Antimicrobial Peptide-Loaded Pectolite Nanorods for Enhancing Wound-Healing and Biocidal Activity of Titanium

Abstract

Titanium is widely utilized for manufacturing medical implants due to its inherent mechanical strength and biocompatibility. Recent studies have focused on developing coatings to impart unique properties to Ti implants, such as antimicrobial behavior, enhanced cell adhesion, and osteointegration. Ca- and Si-based ceramic (CS) coatings can enhance bone integration through the release of Ca and Si ions. However, high degradation rates of CS ceramics create a basic environment that reduces cell viability. Polymeric or protein-based coatings may be employed to modulate CS degradation. However, it is challenging to ensure coating stability over extended periods of time without compromising biocompatibility. In this study, we employed a fluorous-cured collagen shell as a drug-loadable scaffold around CS nanorod coatings on Ti implants. Fluorous-cured collagen coatings have enhanced mechanical and enzymatic stability and are able to regulate the release of Ca and Si ions. Furthermore, the collagen scaffold was loaded with antimicrobial peptides to impart antimicrobial activity while promoting cell adhesion. These multifunctional collagen coatings simultaneously regulate the degradation of CS ceramics and enhance antimicrobial activity, while maintaining biocompatibility.

pH-Responsive ECM Coating on Ti Implants for Antibiosis in Reinfected Models

“…Co., Ltd.) were spin-coated on HSF samples at 300 rpm for 50 s. The spin-coated samples thermally treated in perfluoroperhydrophenanthrene (PFHP, Sigma-Aldrich) for 15 min at 180 °C, washed with perfluorohexane (Sigma-Aldrich), and dried with a stream of a N 2 gas. 10 The samples were abbreviated as HC and HCAx. HC represents samples with pure Col-I and x represents the weight ratio of AMP (HHC-36) in Col-I.…”

Multifunctional Coatings of Titanium Implants Toward Promoting Osseointegration and Preventing Infection: Recent Developments

“…The coating did inhibit bacterial infection in the early stage and enhanced osseointegration in the later stage. Zhang et al prepared Ca- and Si-based ceramic (CS) nanorod coatings on Ti using MAO technique, and loaded AMP onto CS coatings with the aid of fluorous-cured collagen scaffolds ( Zhang et al, 2021 ). The release of Ca 2+ and Si 2+ enhanced osseointegration and the collagen scaffold loaded with AMP had a good antimicrobial effect while promoting cell adhesion.…”

Application of Antimicrobial Peptides on Biomedical Implants: Three Ways to Pursue Peptide Coatings

“…Afterwards, this collagen scaffold was loaded with the antimicrobial peptide HHC-36 (KRWWKWWRR) to impart antimicrobial activity while promoting cell adhesion. Notably, this multifunctional approach allowed regulation of the rate of nanorod degradation, and also enhanced cytocompatibility of the implant making it a promising tool for future use in coatings for bone regeneration [58].…”