Antimicrobial mechanism of alkyl gallates against Escherichia coli and Staphylococcus aureus and its combined effect with electrospun nanofibers on Chinese Taihu icefish preservation

Shi, Yixuan; Zhang, Runrun; Zhu, Chen-min; Xu, Mingfeng; Gu, Qing; Ettelaie, Rammile; Lin, Shan; Wang, Yifan; Leng, Xinyi

doi:10.1016/j.foodchem.2020.128949

Cited by 46 publications

(50 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ROS are highly active chemical substances, and their main types are free radicals, including singlet oxygen ( 1 O 2 ), superoxide anion radicals (·O 2 − ), and highly active hydroxyl radicals (·OH). [ 10 ] Because of their high oxidation activity, ROS are found to work as antimicrobial substances by destroying organic components, including proteins and DNA, of biological cells. [ 11 ] However, to date, there is rare reports on the application of single atoms in the production of ROS and antimicrobial activity of single atoms.…”

Section: Introductionmentioning

confidence: 99%

Amidoxime Group‐Anchored Single Cobalt Atoms for Anti‐Biofouling during Uranium Extraction from Seawater

et al. 2022

View full text Add to dashboard Cite

Marine biofouling is one of the most significant challenges hindering practical uranium extraction from seawater. Single atoms have been widely used in catalytic applications because of their remarkable redox property, implying that the single atom is highly capable of catalyzing the generation of reactive oxygen species (ROS) and acts as an anti-biofouling substance for controlling biofouling. In this study, the Co single atom loaded polyacrylamidoxime (PAO) material, PAO-Co, is fabricated based on the binding ability of the amidoxime group to uranyl and cobalt ions. Nitrogen and oxygen atoms from the amidoxime group stabilize the Co single atom. The fabricated PAO-Co exhibits a broad range of antimicrobial activity against diverse marine microorganisms by producing ROS, with an inhibition rate up to 93.4%. The present study is the first to apply the single atom for controlling biofouling. The adsorbent achieves an ultrahigh uranium adsorption capacity of 9.7 mg g −1 in biofouling-containing natural seawater, which decreased only by 11% compared with that in biofouling-removed natural seawater. These findings indicate that applying single atoms would be a promising strategy for designing biofouling-resistant adsorbents for uranium extraction from seawater.

show abstract

Section: Introductionmentioning

confidence: 99%

Amidoxime Group‐Anchored Single Cobalt Atoms for Anti‐Biofouling during Uranium Extraction from Seawater

et al. 2022

View full text Add to dashboard Cite

show abstract

“…It is also in accordance with the findings from Kubo et al . and us . (2) As for the OG-mediated PDI, OG alone or UV-A light irradiation was not solely responsible for the remarkable microbial reduction, which further corroborates the occurrence of interaction between OG and UV-A light.…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, it also indicated that ROS production induced by OG itself could be essential for OG-induced killing in the dark, which is in agreement with our previous report that the internalized OG may interfere with the activity of the electron transport chain (ETC) on the cytoplasmic membrane of E. coli, promoting the generation of toxic ROS and consequently leading to bacterial death . Furthermore, the ROS was visualized by CLSM (Figure B).…”

Section: Resultsmentioning

confidence: 99%

“…In our most recent study, a series of alkyl gallates were prepared using lipase-catalyzed reactions. Among them, octyl gallate (OG) exhibited excellent antibacterial activity against some related foodborne pathogens . Surprisingly, the potential of OG with low concentration as a novel PS for PDI has been found in this work, which endows OG-mediated PDI with intriguing bactericidal efficacy to achieve rapid eradication of pathogens and biofilms in a relatively short time due to both photodynamic and intrinsic antibacterial properties of OG itself.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

New Horizons in Microbiological Food Safety: Ultraefficient Photodynamic Inactivation Based on a Gallic Acid Derivative and UV-A Light and Its Application with Electrospun Cyclodextrin Nanofibers

Shi

Zhu

et al. 2021

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

An excellent bactericidal effect of octyl gallate (OG)-mediated photodynamic inactivation (PDI) against foodborne pathogens (Escherichia coli and Staphylococcus aureus) was evaluated in relation to the mode of action. UV-A irradiation (wavelength, 365 nm; irradiance, 8.254 ± 0.18 mW/cm 2 ) of the bacterial suspension containing 0.15 mM OG could lead to a >5-log reduction of viable cell counts within 30 min for E. coli and only 5 min for S. aureus. Reactive oxygen species (ROS) formation was considered the main reason for the bactericidal effect of OG + UV-A light treatment because toxic ROS induced by OG-mediated PDI could attack the cellular wall, proteins, and DNA of microbes. Moreover, the bactericidal effect, as well as the yields of ROS, depended on OG concentrations, irradiation time, and laser output power. Furthermore, we prepared an edible photodynamic antimicrobial membrane comprising electrospun cyclodextrin nanofibers (NFs) by embedding OG. The resultant OG/HPβCD NFs (273.6 μg/ mL) under UV-A irradiation for 30 min (14.58 J/cm) could cause a great reduction (>5-log) of viable bacterial counts of E. coli. The in situ photodynamic antibacterial activity of OG/HPβCD NF-based packaging was evaluated during the Chinese giant salamander storage. Overall, this research highlights the dual functionalities (antibacterial and photodynamic properties) of OG as both an antibacterial agent and photosensitizer and the effectiveness of electrospun NFs containing OG as an active antibacterial packaging material for food preservation upon UV light illumination.

show abstract

“…Bacterial survival mechanisms regulate cellular metabolites and proteins through gene expression in response to exposure to different pH levels, temperature, osmotic pressure, and toxic chemicals, thereby maximizing the chance of survival [ 13 , 14 ]. For example, S. aureus overexpresses the csp family gene to enhance its cold stress response at low temperatures [ 15 ]; S. aureus undergoes exhibits of amino acids, citric acid, and β-aminoisobutyric acid after cold stress, allowing the bacteria to adapt to long-term low-temperature environments [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptomic Analysis of Staphylococcus aureus Reveals the Genes Involved in Survival at Low Temperature

Suo

Guan

Dong

et al. 2022

Foods

View full text Add to dashboard Cite

In food processing, the temperature is usually reduced to limit bacterial reproduction and maintain food safety. However, Staphylococcus aureus can adapt to low temperatures by controlling gene expression and protein activity, although its survival strategies normally vary between different strains. The present study investigated the molecular mechanisms of S. aureus with different survival strategies in response to low temperatures (4 °C). The survival curve showed that strain BA-26 was inactivated by 6.0 logCFU/mL after 4 weeks of low-temperature treatment, while strain BB-11 only decreased by 1.8 logCFU/mL. Intracellular nucleic acid leakage, transmission electron microscopy, and confocal laser scanning microscopy analyses revealed better cell membrane integrity of strain BB-11 than that of strain BA-26 after low-temperature treatment. Regarding oxidative stress, the superoxide dismutase activity and the reduced glutathione content in BB-11 were higher than those in BA-26; thus, BB-11 contained less malondialdehyde than BA-26. RNA-seq showed a significantly upregulated expression of the fatty acid biosynthesis in membrane gene (fabG) in BB-11 compared with BA-26 because of the damaged cell membrane. Then, catalase (katA), reduced glutathione (grxC), and peroxidase (ahpC) were found to be significantly upregulated in BB-11, leading to an increase in the oxidative stress response, but BA-26-related genes were downregulated. NADH dehydrogenase (nadE) and α-glucosidase (malA) were upregulated in the cold-tolerant strain BB-11 but were downregulated in the cold-sensitive strain BA-26, suggesting that energy metabolism might play a role in S. aureus under low-temperature stress. Furthermore, defense mechanisms, such as those involving asp23, greA, and yafY, played a pivotal role in the response of BB-11 to stress. The study provided a new perspective for understanding the survival mechanism of S. aureus at low temperatures.

show abstract

Antimicrobial mechanism of alkyl gallates against Escherichia coli and Staphylococcus aureus and its combined effect with electrospun nanofibers on Chinese Taihu icefish preservation

Cited by 46 publications

References 42 publications

Amidoxime Group‐Anchored Single Cobalt Atoms for Anti‐Biofouling during Uranium Extraction from Seawater

Amidoxime Group‐Anchored Single Cobalt Atoms for Anti‐Biofouling during Uranium Extraction from Seawater

New Horizons in Microbiological Food Safety: Ultraefficient Photodynamic Inactivation Based on a Gallic Acid Derivative and UV-A Light and Its Application with Electrospun Cyclodextrin Nanofibers

Comparative Transcriptomic Analysis of Staphylococcus aureus Reveals the Genes Involved in Survival at Low Temperature

Contact Info

Product

Resources

About