Antimicrobial peptide gene cecropin-2 and defensin respond to peptidoglycan infection in the female adult of oriental fruit fly, Bactrocera dorsalis (Hendel)

Liu, Shi-Huo; Wei, Dong; Yuan, Guangjie; Jiang, Hong‐Bo; Dou, Wei; Wang, Jinjun

doi:10.1016/j.cbpb.2017.01.004

Cited by 17 publications

(17 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the spatiotemporal expression profiles of BdPho , samples at different developmental stages and different tissues of adult were collected as described previously (Liu et al 2017 ).…”

Section: Methodsmentioning

confidence: 99%

“…Before cDNA synthesis, the contaminating genomic DNA of each RNA sample was digested with DNase I (Promega, Madison, WI). First-strand cDNA was synthesized as described previously (Liu et al 2017 ).…”

Section: Methodsmentioning

confidence: 99%

“…The specific primers used for amplifying open reading frame of BdPho (forward, 5′-TTTCACACACCTCAGTAAACTTTCT-3′; reverse, 5′-TTATTCCCCTCTCTTCAGTCGT-3′) were synthesized by Invitrogen in Shanghai, China. All PCRs were performed with 25 μL reaction mixtures as described previously (Liu et al 2017 ). PCR parameters were: 94 °C for 3 min, followed by 35 cycles of 94 °C for 30 s, anneal of 58 °C for 30 s, and extension of 72 °C for 1 min, final extension of 72 °C for 7 min.…”

Section: Methodsmentioning

confidence: 99%

“…Thereafter, more and more different defensins have been identified from various insects. Insect defensins are widely investigated in the orders of Diptera, Coleoptera, Lepidoptera, Hemiptera, and Hymenoptera (Liu et al 2017 ; Tian et al 2010 ; Ueda et al 2005 ; Wang et al 2013b ; Wen et al 2009 ). Note worthily, defensin is also present in the ancient order of Odonata (Bulet et al 1992 ), suggesting that insect defensins may derive from a common ancestor gene.…”

Section: Introductionmentioning

confidence: 99%

“…Dipteran insects are considered as microorganism-rich and expected to be abundant in AMPs. To date, only five groups of AMPs have been identified in this species, including cecropins, defensins, attacins, bactrocerin, and diptericins (Dang et al 2009 ; Jiang et al 2014 ; Liao et al 2015 ; Liu et al 2017 ). To better understand the adaptation of B. dorsalis to different environments and also provide more natural molecules with potential for designing effective antibiotics applied in medicine and agriculture (Yi et al 2014 ), it is worthy to identify more AMPs and analyze the immune response challenged by the immune triggers in B. dorsalis .…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Antimicrobial peptide gene BdPho responds to peptidoglycan infection and mating stimulation in oriental fruit fly, Bactrocera dorsalis (Hendel)

Liu

Yang

et al. 2018

AMB Expr

Self Cite

View full text Add to dashboard Cite

Phormicins belong to defensin family, which are important antimicrobial peptides (AMPs) in insects. These AMPs are inducible upon challenging by immune triggers. In the present study, we identified the cDNA of a phormicin gene (BdPho) in the oriental fruit fly, Bactrocera dorsalis (Hendel), a ruinous agricultural pest causing great economic losses to fruits and vegetables. The cDNA of BdPho contains a 282 bp open reading frame encoding 93 amino acid residues, and the predicted molecular weight and isoelectric point of BdPho peptide were 9.83 kDa and 7.54, respectively. Quantitative real-time PCR analyses showed that the transcription level of BdPho was the highest in adult during different developmental stages and was the highest in abdomen among adult tagmata. Moreover, BdPho was highly expressed in fat body among different tissues, both in female and male adult. The mRNA level of BdPho was significantly up-regulated to 7.46- and 14.53-fold at 3 and 6 h after the insects were challenged with peptidoglycans from Escherichia coli (PGN-EB), respectively, suggesting its antimicrobial activity against Gram-negative microorganisms. Furthermore, the expression level of BdPho was significantly up-regulated to 3.83-fold after mating, suggesting that female adults might enhance their immunity by up-regulating the expression level of BdPho during mating. These results firstly describe the basic properties of the phormicin gene from B. dorsalis, and lay the foundation for investigating functional properties of AMPs and exploring the molecular mechanisms in the immune system.Electronic supplementary materialThe online version of this article (10.1186/s13568-017-0533-8) contains supplementary material, which is available to authorized users.

show abstract

“…For the spatiotemporal expression profiles of BdPho , samples at different developmental stages and different tissues of adult were collected as described previously (Liu et al 2017 ).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Antimicrobial peptide gene BdPho responds to peptidoglycan infection and mating stimulation in oriental fruit fly, Bactrocera dorsalis (Hendel)

Liu

Yang

et al. 2018

AMB Expr

Self Cite

View full text Add to dashboard Cite

show abstract

The immune response of Locusta migratoria manilensis at different times of infection with Paranosema locustae

Liu,

Wei,

et al. 2023

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

Paranosema locustae is an entomopathogenic microsporidia with promising potential for controlling agricultural pests, including Locusta migratoria manilensis. However, it has the disadvantage of having a slow insecticidal rate, and how P. locustae infection impacts the host immune response is currently unknown. The present study investigated the effect of P. locustae on the natural immune response of L. migratoria and the activities of enzymes that protect against oxidative stress. Infection with P. locustae increased the hemocytes and nodulation number of L. migratoria at the initial stage of infection. The hemocyte‐mediated modulation of immune response was also affected by a decrease in the number of hemocytes 12 days postinfection. Superoxide dismutase activity in locusts increased in the early stages of infection but decreased in the later stages, whereas the activities of peroxidase (POD) and catalase (CAT) showed opposite trends may be due to their different mechanisms of action. Furthermore, the transcription levels of mRNA of antimicrobial peptide‐related genes and phenoloxidase activity in hemolymph in L. migratoria were suppressed within 15 days of P. locustae infection. Overall, our data suggest that P. locustae create a conducive environment for its own proliferation in the host by disrupting the immune defense against it. These findings provide useful information for the potential application of P. locustae as a biocontrol agent.

show abstract

Potential targets for controlling Bactrocera dorsalis using cuticle‐ and hormone‐related genes revealed by a developmental transcriptome analysis

Liu

Xia

Zhang

et al. 2020

Pest Management Science

Self Cite

View full text Add to dashboard Cite

BACKGROUND: The oriental fruit fly, Bactrocera dorsalis (Hendel), is an important agricultural pest and has developed resistance to many insecticides. To investigate vital genes participating in metamorphosis for development of additional control strategies, a comprehensive transcriptome analysis covering ten developmental stages of B. dorsalis was performed.RESULTS: There were 2132, 952, 1062, 2301 and 1333 differentially expressed genes identified during hatching, 1st-instar larval molting, 2nd-instar larval molting, pupariation and emergence, respectively. Further expression analyses indicated that genes in hormone-(20-hydroxyecdysone and juvenile hormone) and cuticle-(chitin and cuticle protein) related pathways were essential for metamorphosis in B. dorsalis. Among chitinase (Cht) genes, BdCht-5, −8 and −10 were differentially expressed during larval-larval, larval-pupal and pupal-adult moltings. However, BdCht7 was differentially expressed during egg-larval and larval-larval moltings. Knockdown of BdCht7 at the 1st-instar larval stage disrupted normal development of larvae and was lethal to B. dorsalis. Among cuticle protein (CP) genes, 15 genes (BdCPLCG-1, and BdRR1-41) were differentially expressed during four of five types of moltings. Among hormone-relative genes, BdJHBP-4, −9 and −13 were differentially expressed during all five types of moltings, whereas BdJHBP-5, −12 and BdHR4 were differentially expressed during four of five types of moltings.CONCLUSION: This study reveals critical genes involved in development and metamorphosis of B. dorsaslis, and BdCht7 is dispensable for larval survival. It also provides comprehensive transcriptome information for finding more molecular targets to control this pest.

show abstract

Antimicrobial peptide gene cecropin-2 and defensin respond to peptidoglycan infection in the female adult of oriental fruit fly, Bactrocera dorsalis (Hendel)

Cited by 17 publications

References 38 publications

Antimicrobial peptide gene BdPho responds to peptidoglycan infection and mating stimulation in oriental fruit fly, Bactrocera dorsalis (Hendel)

Antimicrobial peptide gene BdPho responds to peptidoglycan infection and mating stimulation in oriental fruit fly, Bactrocera dorsalis (Hendel)

The immune response of Locusta migratoria manilensis at different times of infection with Paranosema locustae

Potential targets for controlling Bactrocera dorsalis using cuticle‐ and hormone‐related genes revealed by a developmental transcriptome analysis

Contact Info

Product

Resources

About