Antimicrobial photodynamic therapy in rat experimental candidiasis: evaluation of pathogenicity factors of Candida albicans

Martins, Joyce da Silva; Junqueira, Juliana Campos; Faria, Raquel Lourdes; Santiago, Naiara Fonseca; Rossoni, Rodnei Dennis; Colombo, Carlos Eduardo Dias; Jorge, Antônio Olavo Cardoso

doi:10.1016/j.tripleo.2010.08.012

Cited by 54 publications

(58 citation statements)

References 36 publications

(45 reference statements)

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“…The three main components of PDT are visible light, FS and oxygen 16 . Due to the fact that they absorb light with high efficiency, in some region of the visible spectrum, some of the FSs are able to induce or participate in photochemical reactions and produce reactive oxygen molecules 5,14,[17][18] .…”

Section: Discussionmentioning

confidence: 99%

“…The studies of Dai et al 1 , Kato et al 4 , Teichert et al 6 , Pereira et al 7 , Souza et al 8 , Queiroga et al 9 , Munin et al 10 , Silva Martins et al 17 , Giroldo et al 22 and Souza et al 23 , investigated the effects of photodynamic therapy on the viability of Candida albicans using methylene blue as a photosensitizer. The results of these studies proved the antifungal effect of this treatment modality.…”

Section: Discussionmentioning

confidence: 99%

“…In the absence of light, methylene blue showed no cytotoxic effect on Candida albicans cells, corroborating with the aforementioned studies. Both the photosensitizer and the light are relatively harmless by itself, but when combined, in the presence of oxygen, cause selective destruction on the affected area 17,22 . According to Pereira & Maisch 14 and Ryskova et al 15 photosensitizing agents show no or minimal inherent toxicity and the cytotoxic effect only occurs after its illumination with specific wavelength light.…”

Section: Discussionmentioning

confidence: 99%

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Photoinactivation of Candida albicans using methylene blue as photosensitizer

Sousa

Queiroga

Kocerginsky³

et al. 2015

RGO, Rev. Gaúch. Odontol.

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ObjectiveTo evaluate the effect of photodynamic therapy in the inactivation of Candida albicans planktonic cells, using methylene blue, 150 mg/mL, as a photosensitizer. Methods Aliquots of 100 (μg/mL of the fungal suspension in a 10 Results At the concentration of 150 μg/mL, and time of 5 min incubation, the reduction was significant (p˂0.05). In the absence of irradiation, methylene blue produced no reduction in CFU/mL. Conclusion Photodynamic Therapy presented antifungal effect against Candida albicans and can be used as an adjunct to conventional treatment.Indexing terms: Candida albicans. Methylene blue. Photochemotherapy. RESUMO ObjetivoAvaliar o efeito da terapia fotodinâmica na inativação de células planctônicas de Candida albicans, empregando azul de metileno a 150 µg/ mL como fotossensibilizador. MétodosAlíquotas de 100 µg/mL da suspensão do fungo na concentração de 10 6 células/mL foram semeadas em placas de microtitulação com 96 poços, onde foi depositado o mesmo volume de azul de metileno, permanecendo por um período de pré-irradiação de 5 min. Em seguida, aplicou-se luz laser de baixa potência (comprimento de onda: 660 nm, potência: 100 mW e dose: 426 J/cm 2 ) por 128s, utilizando um Laser semicondutor portátil. Experimentos controles foram realizados, sem iluminação e na ausência de azul de metileno, outro na presença de azul de metileno sem iluminação e com iluminação, substituindo o fotossensibilizador por solução salina. De cada condição experimental, diluições em série (10 -1 a 10 -3 ) foram obtidas e alíquotas de 25 μL foram plaqueadas em duplicata em Agar Sabouraud Dextrose. Após este período, o número de unidades formadoras de colônias por mililitro (CFU/mL) foi determinado e os dados foram submetidos à análise de variância e teste de Kruskal Wallis (p <0,05).Resultados Na concentração de 150 μg/mL e tempo de 5 min de incubação, a redução foi significativa (p<0,05). Na ausência de irradiação, o azul de metileno não produziu redução de CFU/mL. ConclusãoA Terapia Fotodinâmica apresentou efeito antifúngico contra Candida albicans, podendo ser utilizada como coadjuvante ao tratamento convencional.Termos de indexação: Candida albicans. Azul de metileno. Fotoquimioterapia. Therefore, this study aims to evaluate, in vitro, the photosensitizing effects in Candida albicans with laser radiation, using methylene blue as a photosensitizer. Photoinactivation of Candida albicans using methylene blue as photosensitizer

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Photoinactivation of Candida albicans using methylene blue as photosensitizer

Sousa

Queiroga

Kocerginsky³

et al. 2015

RGO, Rev. Gaúch. Odontol.

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“…including the ability to change cell morphology, a capacity to adhere to epithelial cells as well as the potential to secrete extracellular enzymes such as phospholipases and aspartyl proteinases 6,22,35 .…”

Section: Introductionmentioning

confidence: 99%

Oral colonization by yeasts in HIV-positive patients in Brazil

Junqueira

Vilela

Rossoni

et al. 2012

Rev. Inst. Med. trop. S. Paulo

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INTRODUCTION: In HIV-infected patients, colonization of the oral cavity by potential pathogenic yeast may lead to development of systemic fungemia. We evaluated the prevalence of yeast in the oral cavity of Brazilian HIV-positive patients and verified whether or not the species characterized were enzymatically active. Furthermore, the species identified were tested for their susceptibility to antifungal treatment. METHODS: Patient saliva and oropharyngeal candidiasis samples were collected from 60 seropositive HIV patients and identified by the API20C system. Enzymatic activity was evaluated by the production of proteinase and phospholipase. Susceptibility to antifungal treatments were determined using the broth microdilution method. RESULTS: the most commonly isolated species were C. albicans (51.56%) followed by non-albicans Candida species (43.73%), Trichosporon mucoides (3.12%) and Kodamaea ohmeri (1.56%). Oral colonization by association of different species was observed in 42% of the patients. Enzymatic activity was verified in most of species isolated, except for C. glabrata, C. lusitaniae and C. guilliermondii. Resistance to Fluconazole and Amphotericin B was observed in isolates of C. albicans, C. glabrata, C. parapsilosis, C. krusei, and K. ohmeri. CONCLUSION: HIV-positive patients are orally colonized by single or multiple species of yeast that are occasionally resistant to Fluconazole or Amphotericin B.

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“…In vivo investigations have also demonstrated the photoinactivation of C. albicans using animal models [22][23][24], as well as treatment of patients with OC [25,26]. Hematoporphyrin derivatives (HpD) are among the earliest type of PS used [10], and light-emitting diodes (LED) have been successfully used for PDT due to their lower cost and easier technology than lasers [25,27].…”

Section: Introductionmentioning

confidence: 99%

The influence of photodynamic therapy parameters on the inactivation ofCandidaspp:in vitroandin vivostudies

Alves¹,

Mima²,

Dovigo³

et al. 2014

Laser Phys.

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The influence of parameters of photodynamic therapy (PDT), such as pre-irradiation time (PIT), on the inactivation of Candida spp. was assessed in vitro and in vivo. Suspensions of Candida albicans, Candida tropicalis, Candida krusei and Candida glabrata were treated with Photogem R , incubated for 5, 10 or 15 min and illuminated with a blue LED light. Colonies were cultivated and log values of CFU ml −1 were analyzed by ANOVA and Kruskall-Wallis test. For in vivo evaluation, immunosuppressed mice were inoculated with C. albicans. PDT was performed on the dorsum of the tongue by topical administration of Photogem R and illumination after 5, 10 or 15 min. C. albicans was recovered from the tongue and the number of CFU ml −1 was analyzed by ANOVA and Tukey test. Animals were killed and the tongues were surgically removed for histological analysis. Susceptibility of Candida spp. suspensions to PDT was in decreasing order: C. albicans = C. tropicalis < C. krusei < C. glabrata. No significant difference was observed among the different PIT ( p > 0.05), both in vivo and in vitro. A significant reduction ( p < 0.05) of log(CFU ml −1 ) of C. albicans from tongues of mice was observed with no adverse effects in the tissue. PDT was effective to inactivate in vitro Candida spp. and for reduction of C. albicans in vivo, independently of the PIT used.

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Antimicrobial photodynamic therapy in rat experimental candidiasis: evaluation of pathogenicity factors of Candida albicans

Abstract: Photodynamic therapy reduced the microscopic lesions of experimental candidiasis in rats and inhibited the proteinase activity of C. albicans.

Cited by 54 publications

References 36 publications

Photoinactivation of Candida albicans using methylene blue as photosensitizer

Photoinactivation of Candida albicans using methylene blue as photosensitizer

Oral colonization by yeasts in HIV-positive patients in Brazil

The influence of photodynamic therapy parameters on the inactivation ofCandidaspp:in vitroandin vivostudies

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