Antimicrobial photodynamic therapy with curcumin on methicillin-resistant Staphylococcus aureus biofilm

Ribeiro, Isabelle de Paula; Pinto, Juliana Guerra; Souza, Beatriz Müller Nunes; Miñán, Alejandro; Ferreira‐Strixino, Juliana

doi:10.1016/j.pdpdt.2022.102729

Cited by 25 publications

(10 citation statements)

References 49 publications

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“…This result may be also explained by low concentration of CUR loaded in the micelles and the micelles’ negative charge. Other studies verified reduction in the metabolic activity of MRSA and P. aerugonosa biofilms using higher concentrations of free CUR ( Abdulrahman et al, 2020 ; Ribeiro et al, 2022 ). For C. albicans biofilm, only CUR-loaded F127 micelles associated with blue light reduced the metabolic activity by 59%.…”

Section: Discussionmentioning

confidence: 75%

Photo-responsive polymeric micelles for the light-triggered release of curcumin targeting antimicrobial activity

et al. 2023

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Nanocarriers have been successfully used to solubilize, deliver, and increase the bioavailability of curcumin (CUR), but slow CUR release rates hinder its use as a topical photosensitizer in antimicrobial photodynamic therapy. A photo-responsive polymer (PRP) was designed for the light-triggered release of CUR with an effective light activation-dependent antimicrobial response. The characterization of the PRP was compared with non-responsive micelles comprising Pluronics™ P123 and F127. According to the findings, the PRP formed photo-responsive micelles in the nanometric scale (< 100 nm) with a lower critical micelle concentration (3.74 × 10−4 M−1, 5.8 × 10−4 M−1, and 7.2 × 10−6 M−1 for PRP, F127, P123, respectively, at 25°C) and higher entrapment efficiency of CUR (88.7, 77.2, and 72.3% for PRP, F127, and P123 micelles, respectively) than the pluronics evaluated. The PRP provided enhanced protection of CUR compared to P123 micelles, as demonstrated in fluorescence quenching studies. The light-triggered release of CUR from PRP occurred with UV light irradiation (at 355 nm and 25 mW cm−2) and a cumulative release of 88.34% of CUR within 1 h compared to 80% from pluronics after 36 h. In vitro studies showed that CUR-loaded PRP was non-toxic to mammal cell, showed inactivation of the pathogenic microorganisms Candida albicans, Pseudomonas aeruginosa, and methicillin-resistant Staphylococcus aureus, and decreased biofilm biomass when associated with blue light (455 nm, 33.84 J/cm2). The findings show that the CUR-loaded PRP micelle is a viable option for antimicrobial activity.

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Section: Discussionmentioning

confidence: 75%

Photo-responsive polymeric micelles for the light-triggered release of curcumin targeting antimicrobial activity

et al. 2023

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“…Furthermore, pathogenicity mechanisms, such as the presence of an efflux pump, and biofilm formation are presented by S. aureus (15). Considering Tig and AtpA, proteins with a statistically different expression in our study, they also are related to S. aureus biofilm formation.…”

Section: Discussionmentioning

confidence: 99%

“…One point to be considered is the charge feature of PS that influence the different susceptibility between Gram-positive and Gram-negative bacteria. In general, cationic PSs perform better antimicrobial efficiency compared with anionic or neutral compounds since the positive charge bind to the negatively charged surface of Gram-negative (14)(15)(16). With this in mind, xanthene dyes that have anionic forms have shown different actions in Gram-negative and Gram-positive, being this group more susceptible (17)(18)(19)(20).…”

Section: Introductionmentioning

confidence: 99%

Proteomic Investigation over the Antimicrobial Photodynamic Therapy Mediated by Rose Bengal Against Staphylococcus aureus

Silva

Meneguello

Formagio

et al. 2022

Photochem & Photobiology

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In order, understanding the antimicrobial action of photodynamic therapy and how this technique can contribute to its application in the control of pathogens. The objective of the study was to employ a proteomic approach to investigate the protein profile of Staphylococcus aureus after antimicrobial photodynamic therapy mediated by rose bengal (RB‐aPDT). S. aureus was treated with RB (10 nmoL L−1) and illuminated with green LED (0.17 J cm−2) for cell viability evaluation. Afterward, proteomic analysis was employed for protein identification and bioinformatic tools to classify the differentially expressed proteins. The reduction in S. aureus after photoinactivation was ~2.5 log CFU mL−1. A total of 12 proteins (four up‐regulated and eight down‐regulated) correspond exclusively to alteration by RB‐aPDT. Functionally, these proteins are distributed in protein binding, structural constituent of ribosome, proton transmembrane transporter activity and ATPase activity. The effects of photodamage include alterations of levels of several proteins resulting in an activated stress response, altered membrane potential and effects on energy metabolism. These 12 proteins required the presence of both light and RB suggesting a unique response to photodynamic effects. The information about this technique contributes valuable insights into bacterial mechanisms and the mode of action of photodynamic therapy.

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“…[1][2][3][4][5] At this point, they account for 40% to 60% of all healthcare-associated infections and are frequent pathogens in the infections of surgical wounds, the dermis and the bloodstream causing sepsis. 6 Staphylococcus epidermidis is the most prevalent skin bacterium, and is oen regarded as a contaminant when isolated from infected wounds. 7 The impact, however, is not limited to the skin as the peptides generated by S. epidermidis can contribute to the proliferation of pathogenic bacteria and, as a consequence, antibiotic resistance genes.…”

Section: Introductionmentioning

confidence: 99%

Photodynamic toluidine blue-gold nanoconjugates as a novel therapeutic for Staphylococcal biofilms

Okkeh,

De Vita,

Bruni

et al. 2023

RSC Adv.

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Antimicrobial photodynamic therapy with curcumin on methicillin-resistant Staphylococcus aureus biofilm

Cited by 25 publications

References 49 publications

Photo-responsive polymeric micelles for the light-triggered release of curcumin targeting antimicrobial activity

Photo-responsive polymeric micelles for the light-triggered release of curcumin targeting antimicrobial activity

Proteomic Investigation over the Antimicrobial Photodynamic Therapy Mediated by Rose Bengal Against Staphylococcus aureus

Photodynamic toluidine blue-gold nanoconjugates as a novel therapeutic for Staphylococcal biofilms

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