Antimicrobial peptide production and plant-based expression systems for medical and agricultural biotechnology

Holásková, Edita; Galuszka, Petr; Frébort, Ivo; Öz, Mehmet Tufan

doi:10.1016/j.biotechadv.2015.03.007

Cited by 114 publications

(94 citation statements)

References 207 publications

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“…Heterologous expression of AMPs in various plantbased expression platforms includes plant cell suspensions and hairy root culture; field-grown transgenic plants provide various suitable alternative platforms for the production of AMPs [18], and the development of new approaches in crop protection [19]. These systems have emerged as promising hosts for AMP production because of their advantages over the currently applied expression systems based on bacteria or yeast [20,21].…”

Section: Introductionmentioning

confidence: 99%

“…These systems have emerged as promising hosts for AMP production because of their advantages over the currently applied expression systems based on bacteria or yeast [20,21]. Plants could successfully perform appropriate glycosylation, folding and disulphide bond formation of recombinant AMPs that are often essential for their biological activity [18]. Plant-based systems offer more advantages towards product yield, quality, homogeneity and safety compared to other systems [22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

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Recombinant expression of LFchimera antimicrobial peptide in a plant-based expression system and its antimicrobial activity against clinical and phytopathogenic bacteria

Chahardoli

Fazeli

Niazi‎

et al. 2018

Biotechnology & Biotechnological Equipment

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Antimicrobial peptides (AMPs) with their broad and selective antimicrobial activity and lowered risk of resistance development in microbial populations are promining as a new alternative candidate to conventional antibiotics. Plant-based expression systems can be employed as cost-effective and high scale-up capacity production hosts for recombinant expression of AMPs. LFchimera is a chimerical peptide containing LFcin and LFampin antimicrobial peptides of bovine lactoferrin, and it has stronger bactericidal activity. Here, the LFchimera sequence was codon-optimized for tobacco and fused with endoplasmic reticulum retention signals along with a CaMV 35S promoter and then transferred by agrobacterium-mediated transformation. The integration and expression of the transgene in tobacco plants were confirmed by polymerase chain reaction (PCR) and RT-PCR, respectively. Recombinant production of the peptide was confirmed by sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE), and peptide functional activity was confirmed by the antibacterial evaluation of total protein extracts against various clinical and phytopathogenic bacteria. Finally, LFchimera peptide was partially purified using an affinity column, and the antibacterial activity was shown. Taken together, these results confirmed that tobacco can be utilized for the recombinant production of antimicrobial peptides such as LFchimera.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Recombinant expression of LFchimera antimicrobial peptide in a plant-based expression system and its antimicrobial activity against clinical and phytopathogenic bacteria

Chahardoli

Fazeli

Niazi‎

et al. 2018

Biotechnology & Biotechnological Equipment

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show abstract

“…AMPs, also called peptide antibiotics [24] are a growing class of low molecular mass oligopeptides found in many organisms with a broad range of antimicrobial activity against viruses, fungi and bacteria. Unlike antibiotics, AMPs targets lipopolysaccharides in the microbial cell membrane and induce rapid killing of pathogens.…”

Section: Recombinant Protein Production In Plant Chloroplastsmentioning

confidence: 99%

Expression and functional evaluation of biopharmaceuticals made in plant chloroplasts

Zhang

Shanmugaraj

Daniell

2017

Current Opinion in Chemical Biology

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After approval of the first plant-made biopharmaceutical by FDA for human use, many protein drugs are now in clinical development. Within the last decade, significant advances have been made in expression of heterologous complex/large proteins in chloroplasts of edible plants using codon optimized human or viral genes. Furthermore, advances in quantification enable determination of in-planta drug dosage. Oral delivery of plastid-made biopharmaceuticals (PMB) is affordable because it eliminates prohibitively expensive fermentation, purification processes addressing major challenges of short shelf-life after cold storage. In this review, we discuss recent advances in PMBs against metabolic, inherited or infectious diseases, and also mechanisms of post-translational modifications (PTM) in order to increase our understanding of functional PMBs.

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“…Antimicrobial peptides and proteins were identified as antibacterial agents as they affect bacterial critical membrane functions [9] This opens for discovering new alternatives to fight these microorganisms and may enable advances in medical, veterinarian and agriculture areas [10] Interestingly, emerging evidence has demonstrated additional functions for these molecules such as chemotactic and immunomodulatory activities [9].…”

Section: Antimicrobial Peptidesmentioning

confidence: 99%

Defensins and Bioinformatics: In Silico Approaches for Novel Therapeutic Antimicrobial Peptides

Castro¹,

Sathler²

2016

Ely J Micro

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Defensins are antimicrobial proteins that help the immune system of animals and plants on avoiding pathogens and maintaining the body integrity. According to recent literature, humanity is in need of a new golden age of antibiotics as resistant bacterial strains are turning simple infections into lethal weapons. Therefore, defensins may help as biotechnological prototypes with an antibacterial profile to generate new antimicrobial drugs. In this work we present the in silico perspective of the molecular modeling, a subarea of Bioinformatic, as a tool to be used in comparative analysis of defensins to identify important molecular features that modulate their biological activity profile. Parameters such as electrostatic potential map, RMS and volume can be used in the structural analysis of mutants, helping to understand the defensins mechanism of action. Considering the absence of more effective, selective and low-toxicity antimicrobial treatments, this knowledge may help in designing these and other new molecules also contributing to the beginning of this new golden age of antibiotics so currently in need.

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Antimicrobial peptide production and plant-based expression systems for medical and agricultural biotechnology

Cited by 114 publications

References 207 publications

Recombinant expression of LFchimera antimicrobial peptide in a plant-based expression system and its antimicrobial activity against clinical and phytopathogenic bacteria

Recombinant expression of LFchimera antimicrobial peptide in a plant-based expression system and its antimicrobial activity against clinical and phytopathogenic bacteria

Expression and functional evaluation of biopharmaceuticals made in plant chloroplasts

Defensins and Bioinformatics: In Silico Approaches for Novel Therapeutic Antimicrobial Peptides

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