Antimicrobial photodynamic therapy assessment of three indocyanine green-loaded metal-organic frameworks against Enterococcus faecalis

Golmohamadpour, Azadeh; Bahramian, Bahram; Khoobi, Mehdi; Pourhajibagher, Maryam; Barikani, Hamid Reza; Bahador, Abbas

doi:10.1016/j.pdpdt.2018.08.004

Cited by 49 publications

(47 citation statements)

References 60 publications

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“…One of the most frequently explored reference genes in bacterial gene expression studies is 16S rRNA . The 16S rRNA gene has been used in gene expression analysis of several aPDI-treated species in vitro, including P. gingivalis and S. aureus 22 , 31 ; ex vivo, including E. faecalis 32 ; and in biofilm cultures, including E. faecalis, P. aeruginosa and Streptococcus mutans 33 – 35 . Unfortunately, in the cited references, there are no data on the stability of the selected genes under the studied conditions.…”

Section: Discussionmentioning

confidence: 99%

Validation of stable reference genes in Staphylococcus aureus to study gene expression under photodynamic treatment: a case study of SEB virulence factor analysis

Ogonowska

Nakonieczna

2020

Sci Rep

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Staphylococcal enterotoxin B (SEB), encoded by the seb gene, is a virulence factor produced by Staphylococcus aureus that is involved mainly in food poisoning and is known to act as an aggravating factor in patients with atopic dermatitis. Research results in animal infection models support the concept that superantigens, including SEB contribute to sepsis and skin and soft tissue infections. In contrast to antibiotics, antimicrobial photodynamic inactivation (aPDI) is a promising method to combat both bacterial cells and virulence factors. The main aims of this research were to (1) select the most stable reference genes under sublethal aPDI treatments and (2) evaluate the impact of aPDI on seb. Two aPDI combinations were applied under sublethal conditions: rose bengal (RB) and green light (λmax = 515 nm) and new methylene blue (NMB) and red light (λmax = 632 nm). The stability of ten candidate reference genes (16S rRNA, fabD, ftsZ, gmk, gyrB, proC, pyk, rho, rpoB and tpiA) was evaluated upon aPDI using four software packages—BestKeeper, geNorm, NormFinder and RefFinder. Statistical analyses ranked ftsZ and gmk (RB + green light) and ftsZ, proC, and fabD (NMB + red light) as the most stable reference genes upon photodynamic treatment. Our studies showed downregulation of seb under both aPDI conditions, suggesting that aPDI could decrease the level of virulence factors.

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Section: Discussionmentioning

confidence: 99%

Validation of stable reference genes in Staphylococcus aureus to study gene expression under photodynamic treatment: a case study of SEB virulence factor analysis

Ogonowska

Nakonieczna

2020

Sci Rep

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“…Recently, Golmohamadpour et al assessed the aPDT efficacy of three indocyanine green (ICG)-loaded MOFs against E. faecalis 24-h biofilms in human mandibular first premolars as the first report of ICG-MOF for aPDT in endodontic infections [54] (Table 2) . Moreover, the gene expression of E. faecalis as one of the reasons for plaque formation on teeth was considerably decreased in the presence of ICG-Fe101-after PDT treatment (hypothesized to be due to iron activity by itself), since this conjugate was the highest spacious carrier.…”

Section: Metal-organic Frameworkmentioning

confidence: 99%

“…Moreover, the gene expression of E. faecalis as one of the reasons for plaque formation on teeth was considerably decreased in the presence of ICG-Fe101-after PDT treatment (hypothesized to be due to iron activity by itself), since this conjugate was the highest spacious carrier. Final outcomes revealed ICG-Fe101 as a promising PS due their structural characteristics (strong stability and great carrier) as well as the antimicrobial activity of iron metal by itself [54].…”

Section: Metal-organic Frameworkmentioning

confidence: 99%

An Insight into Advanced Approaches for Photosensitizer Optimization in Endodontics—A Critical Review

Diogo

Faustino

Neves

et al. 2019

JFB

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Apical periodontitis is a biofilm-mediated disease; therefore, an antimicrobial approach is essential to cure or prevent its development. In the quest for efficient strategies to achieve this objective, antimicrobial photodynamic therapy (aPDT) has emerged as an alternative to classical endodontic irrigation solutions and antibiotics. The aim of the present critical review is to summarize the available evidence on photosensitizers (PSs) which has been confirmed in numerous studies from diverse areas combined with several antimicrobial strategies, as well as emerging options in order to optimize their properties and effects that might be translational and useful in the near future in basic endodontic research. Published data notably support the need for continuing the search for an ideal endodontic photosensitizer, that is, one which acts as an excellent antimicrobial agent without causing toxicity to the human host cells or presenting the risk of tooth discoloration. The current literature on experimental studies mainly relies on assessment of mixed disinfection protocols, combining approaches which are already available with aPDT as an adjunct therapy. In this review, several approaches concerning aPDT efficiency are appraised, such as the use of bacteriophages, biopolymers, drug and light delivery systems, efflux pump inhibitors, negative pressure systems, and peptides. The authors also analyzed their combination with other approaches for aPDT improvement, such as sonodynamic therapy. All of the aforementioned techniques have already been tested, and we highlight the biological challenges of each formulation, predicting that the collected information may encourage the development of other effective photoactive materials, in addition to being useful in endodontic basic research. Moreover, special attention is dedicated to studies on detailed conditions, aPDT features with a focus on PS enhancer strategies, and the respective final antimicrobial outcomes. From all the mentioned approaches, the two which are most widely discussed and which show the most promising outcomes for endodontic purposes are drug delivery systems (with strong development in nanoparticles) and PS solubilizers.

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“…Photodiagn Photocyn . 2018;23:331–338 124. Copyright (2018), with permission from Elsevier for Figure 5E. Abbreviations: AgNP, silver nanoparticle; PS, photosensitizer; Car, carnosine; CSRBnp, rose bengal functionalized chitosan nanoparticles; EDC, N-ethyl-N’-(3-dimethyl aminopropyl) carbodiimide; GO, Graphene oxide; HAp, hydroxyapatite; ICG, indocyanine green; MB, Methylene blue; MOF, metal-organic frameworks; NGO, nano-Graphene oxide; NHS, N -Hydroxysuccinimide; NPs, nanoparticles; PLGA, Poly lactic-co-glycolic acid; TBO, Toluidine blue O; UCNPs, upconversion nanoparticles.…”

Section: Application Of Apdt In Oral Infectious Diseases and Treatingmentioning

confidence: 99%

<p>Novel nanomaterial-based antibacterial photodynamic therapies to combat oral bacterial biofilms and infectious diseases</p>

Qi¹,

Chi²,

Sun³

et al. 2019

IJN

117

100

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Oral diseases such as tooth caries, periodontal diseases, endodontic infections, etc., are prevalent worldwide. The heavy burden of oral infectious diseases and their consequences on the patients’ quality of life indicates a strong need for developing effective therapies. Advanced understandings of such oral diseases, e.g., inflammatory periodontal lesions, have raised the demand for antibacterial therapeutic strategies, because these diseases are caused by viruses and bacteria. The application of antimicrobial photodynamic therapy (aPDT) on oral infectious diseases has attracted tremendous interest in the past decade. However, aPDT had a minimal effect on the viability of organized biofilms due to the hydrophobic nature of the majority of the photosensitizers (PSs). Therefore, novel nanotechnologies were rapidly developed to target the delivery of hydrophobic PSs into microorganisms for the antimicrobial performance improvement of aPDT. This review focuses on the state-of-the-art of nanomaterials applications in aPDT against oral infectious diseases. The first part of this article focuses on the cutting-edge research on the synthesis, toxicity, and therapeutic effects of various forms of nanomaterials serving as PS carriers for aPDT applications. The second part discusses nanomaterials applications for aPDT in treatments of oral diseases. These novel bioactive nanomaterials have demonstrated great potential to serve as carriers for PSs to substantially enhance the PDT therapeutic effects. Furthermore, the novel aPDT applications not only have exciting therapeutic potential to inhibit bacterial plaque-initiated oral diseases, but also have a wide applicability to other biomedical and tissue engineering applications.

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Antimicrobial photodynamic therapy assessment of three indocyanine green-loaded metal-organic frameworks against Enterococcus faecalis

Cited by 49 publications

References 60 publications

Validation of stable reference genes in Staphylococcus aureus to study gene expression under photodynamic treatment: a case study of SEB virulence factor analysis

Validation of stable reference genes in Staphylococcus aureus to study gene expression under photodynamic treatment: a case study of SEB virulence factor analysis

An Insight into Advanced Approaches for Photosensitizer Optimization in Endodontics—A Critical Review

<p>Novel nanomaterial-based antibacterial photodynamic therapies to combat oral bacterial biofilms and infectious diseases</p>

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