Antimicrobial Peptides Derived from the Immune Defense Protein CAP37 Inhibit TLR4 Activation by S100A9

Kasus‐Jacobi, Anne; Land, Craig; Stock, Amanda J.; Washburn, Jennifer L.; Pereira, H. Anne

doi:10.1167/iovs.61.4.16

Cited by 6 publications

(14 citation statements)

References 32 publications

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“…Some AMPs can bind to their targets on the surface of macrophages, and then set off cellular signaling pathway and regulate the secretion of pro-inflammatory factors ( Wang et al, 2011 ; Wei et al, 2015 ; Magrone et al, 2018 ; Zeng et al, 2018 ; Kasus-Jacobi et al, 2020 ; Chai et al, 2021 ). Therefore, the binding of Cath-MH to RAW 264.7 cells were evaluated with flow cytometry.…”

Section: Resultsmentioning

confidence: 99%

The Protective Effects of Cath-MH With Anti-Propionibacterium Acnes and Anti-Inflammation Functions on Acne Vulgaris

Guo

Chai

et al. 2021

Front. Pharmacol.

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Acne vulgaris is a common adolescent skin condition which is mainly caused by Propionibacterium acnes overcolonization and subsequent inflammation. Our previous studies have demonstrated that Cath-MH, an antimicrobial peptide from the skin of the frog Microhyla heymonsivogt, possesses potential antimicrobial, LPS-binding, and anti-septicemic properties. However, its protective effects and potential mechanisms against acne vulgaris are still unclear. In the present study, its anti-P. acnes effects were measured by two-fold broth dilution method, agglutination assay, scanning electron microscopy and confocal laser scanning microscopy experiments. Its treatment potential for acne vulgaris was further evaluated in mice ear inoculated by P. acnes. In addition, the binding ability between Cath-MH and LTA was measured by the Circular Dichroism and antibacterial assay. Moreover, the anti-inflammatory efficiency of Cath-MH was evaluated in LTA- and LPS-induced RAW 264.7 macrophage cells. Cath-MH was found to kill P. acnes with a MIC value of about 1.56 μM by membrane disruption mechanism. It also exhibited agglutination activity against P. acnes. Cath-MH was able to bind LTA as well as LPS, inhibit LTA/LPS-stimulated TLR2/4 expression, and subsequently decreased the inflammatory response in RAW 264.7 cells. As expected, Cath-MH alleviated the formation of edema and the infiltration of inflammatory cells in acne mouse model with concurrent suppression of P. acnes growth and inflammatory cytokines expression in vivo. The potent P. acnes inhibition activity combined with powerful anti-inflammatory effect of Cath-MH indicates its potential as a novel therapeutic option for acne vulgaris.

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Section: Resultsmentioning

confidence: 99%

The Protective Effects of Cath-MH With Anti-Propionibacterium Acnes and Anti-Inflammation Functions on Acne Vulgaris

Guo

Chai

et al. 2021

Front. Pharmacol.

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“…During CA, increased transcriptional expression of S100A9 occurs in the cornea, followed by a release into extracellular space, which enables the inflammatory response to defend against invader microorganisms. The S100A8/A9 heterodimer discloses its pro-inflammation cascade function via RAGE and TLR-4 [ 81 ].…”

Section: Function Of S100 Protein In Host Defense Mechanismmentioning

confidence: 99%

Multifunctional Role of S100 Protein Family in the Immune System: An Update

Singh

Ali

2022

Cells

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S100 is a broad subfamily of low-molecular weight calcium-binding proteins (9–14 kDa) with structural similarity and functional discrepancy. It is required for inflammation and cellular homeostasis, and can work extracellularly, intracellularly, or both. S100 members participate in a variety of activities in a healthy cell, including calcium storage and transport (calcium homeostasis). S100 isoforms have previously been shown to play important roles in immune system disorders such as alarmins (DAMPs), antimicrobial peptides, pro-inflammation stimulators, chemo-attractants, and metal scavengers during an innate immune response. Currently, during the pandemic, it was found that several members of the S100 family are implicated in the pathophysiology of COVID-19. Further, S100 family protein members were proposed to be used as a prognostic marker for COVID-19 infection identification using a nasal swab. In the present review, we compiled the vast majority of recent studies that focused on the multifunctionality of S100 proteins in the complex immune system and its associated activities. Furthermore, we shed light on the numerous molecular approaches and signaling cascades regulated by S100 proteins during immune response. In addition, we discussed the involvement of S100 protein members in abnormal defense systems during the pathogenesis of COVID-19.

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“…Other reports have also shown that LL-37 induced wound healing in the porcine corneal wound model by activation of EGFR signaling via PI3K/Akt and ERK signaling pathways ( Yin and Yu, 2010 ). Cationic antimicrobial protein, CAP37, interacts with TLR4 and promotes corneal re-epithelization in mice ( Kasus-Jacobi et al, 2020 ). Very recently, a dipeptide, isolated from human placental extract has been found to accelerate corneal wound healing both in vitro and in vivo ( Nagata et al, 2015 ).…”

Section: Antimicrobial Peptides and Alternative Therapeutics In Ocular Wound Healingmentioning

confidence: 99%

Alternative Therapeutic Interventions: Antimicrobial Peptides and Small Molecules to Treat Microbial Keratitis

et al. 2021

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Microbial keratitis is a leading cause of blindness worldwide and results in unilateral vision loss in an estimated 2 million people per year. Bacteria and fungus are two main etiological agents that cause corneal ulcers. Although antibiotics and antifungals are commonly used to treat corneal infections, a clear trend with increasing resistance to these antimicrobials is emerging at rapid pace. Extensive research has been carried out to determine alternative therapeutic interventions, and antimicrobial peptides (AMPs) are increasingly recognized for their clinical potential in treating infections. Small molecules targeted against virulence factors of the pathogens and natural compounds are also explored to meet the challenges and growing demand for therapeutic agents. Here we review the potential of AMPs, small molecules, and natural compounds as alternative therapeutic interventions for the treatment of corneal infections to combat antimicrobial resistance. Additionally, we have also discussed about the different formats of drug delivery systems for optimal administration of drugs to treat microbial keratitis.

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Antimicrobial Peptides Derived from the Immune Defense Protein CAP37 Inhibit TLR4 Activation by S100A9

Abstract: Antimicrobial peptides derived from the immune defense protein CAP37 inhibit TLR4 activation by S100A9.

Cited by 6 publications

References 32 publications

The Protective Effects of Cath-MH With Anti-Propionibacterium Acnes and Anti-Inflammation Functions on Acne Vulgaris

The Protective Effects of Cath-MH With Anti-Propionibacterium Acnes and Anti-Inflammation Functions on Acne Vulgaris

Multifunctional Role of S100 Protein Family in the Immune System: An Update

Alternative Therapeutic Interventions: Antimicrobial Peptides and Small Molecules to Treat Microbial Keratitis

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