Antimicrobial peptides derived from goose egg white lysozyme

Thammasirirak, Sompong; Pukcothanung, Yuwatida; Preecharram, Sutthidech; Daduang, Sakda; Patramanon, Rina; Fukamizo, Tamo; Araki, Toshimitsu

doi:10.1016/j.cbpc.2009.08.009

Cited by 32 publications

(28 citation statements)

References 45 publications

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“…These features facilitate its affinity binding to the negatively charged bacterial cell membrane surface. 9 The results further support the previous conclusion that most antimicrobial peptides are hydrophobic and less than 10 kDa in molecular weight, with an overall net positive charge. 1 Searches in the APD and NCBI databases found no match, suggesting that it is a novel peptide, which we have named "Apep10".…”

Section: ■ Results and Discussionsupporting

confidence: 88%

New Cationic Antimicrobial Peptide Screened from Boiled-Dried Anchovies by Immobilized Bacterial Membrane Liposome Chromatography

Tang

Zhang

Wang

et al. 2014

J. Agric. Food Chem.

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An efficient immobilized bacterial membrane liposome chromatography method was used to screen potential antimicrobial peptides from boiled-dried anchovies. A novel cationic antimicrobial peptide (Apep10) was successfully isolated by one-step chromatography. The sequence of Apep10 was identified as GLARCLAGTL by matrix-assisted laser desorption/ionization quadrupole time-of-flight tandem mass spectrometry (MALDI-Q-TOF MS). The antimicrobial activity assessment indicated that Apep10 inhibited the growth of the reference bacteria (Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus, Bacillus subtilis, and Streptococcus pneumoniae), with minimal inhibitory concentration (MIC) values ranging from 8 to 64 μg/mL. Almost no cytotoxicity against mouse erythrocytes was observed at concentrations below 20 μg/mL. Nucleotide leakage induced by Apep10 showed that the peptide exhibited permeable activity on the cytoplasmic membrane. Alterations in morphology were observed by scanning electronic microscopy (SEM). Membrane disruption was confirmed by confocal laser scanning microscopy (CLSM) with propidium iodide (PI). The results demonstrate that immobilized bacterial membrane liposome chromatography is a straightforward technique for screening unknown antimicrobial peptides with cell-membrane-interacting activities from boiled-dried anchovies.

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Section: ■ Results and Discussionsupporting

confidence: 88%

New Cationic Antimicrobial Peptide Screened from Boiled-Dried Anchovies by Immobilized Bacterial Membrane Liposome Chromatography

Tang

Zhang

Wang

et al. 2014

J. Agric. Food Chem.

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“…To polish the antimicrobial activity of lysozyme fraction, hydrolysis of donkey milk was carried out in two steps [14]. Initially, donkey milk was acidified with HCl to a pH 3.5, and then incubated with 2 mg/ml pepsin (Sigma) at 37°C for 2h.…”

Section: Enzymatic Hydrolysis Of Lysozyme In Donkey Milkmentioning

confidence: 99%

Antimicrobial Activity of Donkey Milk against Dermatomycotic Fungi and Foodborne Bacteria

Koutb¹,

Khider²,

Ali³

et al. 2016

IJBMR

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Abstract:The antimicrobial activity of donkey milk was examined against 3 bacterial and 3 fungal strains selected on the basis of their relevance as human pathogens. All samples of donkey milk exhibited antimicrobial activity against dermatomycotic fungi and foodborne pathogen bacteria. The highest antimicrobial activity was recorded against Trichophyton mentagrophytes and T. rubrum with minimal lethal concentration of 32 mg/ml. In respect to foodborne pathogenic bacteria, Gram-positive bacteria (Bacillus cereus and Staphylococcus aureus) were more sensitive to donkey milk than Gram-negative bacteria (E. coli) with minimal lethal concentrations of 32, 64 and 128 mg/ml respectively. Donkey milk kept about 60 to 62% of its antimicrobial activity against B. cereus and S. aurous respectively, after digestion with pepsin (2 mg/ml), suggesting that the fatty acids of donkey milk has the highest antimicrobial effect. While the antimicrobial activity against dermatomycotic fungi and Gram-negative bacteria not affected after digestion with pepsin. To explain the antifungal capability of donkey milk against dermatomycotic fungi, fatty acids were analyzed by gas chromatography. Fatty acids analysis indicated that the major constituents in donkey milk lipid are oleic (25.4%), palmitic (23.75%), linolenic (20.04%), arachidic (3.58%) and stearic (3.26%), which have antimicrobial activity. Finally, on the basis of results obtained in the current study, the antimicrobial activity of donkey milk against Staphylococcus aureus and dermatomycotic fungi, specially T. mentagrophytes and T. rubrum which frequently cause acute or chronic inflammatory tinea corporis; may be considered as a valuable natural product with novel functional protection properties in cosmetics and pharmaceutical industries.

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“…Evidence for a potential function for these lysozymes come from the observations of significant antibacterial activity in enzymatically-inactivated lysozymes17 and for proteolytic fragments, or synthetic peptides, derived from lysozyme proteins18. In these cases, the membrane-penetrating capacity of the lysozyme or lysozyme-derived fragment mediates the antibacterial action.…”

Section: Discussionmentioning

confidence: 99%

“…Ibrahim et al 17 also gave evidence for retained antibacterial activity for lysozymes catalytically inactivated by substitution of an active site residue. In addition, previous reports have shown antibacterial activity for proteolytic fragments of both lysozymes18.…”

mentioning

confidence: 87%

Multiple specialised goose-type lysozymes potentially compensate for an exceptional lack of chicken-type lysozymes in Atlantic cod

Seppola

Bakkemo

Mikkelsen

et al. 2016

Sci Rep

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Previous analyses of the Atlantic cod genome showed unique combinations of lacking and expanded number of genes for the immune system. The present study examined lysozyme activity, lysozyme gene distribution and expression in cod. Enzymatic assays employing specific bacterial lysozyme inhibitors provided evidence for presence of g-type, but unexpectedly not for c-type lysozyme activity. Database homology searches failed to identify any c-type lysozyme gene in the cod genome or in expressed sequence tags from cod. In contrast, we identified four g-type lysozyme genes (LygF1a-d) constitutively expressed, although differentially, in all cod organs examined. The active site glutamate residue is replaced by alanine in LygF1a, thus making it enzymatic inactive, while LygF1d was found in two active site variants carrying alanine or glutamate, respectively. In vitro and in vivo infection by the intracellular bacterium Francisella noatunensis gave a significantly reduced LygF1a and b expression but increased expression of the LygF1c and d genes as did also the interferon gamma (IFNγ) cytokine. These results demonstrate a lack of c-type lysozyme that is unprecedented among vertebrates. Our results further indicate that serial gene duplications have produced multiple differentially regulated cod g-type lysozymes with specialised functions potentially compensating for the lack of c-type lysozymes.

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Antimicrobial peptides derived from goose egg white lysozyme

Cited by 32 publications

References 45 publications

New Cationic Antimicrobial Peptide Screened from Boiled-Dried Anchovies by Immobilized Bacterial Membrane Liposome Chromatography

New Cationic Antimicrobial Peptide Screened from Boiled-Dried Anchovies by Immobilized Bacterial Membrane Liposome Chromatography

Antimicrobial Activity of Donkey Milk against Dermatomycotic Fungi and Foodborne Bacteria

Multiple specialised goose-type lysozymes potentially compensate for an exceptional lack of chicken-type lysozymes in Atlantic cod

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