Antimicrobial Peptides from Black Soldier Fly (Hermetia illucens) as Potential Antimicrobial Factors Representing an Alternative to Antibiotics in Livestock Farming

Xia, Jing; Ge, Chaorong; Yao, Huaiying

doi:10.3390/ani11071937

Cited by 77 publications

(55 citation statements)

References 124 publications

(151 reference statements)

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“…Wang and Shelomi [8] have also highlighted this advantage with specific reference to BSFL. Apart from that, BSFL has also been bio-prospected for its potential as a source of antimicrobial peptides [9][10][11][12], chitin [13][14][15] and lipids [16].…”

Section: Introductionmentioning

confidence: 99%

Nutritional value of black soldier fly (Hermetia illucens) larvae processed by different methods

et al. 2022

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Nutritional value of black soldier fly (Hermetia illucens) larvae (BSFL) processed by three different methods of treatment was compared. The resulting products were the spray-dried BSFL (SPR), oven-dried BSFL 1 (OVN1) and oven-dried BSFL 2 (OVN2). Proximate chemical composition, and profiles of amino acids, fatty acids, minerals, heavy metals, vitamins and nucleotides were analysed and compared. The tested BSFL meals were considered to have a good profile of essential amino acids (EAAs), with leucine, lysine, valine, and histidine being the dominant EAAs. Their content of saturated fatty acids exceeded that of the unsaturated fatty acids. Vitamins B1, B2, and C were also present in the samples. Minerals such as calcium, potassium, phosphorus, sodium, magnesium, zinc, iron, manganese and copper were found to be in adequate amounts in almost all the samples. Heavy metals in the BSFL meals were mostly below 1g kg-1. Nucleotides such as inosine monophosphate and uridine monophosphate occurred in all the BSFL meals. Other nucleotides, including guanosine monophosphate, adenosine monophosphate, xanthosine monophosphate, and cytidine monophosphate were detected in either or both of SPR and OVN2. In general, the nutritional value of the BSFL meals tested in the present study was influenced by the method of processing.

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Section: Introductionmentioning

confidence: 99%

Nutritional value of black soldier fly (Hermetia illucens) larvae processed by different methods

et al. 2022

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“…Therefore, H. illucens might represent one of the most promising sources for identification of new AMPs. However, discovery, production, and functional investigation of AMPs from H. illucens still remain at a preliminary stage [49]. Recently, putative AMPs sequences have been identified in H. illucens through a combination of transcriptomics and bioinformatics approaches [43].…”

Section: Discussionmentioning

confidence: 99%

Structural and Functional Characterization of a Novel Recombinant Antimicrobial Peptide from Hermetia illucens

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Moretta

Canè

et al. 2021

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Antibiotics are commonly used to treat pathogenic bacteria, but their prolonged use contributes to the development and spread of drug-resistant microorganisms raising the challenge to find new alternative drugs. Antimicrobial peptides (AMPs) are small/medium molecules ranging 10–100 residues synthesized by all living organisms and playing important roles in the defense systems. These features, together with the inability of microorganisms to develop resistance against the majority of AMPs, suggest that these molecules might represent effective alternatives to classical antibiotics. Because of their high biodiversity, with over one million described species, and their ability to live in hostile environments, insects represent the largest source of these molecules. However, production of insect AMPs in native forms is challenging. In this work we investigate a defensin-like antimicrobial peptide identified in the Hermetia illucens insect through a combination of transcriptomics and bioinformatics approaches. The C-15867 AMP was produced by recombinant DNA technology as a glutathione S-transferase (GST) fusion peptide and purified by affinity chromatography. The free peptide was then obtained by thrombin proteolysis and structurally characterized by mass spectrometry and circular dichroism analyses. The antibacterial activity of the C-15867 peptide was evaluated in vivo by determination of the minimum inhibitory concentration (MIC). Finally, crystal violet assays and SEM analyses suggested disruption of the cell membrane architecture and pore formation with leaking of cytosolic material.

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“…In addition to focusing on the interaction between the insect-associated bacteria and B. bassiana, the direct interaction between the insect and entomopathogen was also investigated. By investigating fly immunity, a previous study suggests that insects can encode and secrete various antimicrobial peptides against pathogens (Xia et al, 2021). As for D. antiqua, if this insect could also produce antimicrobial peptides against B. bassiana remains unknown.…”

Section: Discussionmentioning

confidence: 99%

Bacterial Inhibition on Beauveria bassiana Contributes to Microbiota Stability in Delia antiqua

et al. 2021

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Given the multiple roles of associated microbiota in improving animal host fitness in a microbial environment, increasing numbers of researchers have focused on how the associated microbiota keeps stable under complex environmental factors, especially some biological ones. Recent studies show that associated microbiota interacts with pathogenic microbes. However, whether and how the interaction would influence microbiota stability is limitedly investigated. Based on the interaction among Delia antiqua, its associated microbiota, and one pathogen Beauveria bassiana, the associated microbiota's response to the pathogen was determined in this study. Besides, the underlying mechanism for the response was also preliminarily investigated. Results showed that B. bassiana neither infect D. antiqua larvae nor did it colonize inside the associated microbiota, and both the bacterial and fungal microbiota kept stable during the interaction. Further experiments showed that bacterial microbiota almost completely inhibited conidial germination and mycelial growth of B. bassiana during its invasion, while fungal microbiota did not inhibit conidial germination and mycelial growth of B. bassiana. According to the above results, individual dominant bacterial species were isolated, and their inhibition on conidial germination and mycelial growth of B. bassiana was reconfirmed. Thus, these results indicated that bacterial instead of fungal microbiota blocked B. bassiana conidia and stabilized the associated microbiota of D. antiqua larvae during B. bassiana invasion. The findings deepened the understanding of the role of associated microbiota–pathogen microbe interaction in maintaining microbiota stability. They may also contribute to the development of novel biological control agents and pest management strategies.

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Antimicrobial Peptides from Black Soldier Fly (Hermetia illucens) as Potential Antimicrobial Factors Representing an Alternative to Antibiotics in Livestock Farming

Cited by 77 publications

References 124 publications

Nutritional value of black soldier fly (Hermetia illucens) larvae processed by different methods

Nutritional value of black soldier fly (Hermetia illucens) larvae processed by different methods

Structural and Functional Characterization of a Novel Recombinant Antimicrobial Peptide from Hermetia illucens

Bacterial Inhibition on Beauveria bassiana Contributes to Microbiota Stability in Delia antiqua

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