Antimicrobial photodynamic inactivation of fungal biofilm using amino functionalized mesoporus silica-rose bengal nanoconjugate against Candida albicans

Parasuraman, Paramanantham; Antony, Asha P; Lal, Sham; Sharan, Alok; Syed, Asad; Ahmed, Mukhtar; Alarfaj, Abdullah A.; Siddhardha, Busi; Maaza, M.; Kaviyarasu, K.

doi:10.1016/j.sciaf.2018.e00007

Cited by 30 publications

(32 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of glycoconjugates observation confirmed that LED illumination could significantly reduce the concentration of glycoconjugates (Figure 3), especially at 25 • C ( Figure 3A). Paramanantham et al (2018) reported a 54.93% reduction in the exopolysaccharide content of biofilm after treatment with an amino-functionalized mesoporus silica-rose Bengal nanoconjugate using a Congo red binding assay. The mechanism of action of LED illumination against several intracellular substances has been reported and is thought to involve ROS generated by cells in response to LED light that may react with DNA, proteins, lipids, and other components to produce cytotoxic effects (Luksiene, 2010).…”

Section: Discussionmentioning

confidence: 99%

Inactivation Efficacy of 405 nm LED Against Cronobacter sakazakii Biofilm

et al. 2020

View full text Add to dashboard Cite

The objectives of this study were to evaluate the inactivation efficacy of a 405-nm light-emitting diode (LED) against Cronobacter sakazakii biofilm formed on stainless steel and to determine the sensitivity change of illuminated biofilm to food industrial disinfectants. The results showed that LED illumination significantly reduced the population of viable biofilm cells, showing reduction of 2.0 log (25°C), 2.5 log (10°C), and 2.0 log (4°C) between the non-illuminated and LED-illuminated groups at 4 h. Images of confocal laser scanning microscopy and scanning electron microscopy revealed the architectural damage to the biofilm caused by LED illumination, which involved destruction of the stereoscopic conformation of the biofilm. Moreover, the loss of biofilm components (mainly polysaccharide and protein) was revealed by attenuated total reflection Fourier-transformed infrared spectroscopy, and the downregulation of genes involved in C. sakazakii biofilm formation was confirmed by real time quantitative PCR analysis, with greatest difference observed in fliD. In addition, the sensitivity of illuminated-biofilm cells to disinfectant treatment was found to significantly increased, showing the greatest sensitivity change with 1.5 log reduction between non-LED and LED treatment biofilms in the CHX-treated group. These results indicated that 405 nm LED illumination was effective at inactivating C. sakazakii biofilm adhering to stainless steel. Therefore, the present study suggests the potential of 405 nm LED technology in controlling C. sakazakii biofilms in food processing and storage, minimizing the risk of contamination.

show abstract

Section: Discussionmentioning

confidence: 99%

Inactivation Efficacy of 405 nm LED Against Cronobacter sakazakii Biofilm

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In addition, prior experiments have shown that encapsulation and delivery of antifungal agents (e.g., herbal extracts and chemicals) using MSNs can significantly enhance their antifungal effects [ 111 , 112 ]. On this matter, MSNs, with an average pore size and diameter of 2 and 500 nm, respectively, were synthesized and modified with a photosensitizer (i.e., Rose Bengal (RB)) to be used in photodynamic therapy (PDT) for treating C. Albicans biofilms [ 113 ]. By emitting light, MSNs-RB exhibited high antimicrobial action against C. Albicans planktonic cells (reduction up to 88.7%) and biofilms (reduction up to 79.7%) as compared to RB-free MSNs.…”

Section: Multiple Roles Of Msns In Skin Wound Healingmentioning

confidence: 99%

Mesoporous Silica Nanoparticles and Mesoporous Bioactive Glasses for Wound Management: From Skin Regeneration to Cancer Therapy

et al. 2021

View full text Add to dashboard Cite

Exploring new therapies for managing skin wounds is under progress and, in this regard, mesoporous silica nanoparticles (MSNs) and mesoporous bioactive glasses (MBGs) offer great opportunities in treating acute, chronic, and malignant wounds. In general, therapeutic effectiveness of both MSNs and MBGs in different formulations (fine powder, fibers, composites etc.) has been proved over all the four stages of normal wound healing including hemostasis, inflammation, proliferation, and remodeling. The main merits of these porous substances can be summarized as their excellent biocompatibility and the ability of loading and delivering a wide range of both hydrophobic and hydrophilic bioactive molecules and chemicals. In addition, doping with inorganic elements (e.g., Cu, Ga, and Ta) into MSNs and MBGs structure is a feasible and practical approach to prepare customized materials for improved skin regeneration. Nowadays, MSNs and MBGs could be utilized in the concept of targeted therapy of skin malignancies (e.g., melanoma) by grafting of specific ligands. Since potential effects of various parameters including the chemical composition, particle size/morphology, textural properties, and surface chemistry should be comprehensively determined via cellular in vitro and in vivo assays, it seems still too early to draw a conclusion on ultimate efficacy of MSNs and MBGs in skin regeneration. In this regard, there are some concerns over the final fate of MSNs and MBGs in the wound site plus optimal dosages for achieving the best outcomes that deserve careful investigation in the future.

show abstract

“…The development of new formulations against Candida albicans is essential to overcome drawbacks as multiple resistance mechanisms developed by biofilms formation, which makes this pathogen resistant to drugs such as fluconazole and amphotericin B, and, consequently, making difficult to treat effectively these infections, contributing to high morbidity and mortality rates in immunocompromised patients (141,155). In light of this, Paramanantham et al .…”

Section: Fungal Infectionsmentioning

confidence: 99%

“…In light of this, Paramanantham et al . ( 141 ) reported a nanosystem based on MSNs modified with Rose Bengal (RB) as photodynamic therapy (PDT) as antimicrobial alternative to treat C. albicans biofilms. MSNs with average pores size and diameter of 2 and 500 nm, respectively, were amino functionalized aiming to increase the conjugation capacity of RB.…”

Section: Msns For Infectious Diseases Treatmentmentioning

confidence: 99%

Highlights in Mesoporous Silica Nanoparticles as a Multifunctional Controlled Drug Delivery Nanoplatform for Infectious Diseases Treatment

et al. 2020

View full text Add to dashboard Cite

Infectious diseases are a major global concern being responsible for high morbidity and mortality mainly due to the development and enhancement of multidrug-resistant microorganisms exposing the fragility of medicines and vaccines commonly used to these treatments. Taking into account the scarcity of effective formulation to treat infectious diseases, nanotechnology offers a vast possibility of groundbreaking platforms to design new treatment through smart nanostructures for drug delivery purposes. Among the available nanosystems, mesoporous silica nanoparticles (MSNs) stand out due their multifunctionality, biocompatibility and tunable properties make them emerging and actual nanocarriers for specific and controlled drug release. Considering the high demand for diseases prevention and treatment, this review exploits the MSNs fabrication and their behavior in biological media besides highlighting the most of strategies to explore the wide MSNs functionality as engineered, smart and effective controlled drug release nanovehicles for infectious diseases treatment.

show abstract

Antimicrobial photodynamic inactivation of fungal biofilm using amino functionalized mesoporus silica-rose bengal nanoconjugate against Candida albicans

Cited by 30 publications

References 42 publications

Inactivation Efficacy of 405 nm LED Against Cronobacter sakazakii Biofilm

Inactivation Efficacy of 405 nm LED Against Cronobacter sakazakii Biofilm

Mesoporous Silica Nanoparticles and Mesoporous Bioactive Glasses for Wound Management: From Skin Regeneration to Cancer Therapy

Highlights in Mesoporous Silica Nanoparticles as a Multifunctional Controlled Drug Delivery Nanoplatform for Infectious Diseases Treatment

Contact Info

Product

Resources

About