Antimicrobial peptide, hdMolluscidin, purified from the gill of the abalone, Haliotis discus

Seo, Jung-Kil; Go, Hye‐Jin; Kim, Chan‐Hee; Nam, Bo‐Hye; Park, Nam Gyu

doi:10.1016/j.fsi.2016.03.150

Cited by 20 publications

(7 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, an antimicrobial peptide expressed only in gills has been discovered in the abalone Haliotis discus [51]. Since the gills may be important in the resistance to V. harveyi infection, future work comparing potential antimicrobial or anti-adherent activity in the gills of abalone from the two populations may help to identify the mechanisms by which abalone from Saint-Malo have enhanced resistance against V. harveyi.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Immune priming and portal of entry effectors improve response to vibrio infection in a resistant population of the European abalone

Dubief

Nunes

Basuyaux³

et al. 2017

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Since 1997, populations of the European abalone Haliotis tuberculata suffer mass mortalities attributed to the bacterium Vibrio harveyi. These mortalities occur at the spawning season, when the abalone immune system is depressed, and when temperatures exceed 17 °C, leading to favorable conditions for V. harveyi proliferation. In order to identify mechanisms of disease resistance, experimental successive infections were carried out on two geographically distinct Brittany populations: one that has suffered recurrent mortalities (Saint-Malo) and one that has not been impacted by the disease (Molène). Furthermore, abalone surviving these two successive bacterial challenges and uninfected abalone were used for several post-infection analyses. The Saint-Malo population was found to be resistant to V. harveyi infection, with a survival rate of 95% compared to 51% for Molène. While in vitro quantification of phagocytosis by flow cytometry showed strong inhibition following the first infection, no inhibition of phagocytosis was observed following the second infection for Saint-Malo, suggesting an immune priming effect. Moreover, assays of phagocytosis of GFP-labelled V. harveyi performed two months post-infection show an inhibition of phagocytosis by extracellular products of V. harveyi for uninfected abalone, while no effect was observed for previously infected abalone from Saint-Malo, suggesting that the effects of immune priming may last upwards of two months. Detection of V. harveyi by qPCR showed that a significantly greater number of abalone from the susceptible population were positive for V. harveyi in the gills, indicating that portal of entry effectors may play a role in resistance to the disease. Collectively, these results suggest a potential synergistic effect of gills and hemolymph in the resistance of H. tuberculata against V. harveyi with an important involvement of the gills, the portal of entry of the bacteria.

show abstract

Section: Accepted Manuscriptmentioning

confidence: 99%

Immune priming and portal of entry effectors improve response to vibrio infection in a resistant population of the European abalone

Dubief

Nunes

Basuyaux³

et al. 2017

Fish & Shellfish Immunology

View full text Add to dashboard Cite

show abstract

“…An ultrasensitive radial diffusion assay was adopted for monitoring antimicrobial activity during the purification steps and for testing synthetic peptides, as described previously (Seo et al, 2016 (Table 1). Briefly, microbial strains were pre-cultured overnight in tryptic soy broth (TSB) at the appropriate temperatures, 25 °C for fish pathogens and 37 °C for the others.…”

Section: Antimicrobial Activity Assaymentioning

confidence: 99%

Identification of a novel antimicrobial peptide from the sea star Patiria pectinifera

Kim

et al. 2018

Developmental & Comparative Immunology

Self Cite

View full text Add to dashboard Cite

Antimicrobial peptides (AMPs) are components of innate immunity found in many forms of life. However, there have been no reports of AMPs in sea star (Phylum Echinodermata). Here we report the isolation and characterization of a novel antimicrobial peptide from the coelomic epithelium extract of the sea star Patiria pectinifera. The isolated peptide comprises 38 amino acid residues, is cationic (pI 9.2), has four cysteine residues that form two disulfide bonds (C1-C3 and C2-C4), is amidated at the C-terminus, and is designated P. pectinifera cysteine-rich antimicrobial peptide (PpCrAMP). Synthetic PpCrAMP identical to the native peptide exhibited the most potent antimicrobial activity compared to analogs with different disulfide bond configurations. Expression analysis of PpCrAMP precursor transcripts revealed constitutive expression in the coelomic epithelium and tube feet of P. pectinifera. Analysis of genomic DNA and cDNA encoding the PpCrAMP precursor protein revealed that an intron splits the coding region of the mature peptide into a positively charged N-terminal domain and a C-terminal domain harboring four cysteine residues and a glycine for C-terminal amidation. No significant homology with other known AMPs was observed, while orthologs of PpCrAMP were found in other echinoderm species. These findings indicate that PpCrAMP is the prototype of a family a novel cysteine-rich AMPs that participate in mechanisms of innate immunity in echinoderms. Furthermore, the discovery of PpCrAMP may lead to the identification of related AMPs in vertebrates and protostome invertebrates.

show abstract

“…The latter involves hemocyanin, the respiratory protein of the mollusc, 15 which has been found to possess the promising antibacterial activity in the host immunity when infection occurs. 16,17 The antibacterial activity of hemocyanin has been widely identified in numerous studies on invertebrates, including molluscs [18][19][20][21] and arthropods. [22][23][24][25][26] Hemocyanin is structurally related to Phenoloxidase 27,28 and acquires stronger antibacterial activity when treated with sodium dodecyl sulphate 28 or after activation by microbial proteases.…”

Section: Compounds With Antibacterial Activity Isolated From Abalonementioning

confidence: 99%

Study on novel antibacterial and antiviral compounds from abalone as an important marine mollusc

Valtchev

Sairi

et al. 2018

JAMB

View full text Add to dashboard Cite

Infectious diseases caused by bacteria and viruses are the most common cause of death in developing countries. The acquisition of antibiotic resistance and emergence of new pathogens require the development of new antibacterial and antiviral compounds with different mechanism of action. Abalone, a commercially important marine mollusc, has recently been found to possess arrange of compounds with potent antibacterial and antiviral activity. Some of the active compounds identified in abalones, such as antimicrobial peptides (AMP) and hemocyanin, demonstrated diverse mechanisms of action against a wide variety of human pathogens. These promising lead compounds may provide a development platform for novel antibacterial and antiviral drugs in the near future.

show abstract

Antimicrobial peptide, hdMolluscidin, purified from the gill of the abalone, Haliotis discus

Cited by 20 publications

References 18 publications

Immune priming and portal of entry effectors improve response to vibrio infection in a resistant population of the European abalone

Immune priming and portal of entry effectors improve response to vibrio infection in a resistant population of the European abalone

Identification of a novel antimicrobial peptide from the sea star Patiria pectinifera

Study on novel antibacterial and antiviral compounds from abalone as an important marine mollusc

Contact Info

Product

Resources

About