Healing Activity of Cnestis ferruginea Vahl ex DC

“…Plant extracts with antimicrobial activity have also been reported to promote wound healing since infected or colonized wounds usually delay the healing process and sometimes lead to chronic wounds [ 8 ]. Hence, the reported antimicrobial activity of C. ferruginea [ 46 ] may contribute to its wound healing activity Also, subinhibitory concentrations of the methanolic extract of C. ferruginea , tested for their ability to inhibit biofilms in four microorganisms namely P. aeruginosa , E. coli , S. aureus, and K. pneumoniae, were able to significantly reduce the formation of biofilms, P < 0.0001 in a study by Ankomah et al [ 46 ]. This is an indication that C. ferruginea may be a very good choice in managing wounds as it will be able to prevent or decrease the formation of biofilms in microorganisms, which usually makes the management of wounds challenging.…”

Isolation and Identification of Flavanone Derivative Eriodictyol from the Methanol Extract of Afzelia africana Bark and Its Antimicrobial and Antioxidant Activities

“…Te microbial strains that were investigated include Bacillus subtilis (ATCC 10004), Candida albicans 1 (ATCC 90028), fuconazoleresistant Candida albicans 2 (clinical strain), Escherichia coli (NCTC), Klebsiella pneumonia (NCTC 13440), 12241), methicillin-resistant Staphylococcus aureus (NCTC 12493), Staphylococcus aureus (NCTC 12973), Pseudomonas aeruginosa (ATCC), Streptococcus mutans (ATCC 700610), 4853), and Salmonella typhi (ATCC 14028).2.7.3. Minimum Inhibitory Concentration (MIC).Te MIC of the compound was evaluated by employing the micro broth dilution method 96-well plate (Citotest Labware Manufacturing Co. Ltd, Jiangsu, China) according to the Clinical and Laboratory Standards Institute (CLSI) guidelines and[23][24][25][26] with minor modifcations. 10 mg/mL stock solution was prepared by weighing 10 mg and then dissolving in 1 mL of DMSO.…”