Antimicrobial Potential of Probiotic or Potentially Probiotic Lactic Acid Bacteria, the First Results of the International European Research Project PROPATH of the PROEUHEALTH Cluster

Vuyst, Luc De; Makras, Lefteris; Avonts, Lazlo; Holo, Helge; Qian, Yi; Servin, Alain L.; Fayol-Messaoudi, Domitille; Berger, Cédric N.; Ζουμποπούλου, Γεωργία; Tsakalidou, Effue; Sgouras, Dionyssios N.; Martinez-Gonzales, Beatriz; Panayotopoulou, Effie; Mentis, Αndreas; Smarandache, Diana; Savu, Lorand; Thonart, Philippe; Nes, Ingolf F.

doi:10.3402/mehd.v16i2-3.7936

Cited by 5 publications

(8 citation statements)

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“…Inter-strain differences in the anti-H. pylori activities of LB cultures were observed at both low and neutralized pH. Similarly, strain-dependant activity of Lactobacillus amylovorus, L. casei Shirota and Lactobacillus salivarius against H. pylori has been observed (Kim et al 2003;De Vuyst et al 2004;Ryan et al 2008 In the present study, the most active strains were LB2 and LB3. LB2 and LB3, taken together, failed to inhibit only 6AE7% (2 of 30) of the H. pylori strains tested at low pH and 20% (6 of 30) of the H. pylori strains at neutralized pH.…”

Section: Discussionsupporting

confidence: 73%

“…Similarly, strain‐dependant activity of Lactobacillus amylovorus, L. casei Shirota and Lactobacillus salivarius against H. pylori has been observed (Kim et al. 2003; De Vuyst et al. 2004; Ryan et al.…”

Section: Discussionmentioning

confidence: 88%

“…This activity could be associated with cell wall component, bacteriocin, bacteriocins-like substances or other bioactive substances. Several studies have reported that probiotic factors other than lactic acid, including bacteriocins, peroxides, proteinases, exopolysaccharides and cell wall components could exert antibacterial effects (Iliev et al 2001;Chatterjee et al 2003;Kim et al 2003;De Vuyst et al 2004;Rokka et al 2006). Thirty-six (12%) of 300 strains of the collection of ELBY Bulgaricum, including LB and L. helveticus strains, have been found to produce bacteriocins (Miteva et al 1998).…”

Section: Discussionmentioning

confidence: 99%

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Anti-Helicobacter pyloriactivity ofLactobacillus delbrueckiisubsp.bulgaricusstrains: preliminary report

Boyanova

Stephanova-Kondratenko

Mitov

2009

Letters in Applied Microbiology

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Aims: To evaluate the activities of six Lactobacillus delbrueckii subsp. bulgaricus (LB) strains against 30 Helicobacter pylori strains by agar-well diffusion method. Methods and Results: LB cultures [4 · 10 8 -4 · 10 9 CFU ml )1 ) either were prepared in milk at their native pH, 3AE8-5AE0, or were adjusted to pH 6AE4-7AE7. At low and neutralized pH, LB strains inhibited the growth by 40-86AE7% and 16AE7-66AE7% of H. pylori strains, respectively. LB activity was strain-dependent. At low and neutralized pH, one and five H. pylori strains, respectively, were not inhibited by any LB strain. LB2 and LB3, taken together, were active against most metronidazole and clarithromycin resistant strains. Conclusions: All LB strains inhibited a number of H. pylori strains, including also antibiotic resistant strains. LB activity was strain-dependent and better at low pH. At low pH values, the most active LB strains were LB1, LB2 and LB3, inhibiting 86AE7% of H. pylori strains, while at neutralized pH values, the most active LB strains were LB2 and LB3, inhibiting 53AE3 and 66AE7% of H. pylori strains, respectively. Significance and Impact of the Study: LB could be utilized in the treatment or prophylaxis of H. pylori infection and warrants clinical investigations.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

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Anti-Helicobacter pyloriactivity ofLactobacillus delbrueckiisubsp.bulgaricusstrains: preliminary report

Boyanova

Stephanova-Kondratenko

Mitov

2009

Letters in Applied Microbiology

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“…The properties of Lactobacillus strains of cheese origin, including the antibacterial activity, have been poorly documented. The strain L. plantarum ACA‐DC287 is a Xynotyri cheese isolate that has been selected from amongst 123 Lactobacillus strains isolated from a range of Greek food products, including raw milk, sour milk, yoghurt, Bulgarian‐type yoghurt, cheese, fresh Feta curd, Feta brine, Feta cheese, Kasseri cheese, Xynotyri cheese and Galotyri cheese, on the basis of their inhibitory activity against the gram‐negative pathogen Salmonella typhimurium using the well‐diffusion assay (De Vuyst et al. 2004).…”

Section: Introductionmentioning

confidence: 99%

The Lactobacillus plantarum strain ACA-DC287 isolated from a Greek cheese demonstrates antagonistic activity in vitro and in vivo against Salmonella enterica serovar Typhimurium

et al. 2007

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Aims: The purpose of this study was to investigate the antibacterial activity of the Xynotyri cheese isolate Lactobacillus plantarum ACA‐DC287 using a set of in vitro and in vivo assays. Methods and Results: The co‐culture of L. plantarum strain ACA‐DC287 and Salmonella enterica serovar Typhimurium strain SL1344 results in the killing of the pathogen. The killing activity was produced mainly by non‐lactic acid molecule(s) that were present in the cell‐free culture supernatant of the L. plantarum strain ACA‐DC287. The culture of the L. plantarum strain ACA‐DC287 inhibited the penetration of S. typhimurium SL1344 into cultured human enterocyte‐like Caco‐2/TC7 cells. In conventional mice infected with S. typhimurium SL1344, the intake of L. plantarum strain ACA‐DC287 results in a decrease in the levels of Salmonella associated with intestinal tissues or those present in the intestinal contents. In germ‐free mice, the L. plantarum strain ACA‐DC287 colonized the gastrointestinal tract. Conclusions: The L. plantarum strain ACA‐DC287 strain exerts anti‐Salmonella activity similar that of the established probiotic strains Lactobacillus rhamnosus GG, Lactobacillus casei Shirota YIT9029 and Lactobacillus johnsonii La1. Significance and Impact of the Study: The observation that a selected cheese Lactobacillus strain exerted antibacterial activity that was similar to those of probiotic Lactobacillus strains, is of interest for the use of this strain as an adjunct strain for the production of health‐giving cheeses.

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“…The probiotic bacterium Lactobacillus johnsonii NCC 533 possesses antimicrobial activities against several pathogens (Bernet et al , 1994; Fayol‐Messaoudi et al , 2005; Sgouras et al , 2005) and the parasite Giardia intestinalis (Humen et al , 2005). A recent investigation into the mechanism(s) by which NCC 533 kills Salmonella enterica serovar Typhimurium (Fayol‐Messaoudi et al , 2005) concluded that pH reduction, lactic acid and bacteriocin (Allison & Klaenhammer, 1996; De Vuyst et al , 2004) production were not sufficient and that another, as yet unidentified activity was responsible. Analysis of the predicted metabolic activities encoded within the genome sequence of NCC 533 (Pridmore et al , 2004) for alternative antimicrobial functions identified potential genes LJ1853 , LJ1826 and LJ1254 plus LJ1255 encoding for enzymes that could mediate the production of the known antimicrobial agent hydrogen peroxide (H 2 O 2 ), a compound not investigated by Fayol‐Messaoudi et al (2005).…”

Section: Introductionmentioning

confidence: 99%

Hydrogen peroxide production by Lactobacillus johnsonii NCC 533 and its role in anti-Salmonella activity

Pridmore

Pittet

Praplan

et al. 2008

FEMS Microbiology Letters

136

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The human intestinal isolate Lactobacillus johnsonii NCC 533 (La1) is a probiotic strain with well-documented antimicrobial properties. Previous research has identified the production of lactic acid and bacteriocins as important factors, but that other unidentified factors are also involved. We used the recently published genome sequence of L. johnsonii NCC 533 to search for novel antipathogen factors and identified three potential gene products that may catalyze the synthesis of the known antimicrobial factor hydrogen peroxide, H(2)O(2). In this work, we confirmed the ability of NCC 533 as well as eight different L. johnsonii strains and Lactobacillus gasseri to produce H(2)O(2) when resting cells were incubated in the presence of oxygen, and that culture supernatant containing NCC 533-produced H(2)O(2) was effective in killing the model pathogen Salmonella enterica serovar Typhimurium SL1344 in vitro.

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Antimicrobial Potential of Probiotic or Potentially Probiotic Lactic Acid Bacteria, the First Results of the International European Research Project PROPATH of the PROEUHEALTH Cluster

Cited by 5 publications

References 0 publications

Anti-Helicobacter pyloriactivity ofLactobacillus delbrueckiisubsp.bulgaricusstrains: preliminary report

Anti-Helicobacter pyloriactivity ofLactobacillus delbrueckiisubsp.bulgaricusstrains: preliminary report

The Lactobacillus plantarum strain ACA-DC287 isolated from a Greek cheese demonstrates antagonistic activity in vitro and in vivo against Salmonella enterica serovar Typhimurium

Hydrogen peroxide production by Lactobacillus johnsonii NCC 533 and its role in anti-Salmonella activity

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