Antimicrobial peptides from amphibians

Xiao, Yao; Liu, Cunbao; Lai, Ren

doi:10.1515/bmc.2011.006

Cited by 19 publications

(13 citation statements)

References 135 publications

(183 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This in turn increases the risk of infection. Because of that, amphibians had evolutionarily developed a series of defense mechanisms, with AMP biosynthesis being one of them [5, 6]. Until now, about 1000 amphibian AMPs have been isolated and examined [7].…”

Section: Introductionmentioning

confidence: 99%

Alanine Scanning Studies of the Antimicrobial Peptide Aurein 1.2

Migoń

Jaśkiewicz

Neubauer

et al. 2018

Probiotics & Antimicro. Prot.

View full text Add to dashboard Cite

Antimicrobial peptides (AMPs) are compounds widely distributed in nature that display activity against a broad spectrum of pathogens. Amphibian skin, as an organ rich in pharmacologically active peptides, appears to be an interesting source of novel AMPs. Aurein 1.2 (GLFDIIKKIAESF-NH 2 ) is a short 13-residue antimicrobial peptide primarily isolated from the skin secretions of Australian bell frogs. In this study, the alanine scan of aurein 1.2 was performed to investigate the effect of each amino acid residue on its biological and physico-chemical properties. The biological studies included determination of minimum inhibitory concentration, activity against biofilm, and inhibitory effect on its formation. Moreover, the hemolytic activity as well as serum stability was determined. The hydrophobicity of peptides and their self-assembly were investigated using reversed-phase chromatography. In addition, their helicity was calculated from circular dichroism spectra. The results not only provided information on structure-activity relationship of aurein 1.2 but also gave insights into design of novel analogs of AMPs in the future. Electronic supplementary material The online version of this article (10.1007/s12602-018-9501-0) contains supplementary material, which is available to authorized users.

show abstract

Section: Introductionmentioning

confidence: 99%

Alanine Scanning Studies of the Antimicrobial Peptide Aurein 1.2

Migoń

Jaśkiewicz

Neubauer

et al. 2018

Probiotics & Antimicro. Prot.

View full text Add to dashboard Cite

show abstract

“…Animal-based medicines, such as insulin, have played a remarkable role in the prevention and treatment of diseases and health care functions. Amphibian skin secretions are rich sources of many bioactive molecules, including antimicrobial peptides (AMPs) [ 42 , 43 , 44 ]. With the overuse of antibiotics in recent years, bacteria developing resistance to these has become a huge problem [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

A Novel Antimicrobial Peptide (Kassinatuerin-3) Isolated from the Skin Secretion of the African Frog, Kassina senegalensis

Wang

Chen

et al. 2020

Biology

View full text Add to dashboard Cite

Amphibian skin secretions are remarkable sources of novel bioactive peptides. Among these, antimicrobial peptides have demonstrated an outstanding efficacy in killing microorganisms via a general membranolytic mechanism, which may offer the prospect of solving specific target-driven antibiotic resistance. Here, the discovery of a novel defensive peptide is described from the skin secretion of the African frog, Kassina senegalensis. Named kassinatuerin-3, it was identified through a combination of “shot-gun” cloning and MS/MS fragmentation sequencing. Subsequently, a synthetic replicate was subjected to biofunctional evaluation. The results indicated that kassinatuerin-3 possessed antimicrobial activity against Gram-positive bacteria but no effect against Gram-negative bacteria. Additionally, it was active in biofilm eradication on S. aureus and MRSA and in the antiproliferation of selected cancer cell lines. Moreover, it had a very mild hemolytic effect, which demonstrated a high therapeutic index for kassinatuerin-3. Collectively, although kassinatuerin-3 did not demonstrate remarkable bioactivities compared with other natural or synthetic antimicrobial peptides (AMPs), it offered a new insight into the design of antimicrobial derivatives.

show abstract

“…The lack of therapeutic options against these pathogens has stimulated research into new bioactive molecules from the biodiversity as a source of more efficient (low toxicity and major potency) mechanisms for infection control (Calderon et al, 2009;Vaara, 2009). The interest in the development of new forms of anti-infective agents such as those based on AMPs from anuran skin as therapeutic agents has been increased (Rinaldi, 2002;Xiao et al, 2011). Thus, they are likely to be active against pathogens and even those that are resistant to conventional drugs.…”

Section: Frog Skin Active Peptides Family: Defence Against Pathogens mentioning

confidence: 99%