Antimicrobial polycaprolactone/polyethylene glycol embedded lysozyme coatings of Ti implants for osteoblast functional properties in tissue engineering

Visan, Anita Ioana; Cristescu, R.; Stefan, N.; Miroiu, Marimona; Niță, Cristina; Socol, Marcela; Florica, Camelia; Rasoga, Oana; Zgura, Irina; Sima, Livia Elena; Chirițoiu, Marioara; Chifiriuc, Mariana Carmen; Holban, Alina Maria; Mihăilescu, I. N.; Socol, G.

doi:10.1016/j.apsusc.2016.11.129

Cited by 32 publications

(25 citation statements)

References 22 publications

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“…Preservation of the appropriate surface roughness and porosity of the biomaterial is, however, an important aspect during the colonization of implants by host cells (in example osteoblasts or fibroblasts) as well. Many studies prove that maintenance of the appropriate biomaterial roughness structure accelerates cell adhesion processes and increases biomechanical stability within the tissue covering the implant [63][64][65].…”

Section: Discussionmentioning

confidence: 99%

Serine Protease Inhibitors—New Molecules for Modification of Polymeric Biomaterials

et al. 2020

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Three serine protease inhibitors (AEBSF, soy inhibitor, α1-antitrypsin) were covalently immobilized on the surface of three polymer prostheses with the optimized method. The immobilization efficiency ranged from 11 to 51%, depending on the chosen inhibitor and biomaterial. The highest activity for all inhibitors was observed in the case of immobilization on the surface of the polyester Uni-Graft prosthesis, and the preparations obtained showed high stability in the environment with different pH and temperature values. Modification of the Uni-Graft prosthesis surface with the synthetic AEBSF inhibitor and human α1-antitrypsin inhibited the adhesion and multiplication of Staphylococcus aureus subs. aureus ATCC® 25923TM and Candida albicans from the collection of the Department of Genetics and Microbiology, UMCS. Optical profilometry analysis indicated that, after the immobilization process on the surface of AEBSF-modified Uni-Graft prostheses, there were more structures with a high number of protrusions, while the introduction of modifications with a protein inhibitor led to the smoothing of their surface.

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Section: Discussionmentioning

confidence: 99%

Serine Protease Inhibitors—New Molecules for Modification of Polymeric Biomaterials

et al. 2020

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“…This was followed by the subsequent calculation of the tangent to the drop at the liquid-solid vapor interface. The camera was positioned to observe the droplet under an angle of about 2 • -3 • with respect to the plane of sample surface supporting the droplet [14]. The measurements were conducted in a constant temperature (25 • C) and relative humidity (50%) conditions.…”

Section: Physico-chemical Characterization Of Pcl-blend-peg Coatingsmentioning

confidence: 99%

“…Owing to its high permeability for small drug molecules [10], the aliphatic polyester, PCL represents an important candidate for fabricating carriers for drug delivery applications, including: microparticles [11], nanoparticles [12], microspheres [13], coatings [14], and scaffolds [15]. However, due to its intrinsic high hydrophobicity and crystallinity, PCL degrades rather slowly.…”

Section: Introductionmentioning

confidence: 99%

Long-Term Evaluation of Dip-Coated PCL-Blend-PEG Coatings in Simulated Conditions

et al. 2020

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Our study focused on the long-term degradation under simulated conditions of coatings based on different compositions of polycaprolactone-polyethylene glycol blends (PCL-blend-PEG), fabricated for titanium implants by a dip-coating technique. The degradation behavior of polymeric coatings was evaluated by polymer mass loss measurements of the PCL-blend-PEG during immersion in SBF up to 16 weeks and correlated with those yielded from electrochemical experiments. The results are thoroughly supported by extensive compositional and surface analyses (FTIR, GIXRD, SEM, and wettability investigations). We found that the degradation behavior of PCL-blend-PEG coatings is governed by the properties of the main polymer constituents: the PEG solubilizes fast, immediately after the immersion, while the PCL degrades slowly over the whole period of time. Furthermore, the results evidence that the alteration of blend coatings is strongly enhanced by the increase in PEG content. The biological assessment unveiled the beneficial influence of PCL-blend-PEG coatings for the adhesion and spreading of both human-derived mesenchymal stem cells and endothelial cells.

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“…On the other hand, polycaprolactone is significantly biodegradable and biocompatible polyester with 60°C melting point. The most common use of polycaprolactone is in biomedical applications like implants, drug delivery systems, and tissue engineering …”

Section: Introductionmentioning

confidence: 99%

“…The most common use of polycaprolactone is in biomedical applications like implants, drug delivery systems, and tissue engineering. [12][13][14] Carbon nanotubes (CNTs) are carbon allotropes with different length and diameter cylindrical nanostructure with amazing mechanical, electrical, thermal, and adsorption properties. [15][16][17][18] Versatile outstanding applications of CNTs were used in many industrial sectors and on industrial scale such as lithium ion battery, 19 drug delivery system, 20 biomedical applications, 21 and manufactured implants.…”

Section: Introductionmentioning

confidence: 99%

π‐Plasmon absorbance films of carboxylic functionalized CNTs coupled with renewable PGP platforms

Fares

2018

Polymers for Advanced Techs

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π‐Plasmon absorbance films of carboxylic functionalized multiwall carbon nanotubes (CNTs) coupled with renewable and recycled polycaprolactone grafted pectin (PGP) platforms as successful alternative for ordinary nondegradable platforms were investigated. Characterization of the synthesized carboxylic functionalized CNTs was performed using 1H NMR and attenuated total reflectance Fourier transform infrared for structural identification, thermogravimetric analysis and derivative thermogravimetric analysis for thermal stability, and X‐ray powder diffraction for crystal structure, whereas the characterization of prepared PGP was done by means of attenuated total reflectance Fourier transform infrared for chemical structure, differential scanning calorimetry for melting endotherms of polycaprolactone and high crystalline structure of PGP, and thermogravimetric analysis and derivative thermogravimetric analysis for thermal stability of PGP. Fabrication of water‐dispersed carboxylic functionalized CNTs coupled with PGP films was performed by casting technique in the presence of Ca2+ as cross‐linker. The thin films were tested for π‐plasmon absorbance using UV‐Vis spectrometry. Different fractions of carboxylic functionalized CNTs and PGP films demonstrated π‐plasmon absorbance broad peaks at λmax = 232 nm, which corresponded to 5.36 eV. The fabrication of novel films from renewable recycled PGP platform and advanced carboxylic functionalized CNTs properties will be the key features for many of next forthcoming technologies. The PGP considered as environment‐friendly and easily degradable platforms will be a successful alternative for conventional nondegradable electronic platforms, and water‐dispersed carboxylic functionalized CNTs with advanced properties will be finding accelerating executive applications.

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Antimicrobial polycaprolactone/polyethylene glycol embedded lysozyme coatings of Ti implants for osteoblast functional properties in tissue engineering

Cited by 32 publications

References 22 publications

Serine Protease Inhibitors—New Molecules for Modification of Polymeric Biomaterials

Serine Protease Inhibitors—New Molecules for Modification of Polymeric Biomaterials

Long-Term Evaluation of Dip-Coated PCL-Blend-PEG Coatings in Simulated Conditions

π‐Plasmon absorbance films of carboxylic functionalized CNTs coupled with renewable PGP platforms

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