Antimicrobial peptide Cathelicidin-BF prevents intestinal barrier dysfunction in a mouse model of endotoxemia

Song, Dong‐Ping; Zong, Xin; Zhang, Haiwen; Wang, Tenghao; Yi, Hongbo; Luan, Chao; Wang, Yizhen

doi:10.1016/j.intimp.2015.01.017

Cited by 54 publications

(36 citation statements)

References 35 publications

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“…In animal model of sepsis, cathelicidin prevented intestinal barrier dysfunction (18). LL-37 is the only cathelicidin-derived anti-microbial peptide found in humans.…”

Section: Resultsmentioning

confidence: 94%

Successful Therapy of Clostridium Difficile Infection with Fecal Microbiota Transplantation

Konturek¹,

Dieterich²,

Neurath³

et al. 2017

Gastroenterology

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“…In animal model of sepsis, cathelicidin prevented intestinal barrier dysfunction (18). LL-37 is the only cathelicidin-derived anti-microbial peptide found in humans.…”

Section: Resultsmentioning

confidence: 94%

Successful Therapy of Clostridium Difficile Infection with Fecal Microbiota Transplantation

Konturek¹,

Dieterich²,

Neurath³

et al. 2017

Gastroenterology

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“…In addition, venom has been shown to have pro- and anti-inflammatory properties and has been shown to regulate the expression of inflammatory mediators such as IL-1β, IL-6, IL-10, IL-22, TNF-α, PGD2, PGE2, and TXA2 [18,19,21,41,42,43,44]. In this study, we constructed and screened an H. cyanocinctus venom gland T7 phage display library and discovered H-TL1, a natural peptide with antagonizing effects against TNF-α.…”

Section: Discussionmentioning

confidence: 99%

“…Cathelicidin-BF (C-BF), a new cathelicidin peptide purified from Bungarus fasciatus snake venom, has shown both antimicrobial and anti-inflammatory properties. Moreover, C-BF decreases the expression and secretion of TNF-α [18,19]. Venom from sea snakes is more streamlined and stable than that from land snakes [20], and Hydrophis cyanocinctus , a primary sea snake species in China, has been used to treat rheumatism for centuries.…”

Section: Introductionmentioning

confidence: 99%

Hydrostatin-TL1, an Anti-Inflammatory Active Peptide from the Venom Gland of Hydrophis cyanocinctus in the South China Sea

Wang

Huang

Liu

et al. 2016

IJMS

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Tumor necrosis factor (TNF)-α is a pleiotropic cytokine with intense pro-inflammatory and immunomodulatory properties, and anti-TNF-α biologics are effective therapies for various inflammatory diseases such as inflammatory bowel disease (IBD) and sepsis. Snake venom, as a traditional Chinese medicine, has been used in the treatment of inflammatory diseases in China for centuries. In this research, we constructed a venom gland T7 phage display library of the sea snake Hydrophis cyanocinctus to screen bioactive compounds that antagonize TNF-α and identified a novel nine-amino-acid peptide, termed hydrostatin-TL1 (H-TL1). In enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR) analyses, H-TL1 inhibited the interaction between TNF-α and TNF receptor 1 (TNFR1). Further, H-TL1 attenuated the cytotoxicity of TNF-α in L929 cells as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. H-TL1 also decreased the mRNA expression of TNF-α/TNFR1 downstream targets and suppressed the phosphorylation of well-characterized proteins of downstream signal transduction pathways in HEK-293 cells. In vivo data demonstrated that H-TL1 protects animals against dextran sodium sulfate (DSS)-induced acute colitis and lipopolysaccharide (LPS)-induced acute shock. Given its significant anti-inflammatory activity in vitro and in vivo, H-TL1 is a potential peptide for the development of new agents to treat TNF-α-associated inflammatory diseases.

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“…Interestingly, in a study using a mouse model of endotoxemia as induced by an intraperitoneal injection of lipopolysaccharide, Bungarus fasciatus cathelicidin precluded the damage and the cell death that were provoked by LPS to the intestinal epithelium. Moreover, cathelicidin BF inhibited the expression and secretion of tumor necrosis factor a (TNF-a) in mouse macrophages (Song et al 2015).…”

Section: Vipericidins and Snake Venom Cathelicidin-related Peptidesmentioning

confidence: 99%

Vipericidins, Snake Venom Cathelicidin-Related Peptides, in the Milieu of Reptilian Antimicrobial Polypeptides

Rádis‐Baptista¹

2015

Snake Venoms

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After the "antibiotic age" we are experiencing a "post-antibiotic era", in which our current antimicrobial arsenal is expiring. In addition, drug-resistant infectious diseases have emerged and reemerged. Antimicrobial peptides (AMPs) arose as an alternative to classical antibiotic drugs. AMPs are selective membrane-active compounds with a wide spectrum of action against bacteria, fungi, parasites, and viruses. Due to their properties, AMPs are also effective as anticancer peptides and some AMPs can connect the innate and acquired immunity. To date, thousands of sequences have been described from a wide range of phyla. In reptilians, the predominant classes of AMPs that have been found until now encompass b-defensins and the cathelicidins. Cathelicidin-related antimicrobial peptides (CRAMPs) have been characterized from Asian elapids and South American pit vipers. Vipericidins from rattlesnakes and jararacas and elapid CRAMPs from cobra and kraits consist of a signal peptide, a conserved cathelin domain, and variable carboxyl-terminal sequences of linear a-helical peptides, from where the antimicrobials are released. Full and short synthetic versions of vipericidins and elapid CRAMPs have been prepared and possess a distinct efficacy toward microbial and transformed malignant cells. Although not belonging to the class of the AMPs, venom polypeptides with biocide activity comprise enzymatic toxins (e.g., PLA2) and nonenzymatic waprins. Altogether, animal venom constitutes a rich source for the disclosure of AMPs with diverse sequences and multiple functions. Given the current knowledge, venomderived AMPs offer a multitude of possibilities for understanding the evolution of this immune-effector molecule and for generating engineered peptides by de novo design.

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Antimicrobial peptide Cathelicidin-BF prevents intestinal barrier dysfunction in a mouse model of endotoxemia

Cited by 54 publications

References 35 publications

Successful Therapy of Clostridium Difficile Infection with Fecal Microbiota Transplantation

Successful Therapy of Clostridium Difficile Infection with Fecal Microbiota Transplantation

Hydrostatin-TL1, an Anti-Inflammatory Active Peptide from the Venom Gland of Hydrophis cyanocinctus in the South China Sea

Vipericidins, Snake Venom Cathelicidin-Related Peptides, in the Milieu of Reptilian Antimicrobial Polypeptides

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