Antimicrobial peptides: Promising alternatives over conventional capture ligands for biosensor-based detection of pathogenic bacteria

Islam, Muhammad Amirul; Karim, Abdallah; Ethiraj, Baranitharan; Raihan, Topu; Kadier, Abudukeremu

doi:10.1016/j.biotechadv.2021.107901

Cited by 24 publications

(17 citation statements)

References 160 publications

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“…The most critical component for a sensor is the recognition element (referred to as receptor), which defines its selectivity and sensitivity. Among various recognition elements (e.g., antibody, , aptamer, antimicrobial peptides, , antibiotic, molecularly imprinted polymer , and boronate-affinity compound), antibodies are the dominant receptors owing to their distinguished affinity and specificity. However, natural antibodies are still limited by their high cost, poor stability, and ethical issues in animal experiments. , Thus, developing synthetic receptors as antibody substitutes holds significant importance.…”

mentioning

confidence: 99%

Dual Synthetic Receptor-Based Sandwich Electrochemical Sensor for Highly Selective and Ultrasensitive Detection of Pathogenic Bacteria at the Single-Cell Level

et al. 2023

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Sensitive and rapid detection of pathogenic bacteria is essential for effective source control and prevention of microbial infectious diseases. However, it remains a substantial challenge to rapidly detect bacteria at the single-cell level. Herein, we present an electrochemical sandwich sensor for highly selective and ultrasensitive detection of a single bacterial cell based on dual recognition by the bacteria-imprinted polymer film (BIF) and aptamer. The BIF was used as the capture probe, which was in situ fabricated on the electrode surface within 15 min via electropolymerization. The aptamer and electroactive 6-(Ferrocenyl)hexanethiol cofunctionalized gold nanoparticles (Au@Fc-Apt) were employed as the signal probe. Once the target bacteria were anchored on the BIF-modified electrode, the Au@Fc-Apt was further specifically bound to the bacteria, generating enhanced current signals for ultrasensitive detection of Staphylococcus aureus down to a single cell in phosphate buffer solution. Even in the complex milk samples, the sensor could detect as low as 10 CFU mL–1 of S. aureus without any complicated pretreatment except for 10-fold dilution. Moreover, the current response to the target bacteria was hardly affected by the coexisting multiple interfering bacteria, whose number is 30 times higher than the target, demonstrating the excellent selectivity of the sensor. Compared with most reported sandwich-type electrochemical sensors, this assay is more sensitive and more rapid, requiring less time (1.5 h) for the sensing interface construction. By virtue of its sensitivity, rapidity, selectivity, and cost-effectiveness, the sensor can serve as a universal detection platform for monitoring pathogenic bacteria in fields of food/public safety.

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confidence: 99%

Dual Synthetic Receptor-Based Sandwich Electrochemical Sensor for Highly Selective and Ultrasensitive Detection of Pathogenic Bacteria at the Single-Cell Level

et al. 2023

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“…In particular, the emergence of new ARGs has greatly weakened our traditional treatment options in the clinical setting, almost producing the dilemma of no available antibiotics. In the era of drug resistance, AMPs have attracted global attention as a new candidate drug for the treatment of infectious diseases due to the limited choice of existing antibiotic regimens [ 42 ]. However, high synthesis costs and poor anti-proteolytic ability are urgent challenges that prevent the clinical application of AMPs.…”

Section: Discussionmentioning

confidence: 99%

Truncated Pleurocidin Derivative with High Pepsin Hydrolysis Resistance to Combat Multidrug-Resistant Pathogens

et al. 2022

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The global prevalence of antimicrobial resistance calls for the development of novel antimicrobial agents, particularly for these orally available drugs. Structural modifications of the natural antimicrobial peptides (AMPs) provide a straightforward approach to develop potent antimicrobial agents with high specificity and low toxicity. In this study, we truncated 11-amino-acids at the C-terminus of Pleurocidin, an AMP produced by Pleuronectes americanus, and obtained four peptide analogues termed GK-1, GK-2, GK-3 and GK-4. Minimum inhibitory concentration (MIC) tests showed that GK-1 obtained by direct truncation of Pleurocidin has no antibacterial activity, while GK-2, GK-3 and GK-4 show considerable antibacterial activity with Pleurocidin. Notably, GK-4 displays rapid bacteriostatic activity, great stability and low hemolysis, as well as enhanced hydrolytic resistance to pepsin treatment. Mechanistic studies showed that GK-4 induces membrane damage by interacting with bacterial membrane-specific components, dissipates bacterial membrane potential and promotes the generation of ROS. SEM and CD analysis further confirmed the ability of GK-4 to resist pepsin hydrolysis, which may be attributed to its stable helicity structure. Collectively, our findings reveal that GK-4 is a potential orally available candidate to treat infections caused by multidrug-resistant pathogens.

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“…The second step then leads to bacterial death by destroying the membrane integrity of targets and/or inserting the microbial membrane into the cell (Figure 2E). 76 This target-independent antibacterial mechanism of AMPs can overcome drug resistance while having broad-spectrum antimicrobial activity. Therefore, based on the high affinity and high stability of AMPs to bacteria, their properties as recognition molecules have received extensive attention.…”

Section: Biochemical Modification Of Mnpsmentioning

confidence: 99%

“…AMPs have an amphiphilic structure, with the hydrophilic fragment interacting with the negatively charged phospholipid headgroup and hydrophobic peptide domain interacting with lipid bilayer. The second step then leads to bacterial death by destroying the membrane integrity of targets and/or inserting the microbial membrane into the cell (Figure E) . This target-independent antibacterial mechanism of AMPs can overcome drug resistance while having broad-spectrum antimicrobial activity.…”

Section: Biochemical Modification Of Mnps To Isolate Bacteria From Foodmentioning

confidence: 99%

Magnetic Nanoseparation Technology for Efficient Control of Microorganisms and Toxins in Foods: A Review

Kang

Shi

Sun

et al. 2022

J. Agric. Food Chem.

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Outbreaks of foodborne diseases mediated by food microorganisms and toxins remain one of the leading causes of disease and death worldwide. It not only poses a serious threat to human health and safety but also imposes a huge burden on health care and socioeconomics. Traditional methods for the removal and detection of pathogenic bacteria and toxins in various samples such as food and drinking water have certain limitations, requiring a rapid and sensitive strategy for the enrichment and separation of target analytes. Magnetic nanoparticles (MNPs) exhibit excellent performance in this field due to their fascinating properties. The strategy of combining biorecognition elements with MNPs can be used for fast and efficient enrichment and isolation of pathogens. In this review, we describe new trends and practical applications of magnetic nanoseparation technology in the detection of foodborne microorganisms and toxins. We mainly summarize the biochemical modification and functionalization methods of commonly used magnetic nanomaterial carriers and discuss the application of magnetic separation combined with other instrumental analysis techniques. Combined with various detection techniques, it will increase the efficiency of detection and identification of microorganisms and toxins in rapid assays.

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Antimicrobial peptides: Promising alternatives over conventional capture ligands for biosensor-based detection of pathogenic bacteria

Cited by 24 publications

References 160 publications

Dual Synthetic Receptor-Based Sandwich Electrochemical Sensor for Highly Selective and Ultrasensitive Detection of Pathogenic Bacteria at the Single-Cell Level

Dual Synthetic Receptor-Based Sandwich Electrochemical Sensor for Highly Selective and Ultrasensitive Detection of Pathogenic Bacteria at the Single-Cell Level

Truncated Pleurocidin Derivative with High Pepsin Hydrolysis Resistance to Combat Multidrug-Resistant Pathogens

Magnetic Nanoseparation Technology for Efficient Control of Microorganisms and Toxins in Foods: A Review

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