Antimicrobial potential of a marine seaweedAsparagopsis taxiformis againstLeptospira javanica isolates of rodent reservoirs

Abstract

In this present investigation pharmacologically active compounds were isolated from red algae (Asparagopsis taxiformis) and their efficacy was evaluated against the Leptospira javanica isolates of rodent carriers. The GC-MS analysis of the purified compound revealed the presence of 4,5-dimethyl-1H-pyrrole-2-carboxylic acid ethyl ester (56.012%), fattyacids, 14-methyl-pentadecanoic acid methyl ester (26.6%), octadecanoic acid methyl ester (8.46%), octadec-9-enoic acid 2,3-dihydroxy-propyl ester (4.11%), 9-octadecanoic acid, methyl ester (4.535%) and trace amount of chlorobenzene (0.09%). MICs and MBCs of the purified compound against pathogenic leptospiral strains belonging to 14 serovars and 11 isolates belonging to serovar javanica of Leptospira borgpetersenii were determined in a range of 100-1600 μg/ml. The antibiotics penicillin and doxycycline were used as the standards for the efficiency determination of the seaweed extract against the leptospiral reference serovars and isolates. The minimal inhibitory concentration of penicillin and doxycyline were in the range of 25-200 μg/ml. The seaweed active fraction exhibited comparable MIC and MBC values with that of the standard antibiotic doxycycline. In the present study the seaweed compound has been developed to apply for the 2 nd nodal point of transmission cycle, the environment. Thus the present study draws the development of a novel drug to treat leptospires particularly in environments augmented with rodent carriers.

Radical scavenging activity of lipids from seaweeds isolated by solid-liquid extraction and supercritical fluids

“…Indeed, fatty acids inhibit enzyme activities, disrupt the electron transport chain and oxidative phosphorylation, and interfere with cellular energy production in microbes. They also damage nutrient uptake, generate peroxidation and auto-oxidation degradation products or direct lyse bacterial cells (Lee et al, 2009;Vedhagiri et al, 2009;Plouguerné et al, 2013). Many fatty acids demonstrate good antioxidant activities (Henry et al, 2002).…”

CARBON/NUTRIENT BALANCE IN RELATION TO BIOMASS PRODUCTION AND HALOGENATED COMPOUND CONTENT IN THE RED ALGA ASPARAGOPSIS TAXIFORMIS (BONNEMAISONIACEAE)1

“…This cocktail of metabolites is sequestered in specialized structures known as gland cells and may represent 2%-6% of the algal dry weight (Wolk 1968, Fenical 1975, Paul et al 2006). The high diversity and quantity of halogenated metabolites probably relate to the remarkable antimicrobial activity shown by Asparagopsis extracts when tested against human and animal pathogens (Bansemir et al 2006, Salvador et al 2007, Vedhagiri et al 2009). Since their discovery, research on the biological properties of halogenated compounds has shown antibacterial, antiviral, antifungal, anti-inflammatory, antifouling, cytotoxic, ichthyotoxic, and insecticidal activity (Blunt et al 2009).…”

Mycobiota associated with the rhodophyte alien species Asparagopsis taxiformis (Delile) Trevisan de Saint‐Léon in the Mediterranean Sea

“…), spirochaete bacteria of clinical importance (Vedhagiri et al . ), fish and shellfish pathogenic bacteria (Genovese et al . ), shrimp Vibrio pathogens and plant pathogenic fungi (Manilal et al .…”