Antimicrobial magnetic poly(GMA) microparticles: synthesis, characterization and lysozyme immobilization

Abstract

Micron-sized magnetic particles currently find a wide range of applications in many areas including biotechnology, biochemistry, colloid sciences and medicine. In this study, magnetic poly(glycidyl methacrylate) microparticles were synthesized by providing a polymerization around Fe(II)-Ni(II) magnetic double salt. Adsorption of lysozyme protein from aqueous systems was studied with these particles. Adsorption studies were performed with changing pH values, variable amount of adsorbent, different interaction times and lysozyme amounts. The adsorption capacity of the particles was investigated, and a value of about 95.6 mg lysozyme/g microparticle was obtained. The enzyme activity of the immobilized lysozyme was examined and found to be more stable and reusable compared to the free enzyme. The immobilized enzyme still showed 80% activity after five runs and managed to maintain 78% of its initial activity at the end of 60 days. Besides, in the antimicrobial analysis study for six different microorganisms, the minimum inhibitory concentration value of lysozyme immobilized particles was calculated as 125 μg/mL like free lysozyme. Finally, the adsorption interaction was found to be compatible with the Langmuir isotherm model. Accordingly, it can be said that magnetic poly(GMA) microparticles are suitable materials for lysozyme immobilization and immobilized lysozyme can be used in biotechnological studies.

Antibiotic Removal from the Aquatic Environment with Activated Carbon Produced from Pumpkin Seeds

“…The porous nature of construction is considered a critical advantage for adsorption [30][31][32][33][34], since the target molecule's attachment to and its retention in When the adsorbent's SEM analysis is examined, it is particularly striking that it has a porous structure (Figure 2). The porous nature of construction is considered a critical advantage for adsorption [30][31][32][33][34], since the target molecule's attachment to and its retention in the pore during interaction can be interpreted as a characteristic that facilitates adsorption. In addition, the fact that the adsorbed molecule is desorbed from the surface by various external factors complicates interactions within the pore.…”

Adsorptive removal of cationic dye from aqueous solutions using Bardakçı clay

“…The adsorption was found to be suitable for the Langmuir model, as shown in Table 2. In other words, it demonstrates that the adsorption is monolayer and homogeneous (İhsan Alacabey et al, 2020;Erol, Tatar, Veyisoğlu, & Tokatlı, 2021). It was determined that the RL separation factors of 0.0162 ≤ RL ≤ 0.2527 for the CV dyestuff on the BK adsorbent were between 0 and 1, which indicated favourable adsorption (Table 3).…”

Endosulfan Elimination Using Amine-Modified Magnetic Diatomite as an Adsorbent

“…The adsorption time of endosulfan adsorption on both m-DE-APTES and DE-APTES was investigated via the batch system (Erol et al, 2020;Erol and Uzun, 2017;Erol, 2017a;Erol, 2017b;Kireç et al, 2021;Erol et al, 2021;Erol, 2017c;Tosun Satir and Erol, 2021) at 25 °C. Throughout the experiments, four different endosulfan concentrations (100.0-750.0 mg L −1 ) were used at various times (10-60 min) (Figure 6). As shown in Figure 5, the adsorption capacity of m-DE-APTES for endosulfan increased within 45 min and then reached equilibrium at the 60th minute.…”