Antimicrobial photodynamic therapy (aPDT) with curcumin controls intradermal infection by Staphylococcus aureus in mice with type 1 diabetes mellitus: a pilot study

Muniz, Igor Pereira Ribeiro; Galantini, Maria Poliana Leite; Ribeiro, Israel Souza; Gonçalves, Caroline Vieira; Santos, Denisar Palmito dos; Moura, Tatyana Chagas; Silva, Emely Soares; Silva, Nathalia Rosa; Cipriano, Barbara Porto; Correia, Thiago Macêdo Lopes; Soares, Telma de Jesus; Freitas, Leandro Martins de; Costa, Dirceu Joaquim; Silva, Robson Amaro Augusto da

doi:10.1016/j.jphotobiol.2021.112325

Cited by 12 publications

(4 citation statements)

References 42 publications

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“…The extracts from Juniperus branches, leaves, and berries showed broad-spectrum antibacterial properties, especially for S. aureus (Taviano et al, 2013;Dziedzinski et al, 2020). In addition, as key phenols and podophyllotoxins of the extracts, catechin, curcumin, and podophyllotoxin were previously shown to have inhibitory activity against S. aureus and E. coli in previous studies (Rocha et al, 2018;Muniz et al, 2021). However, the content of the above compounds in the roots of the best inhibiting tissues was low.…”

Section: Anti-drug Resistant Bacteria Activity In Vitromentioning

confidence: 99%

Effects of age and tissue of Juniperus sabina L. on its phytochemical characteristics, anti-cholinesterase, antidiabetes, and anti-drug resistant bacteria activities

Xu,

Chen,

Luo

et al. 2023

Front. Plant Sci.

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Juniperus sabina L. is used in the traditional Chinese medicine (TCM) system to prevent or treat various diseases. However, only the leaves and branches are used as medicinal parts. The aim of this study was to compare the chemical characteristics of different tissues (leaves, branches, stems, and roots) of J. sabina at different ages by HPLC-MS and to evaluate the biological activity (enzyme inhibition, anti-drug-resistant bacteria). Total phenol (TPC) and total lignan (TLC) contents in J. sabina were determined by Folin-Ciocalteu method and UV spectrophotometry, respectively. High levels of total phenols (87.16 mg GAE/g dry weight) and total lignans (491.24 mg PPT/g dry weight) were detected in fifteen annual J. sabina roots and current year leaves, respectively. Eleven compounds, of which six were phenolic compounds and five were lignans, were identified and quantified by HPLC/HPLC-MS. Statistical analysis showed that the distribution and content of the detected compounds showed considerable variation among ages and tissues, and that the current year leaves of fifteen annual J. sabina could be used as a potential application site for the source of podophyllotoxin. Acetylcholinesterase (AChE) inhibitory activity was found to be the highest on the extracts of fifteen annual J. sabina current year leaves (47.37 μg/mL), while the highest inhibition towards butyrylcholinesterase (BChE) was observed for the extracts of seven annual J. sabina previous year leaves (136.3 μg/mL). And the second annual J. sabina current year stem’s extracts showed the best antidiabetic activity (anti-α-glucosidase, 62.59 μg/mL). In addition, the extracts of fifteen annual J. sabina roots (47.37 μg/mL) showed the highest anti-MRSA activity (31.25 μg/mL). Redundancy analysis (RDA) was conducted to clarify the factors affecting the biological activity of J. sabina, and its results showed that epicatechin and matairesinol showed positive promotion. This study provides a new perspective for understanding the chemical differences and comprehensive utilization of different tissues of J. sabina.

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Section: Anti-drug Resistant Bacteria Activity In Vitromentioning

confidence: 99%

Effects of age and tissue of Juniperus sabina L. on its phytochemical characteristics, anti-cholinesterase, antidiabetes, and anti-drug resistant bacteria activities

Xu,

Chen,

Luo

et al. 2023

Front. Plant Sci.

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“…However, the use of curcumin in aPDT is currently limited to local applications on superficial wounds, such as the skin and oral cavity, primarily due to its absorption of blue light within the light spectrum (300-500 nm), which has restricted tissue penetration capabilities. Muniz et al demonstrated that curcumin (100 µg), as a PS being activated ex vivo by LED (450 nm, 13.5 J/cm 2 ), effectively controlled Staphylococcus aureus (S. aureus) infections in mice with type 1 diabetes mellitus (36). Meńdez et al found that curcuminmediated aPDT effectively reduced the viability of microbial cells and compromised the vitality of intact biofilms of infected dentin caries microcosms (37).…”

Section: Curcuminmentioning

confidence: 99%

“…Paolillo FR et al discovered that a combination of curcumin (0.06 mL of 1.5% curcumin gel) and blue light (450 nm, 80 mW/cm 2 , at the dose of 60 J/cm 2 )-mediated aPDT, with artificial skin, accelerated bacterial inactivation (S. aureus 4.14 log10) and enhanced wound healing in Wistar rats without inducing adverse effects on the tissue (159). Muniz IPR et al demonstrated that ex vivo activation of curcumin (100 mg) by blue LED light (450 nm) at a fluence of 13.5 J/cm 2 effectively controlled S. aureus cutaneous infection in type I diabetic mice (36,160). Alam et al achieved significant eradication of Ampicillin-Resistant P. aeruginosa in the Caenorhabditis elegans (C. elegans) model by using HYP in conjunction with ampicillin and subsequent orange light treatment (48).…”

Section: In Vivo Apdt With Polyphenolsmentioning

confidence: 99%

Polyphenolic natural products as photosensitizers for antimicrobial photodynamic therapy: recent advances and future prospects

Wang,

Fekrazad

et al. 2023

Front. Immunol.

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Antimicrobial photodynamic therapy (aPDT) has become a potent contender in the fight against microbial infections, especially in the context of the rising antibiotic resistance crisis. Recently, there has been significant interest in polyphenolic natural products as potential photosensitizers (PSs) in aPDT, given their unique chemical structures and inherent antimicrobial properties. Polyphenolic natural products, abundant and readily obtainable from natural sources, are generally regarded as safe and highly compatible with the human body. This comprehensive review focuses on the latest developments and future implications of using natural polyphenols as PSs in aPDT. Paramount polyphenolic compounds, including curcumin, hypericin, quercetin, hypocrellin, celastrol, riboflavin, resveratrol, gallic acid, and aloe emodin, are elaborated upon with respect to their structural characteristics, absorption properties, and antimicrobial effects. Furthermore, the aPDT mechanism, specifically its targeted action on microbial cells and biofilms, is also discussed. Polyphenolic natural products demonstrate immense potential as PSs in aPDT, representing a promising alternate approach to counteract antibiotic-resistant bacteria and biofilm-related infections.

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“…In S. aureus infections, the host initiates an innate immune response primarily mediated by neutrophils (Rigby & DeLeo 2012, dos Santos et al 2018a, which is responsible for destroying and phagocytizing the bacteria, and migrating to the draining lymph node for antigen presentation (Hermida et al 2014, Muniz et al 2021. It is also known that the fluctuation of the number of circulating neutrophils in response to exercise depends on training intensity.…”

Section: Lipid Content Positively Correlated With the Bacterial Load ...mentioning

confidence: 99%

Physical activity reduces intradermal bacterial load in a murine model submitted to forced swim training – a pilot study

Galantini

Leal

Rodrigues

et al. 2022

An. Acad. Bras. Ciênc.

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Regular exercise is benefi cial to health. This study evaluated the effects of moderate and intense physical exercise modalities on intradermal infection by Staphylococcus aureus in a murine model. Mice that practiced moderate exercise had lower bacterial load on lymph nodes and less infl ammatory infi ltrate in dermis. They presented greater weight, however, less amount of epididymal fat: the weight was increased while they had fat diminished. A positive correlation was observed between lipid content and bacterial load in mice trained at moderate intensity. Animals that were under high intensity exercises presented superior bacterial load on the lymph nodes, increased neutrophil count and circulating lymphocytes, and had leukocyte recruitment to the dermis augmented, when compared to the ones in moderate exercise. These fi ndings suggest that moderate physical activity modulates the immune response in dermal infection caused by S. aureus in a murine model.

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Antimicrobial photodynamic therapy (aPDT) with curcumin controls intradermal infection by Staphylococcus aureus in mice with type 1 diabetes mellitus: a pilot study

Cited by 12 publications

References 42 publications

Effects of age and tissue of Juniperus sabina L. on its phytochemical characteristics, anti-cholinesterase, antidiabetes, and anti-drug resistant bacteria activities

Effects of age and tissue of Juniperus sabina L. on its phytochemical characteristics, anti-cholinesterase, antidiabetes, and anti-drug resistant bacteria activities

Polyphenolic natural products as photosensitizers for antimicrobial photodynamic therapy: recent advances and future prospects

Physical activity reduces intradermal bacterial load in a murine model submitted to forced swim training – a pilot study

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