Antimicrobial Peptide GH12 Prevents Dental Caries by Regulating Dental Plaque Microbiota

Jiang, Wentao; Wang, Yufei; Luo, Jingjie; Chen, Xiangshu; Zeng, Yuhan; Li, Xinwei; Feng, Zhongxin; Zhang, Linglin

doi:10.1128/aem.00527-20

Cited by 24 publications

(27 citation statements)

References 48 publications

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“…[ 149 ] An in vivo study reported that GH12, a cationic amphipathic α ‐helical AMP, reduced the production of exopolysaccharides by S. mutans biofilms and decreased the acidity of the microenvironment. [ 153 ] Peptide polyphemusin I is another natural AMP isolated from horseshoe crabs that contain 17 to 18 amino acids. [ 154 ] In vitro and in vivo results confirmed the antimicrobial properties of the peptide polyphemusin I.…”

Section: Gene and Protein Deliverymentioning

confidence: 99%

Drug Delivery (Nano)Platforms for Oral and Dental Applications: Tissue Regeneration, Infection Control, and Cancer Management

et al. 2021

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The oral cavity and oropharynx are complex environments that are susceptible to physical, chemical, and microbiological insults. They are also common sites for pathological and cancerous changes. The effectiveness of conventional locally-administered medications against diseases affecting these oral milieus may be compromised by constant salivary flow. For systemically-administered medications, drug resistance and adverse side-effects are issues that need to be resolved. New strategies for drug delivery have been investigated over the last decade to overcome these obstacles. Synthesis of nanoparticle-containing agents that promote healing represents a quantum leap in ensuring safe, efficient drug delivery to the affected tissues. Micro/nanoencapsulants with unique structures and properties function as more favorable drug-release platforms than conventional treatment approaches. The present review provides an overview of newly-developed nanocarriers and discusses their potential applications and limitations in various fields of dentistry and oral medicine.

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Section: Gene and Protein Deliverymentioning

confidence: 99%

Drug Delivery (Nano)Platforms for Oral and Dental Applications: Tissue Regeneration, Infection Control, and Cancer Management

et al. 2021

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“…This microecology not only provides a great haven for the reproduction of caries pathogens and the fermentation of an acidic environment ( Filoche et al, 2010 ) but also resists attack from foreign substances, including antibiotics and antibacterial agents ( Pizzolato-Cezar et al, 2019 ). Many agents have an antibacterial effect on cariogenic pathogens in suspension ( Jiang et al, 2020 ; Zhang et al, 2021 ), but it is extremely difficult for these agents to penetrate into biofilm and attack the pathogens in the biofilm. This is a major challenge for the clinical applications of these agents for dental caries management.…”

Section: Introductionmentioning

confidence: 99%

A New pH-Responsive Nano Micelle for Enhancing the Effect of a Hydrophobic Bactericidal Agent on Mature Streptococcus mutans Biofilm

Zhang

2021

Front. Microbiol.

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The bactericidal effect on biofilm is the main challenge currently faced by antibacterial agents. Nanoscale drug-delivery materials can enhance biofilm penetrability and drug bioavailability, and have significant applications in the biomedical field. Dental caries is a typical biofilm-related disease, and the acidification of biofilm pH is closely related to the development of dental caries. In this study, a pH-responsive core-shell nano micelle (mPEG-b-PDPA) capable of loading hydrophobic antibacterial agents was synthesized and characterized, including its ability to deliver antibacterial agents within an acidic biofilm. The molecular structure of this diblock copolymer was determined by hydrogen-1 nuclear magnetic resonance (1H-NMR) and gel permeation chromatography (GPC). The characters of the micelles were studied by dynamic light scattering (DLS), TEM, pH titration, and drug release detection. It was found that the hydrophilic micelles could deliver bedaquiline, a hydrophobic antibacterial agent on S. mutans, in acidic environments and in mature biofilm. No cytotoxic effect on the periodontal cells was detected within 48 h. This pH-responsive micelle, being able to load hydrophobic antibacterial agent, has good clinical application potential in preventing dental caries.

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“…Together, our results indicate that OMVs derived from certain bacteria may be a viable adjunct therapy to enhance the effectiveness of antibiotics against bacterial biofilms. They could also be used in conjunction with other new antibiofilm therapeutics, such as recently described antimicrobial peptides D-CONGA and GH-12 that display antibiofilm activities [51,52].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Streptococcus mutans biofilms with bacterial-derived outer membrane vesicles

et al. 2021

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Background Biofilms are microbial communities surrounded by a self-produced extracellular matrix which protects them from environmental stress. Bacteria within biofilms are 10- to 1000-fold more resistant to antibiotics, making it challenging but imperative to develop new therapeutics that can disperse biofilms and eradicate infection. Gram-negative bacteria produce outer membrane vesicles (OMV) that play critical roles in communication, genetic exchange, cargo delivery, and pathogenesis. We have previously shown that OMVs derived from Burkholderia thailandensis inhibit the growth of drug-sensitive and drug-resistant bacteria and fungi. Results Here, we examine the antibiofilm activity of Burkholderia thailandensis OMVs against the oral biofilm-forming pathogen Streptococcus mutans. We demonstrate that OMV treatment reduces biofilm biomass, biofilm integrity, and bacterial cell viability. Both heat-labile and heat-stable components, including 4-hydroxy-3-methyl-2-(2-non-enyl)-quinoline and long-chain rhamnolipid, contribute to the antibiofilm activity of OMVs. When OMVs are co-administered with gentamicin, the efficacy of the antibiotic against S. mutans biofilms is enhanced. Conclusion These studies indicate that bacterial-derived OMVs are highly effective biological nanoparticles that can inhibit and potentially eradicate biofilms.

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Antimicrobial Peptide GH12 Prevents Dental Caries by Regulating Dental Plaque Microbiota

Cited by 24 publications

References 48 publications

Drug Delivery (Nano)Platforms for Oral and Dental Applications: Tissue Regeneration, Infection Control, and Cancer Management

Drug Delivery (Nano)Platforms for Oral and Dental Applications: Tissue Regeneration, Infection Control, and Cancer Management

A New pH-Responsive Nano Micelle for Enhancing the Effect of a Hydrophobic Bactericidal Agent on Mature Streptococcus mutans Biofilm

Inhibition of Streptococcus mutans biofilms with bacterial-derived outer membrane vesicles

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