Antimicrobial peptide-based array for Escherichia coli and Salmonella screening

Kulagina, N. V.; Shaffer, Kara M.; Anderson, George P.; Ligler, Frances S.; Taitt, Chris R.

doi:10.1016/j.aca.2006.05.082

Cited by 99 publications

(92 citation statements)

References 27 publications

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“…Current dogma dictates that the antimicrobial activity of these peptides is mediated through interaction of AMPs with target cell membranes and subsequent membrane disruption, although other mechanisms have been postulated [12][13][14]. The current study expands on previous work developing AMP-based detection assays for Escherichia coli and Salmonella typhimurium [15,16]. As a first step in development of a multiplexed system for biothreat detection, direct assays for two intracellular pathogens (Coxiella burnetti, Brucella melitensis) and two enveloped viruses (Venezuelan equine encephalitis [VEE], vaccinia virus) were demonstrated using fluorescently labeled targets.…”

Section: Introductionsupporting

confidence: 61%

Antimicrobial peptides as new recognition molecules for screening challenging species

Kulagina

Shaffer

Ligler

et al. 2007

Sensors and Actuators B: Chemical

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The goal of this study was to evaluate binding of four targets of biodefense interest to immobilized antimicrobial peptides (AMPs) in biosensor assays. Polymyxins B and E, melittin, cecropins A, B, and P, parasin, bactenecin and magainin-1, as well as control antibodies, were used as capture molecules for detection of Cy3-labeled Venezuelan equine encephalitis virus (VEE), vaccinia virus, C. burnetti and B. melitensis. Although VEE, vaccinia virus and C. burnetti did not show any binding activity to their corresponding capture antibodies, B. melitensis bound to immobilized antiBrucella monoclonal antibodies. The majority of the immobilized AMPs included in this study bound labeled VEE, vaccinia virus and C. burnetti in a concentration-dependent manner, and B. melitensis bound to polymyxin B, polymyxin E, and bactenecin. No binding was observed on immobilized magainin-1. In contrast to all bacterial targets tested to date, VEE and vaccinia virus demonstrated similar patterns of binding to all peptides. While the direct assay is generally replaced by a sandwich assay for analysis of real-world samples, direct binding experiments are commonly used to characterize specificity and sensitivity of binding molecules. In this case, they clearly demonstrate the capability of AMPs as recognition molecules for four biothreat agents.

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Section: Introductionsupporting

confidence: 61%

Antimicrobial peptides as new recognition molecules for screening challenging species

Kulagina

Shaffer

Ligler

et al. 2007

Sensors and Actuators B: Chemical

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show abstract

“…Another recent study has demonstrated the ability of naturally occurring antimicrobial peptides (AMPs) to serve as robust biorecogniton moieties in electronic biosensing platforms 30 . Unlike antibodies, AMPs are significantly more stable and exhibit broadband detection for a range of pathogenic bacteria 31,32 .…”

mentioning

confidence: 99%

Graphene-based wireless bacteria detection on tooth enamel

et al. 2012

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Direct interfacing of nanosensors onto biomaterials could impact health quality monitoring and adaptive threat detection. Graphene is capable of highly sensitive analyte detection due to its nanoscale nature. Here we show that graphene can be printed onto water-soluble silk. This in turn permits intimate biotransfer of graphene nanosensors onto biomaterials, including tooth enamel. The result is a fully biointerfaced sensing platform, which can be tuned to detect target analytes. For example, via self-assembly of antimicrobial peptides onto graphene, we show bioselective detection of bacteria at single-cell levels. Incorporation of a resonant coil eliminates the need for onboard power and external connections. Combining these elements yields two-tiered interfacing of peptide-graphene nanosensors with biomaterials. In particular, we demonstrate integration onto a tooth for remote monitoring of respiration and bacteria detection in saliva. overall, this strategy of interfacing graphene nanosensors with biomaterials represents a versatile approach for ubiquitous detection of biochemical targets.

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“…Antimicrobial peptides are naturally occurring molecules that serve as part of the innate immune system of many organisms and work by disrupting cell membranes. Kulagina and coworkers immobilized magainin I and other antimicrobial peptides onto glass slides and were able to measure Cy5-labeled Escherichia coli O157:H7 and Salmonella typhimurium, 42,43 with limits of detection in the same range as analogous antibody-based assays. Other microbial species (including inactivated preparations of Brucella melitensis, Coxiella burnetti, and two enveloped viruses) 44 have also been detected on the NRL Array Biosensor using antimicrobial peptide-based assays.…”

Section: Detection Based On Non-antibody Moleculesmentioning

confidence: 99%

“…A significant enhancement in stability was demonstrated with antimicrobial peptides and clear differences in binding patterns of the bacterial species were demonstrated. 43,44 Both carbohydrates and antimicrobial peptides bind semiselectively and are capable of interacting with multiple targets. A multi-peptide or multi-carbohydrate array could therefore complement the more selective antibody capture immunoassays in a multi-capture array format.…”

Section: Detection Based On Non-antibody Moleculesmentioning

confidence: 99%