Antimicrobial lipid capped copper sulfide nanoparticles display enhanced bactericidal effect against Carbapenem-Resistant Acinetobacter baumannii

Singaravelu, Dharshini Karnan; Subramaniyan, Siva Bala; Tharunya, M P; Veerappan, Anbazhagan

doi:10.1016/j.matlet.2021.130985

Cited by 9 publications

(9 citation statements)

References 11 publications

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Section: Resultsmentioning

confidence: 99%

“…Cationic copper sulfide (cCuS) nanoparticles were prepared using the capping agent [2-(( N -(2-hydroxyethyl)palmitamido)methyl)-1-methylpyridin-1-ium iodide] (cN16E). 22 The surface charge of the NPs was quantitatively described by zeta potential. A positive zeta potential value of +10.0 mV suggested that the effective electric charge of the NP surface was positive, which may aid the NPs in recognizing the negative surface charge of bacteria.…”

Section: Resultsmentioning

confidence: 99%

“…The 2-(( N -(2-hydroxyethyl)palmitamido)methyl)-1-methylpyridin-1-ium iodide-capped copper sulfide (cCuS) nanoparticles were prepared as described previously . The binding between jacalin and cCuS was monitored in a JASCO-FP8200 spectrometer.…”

Section: Methodsmentioning

confidence: 99%

“…The 2-(( N -(2-hydroxyethyl)palmitamido)methyl)-1-methylpyridin-1-ium iodide-capped copper sulfide (cCuS) nanoparticles were prepared as described previously. 22 The binding between jacalin and cCuS was monitored in a JASCO-FP8200 spectrometer. Briefly, 5 μM jacalin (3 mL) was titrated by adding small aliquots of 1 mM cCuS, and the change in jacalin fluorescence was monitored from 300 to 400 nm in a quartz cell upon excitation at 280 nm.…”

Section: Methodsmentioning

confidence: 99%

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Lectin-Fortified Cationic Copper Sulfide Nanoparticles Gain Dual Targeting Capabilities to Treat Carbapenem-Resistant Acinetobacter baumannii Infection

et al. 2022

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Targeted drug delivery maximizes the chance to combat infection caused by drug-resistant pathogens. Herein, lectin-fortified cationic copper sulfide (cCuS) nanoparticles were suggested for targeted adhesion to bacterial membranes and to enforce bacterial death. Jacalin, a lectin from jackfruit seed, was conjugated to fluorescein isothiocyanate (FITC), and its ability to recognize bacterial cell surface glycans was demonstrated. Jacalin formed a noncovalent complex with cCuS, which was investigated by fluorescence quenching measurements. The data revealed that jacalin–cCuS (JcCuS) had a good affinity with an association constant K a of 2.27 (± 0.28) × 104 M–1. The resultant JcCuS complex displayed excellent anti-infective activity against carbapenem-resistant Acinetobacter baumannii (CRAB). The minimum inhibitory concentration (MIC) of cCuS was 62.5 μM, which was 2-fold lower than that of the broad-spectrum antibiotic ciprofloxacin. Interestingly, the MIC of JcCuS was reduced to 15.63 μM, which was attributed to jacalin fortification. The mechanistic study unveiled that JcCuS affected the membrane integrity, depolarized the inner membrane, and produced excess reactive oxygen species to combat CRAB at a lower concentration compared to cCuS. A. baumannii formed a biofilm more readily, which played a critical role in pathogenesis and resistance in clinical settings. JcCuS (3.91 μM) displayed stronger antibiofilm activity without affecting the metabolic viability of CRAB. Microscopy analyses confirmed the inhibition of biofilm formation and disruption of the mature biofilm upon treatment with JcCuS. Furthermore, JcCuS hindered pellicle formation and inhibited the biofilm-associated virulence factor of CRAB such as exopolysaccharide, cell surface hydrophobicity, swarming, and twitching mobility. The anti-infective potential of JcCuS was demonstrated by rescuing CRAB-infected zebrafish. The reduction in pathogen proliferation in muscle tissues was observed in the treated group, and the fish recovered from the infection and was restored to normal life within 12 h. The findings illustrate that lectin fortification offers a unique advantage in enhancing the therapeutic potential of antimicrobials against human pathogens of critical priority worldwide.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Lectin-Fortified Cationic Copper Sulfide Nanoparticles Gain Dual Targeting Capabilities to Treat Carbapenem-Resistant Acinetobacter baumannii Infection

et al. 2022

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“…On the other hand, the antimicrobial effect of CuS nanoparticles has been scarcely reported, even though these NPs are much less toxic for microorganisms than copper oxide or Cu 0 nanoparticles. ROS production mediated by the Cu ions released from the nanostructure is the main cause of the cellular effects of CuS NPs ( Figure 1 ) [ 91 , 92 ]. However, the antimicrobial effect of these nanoparticles could also be due to their nanometric size, composition, and structure [ 93 , 94 , 95 ].…”

Section: Copper Toxicity Mechanismsmentioning

confidence: 99%

Toxicity Mechanisms of Copper Nanoparticles and Copper Surfaces on Bacterial Cells and Viruses

Ramos-Zúñiga

Bruna

Pérez-Donoso

2023

IJMS

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Copper is a metal historically used to prevent infections. One of the most relevant challenges in modern society are infectious disease outbreaks, where copper-based technologies can play a significant role. Currently, copper nanoparticles and surfaces are the most common antimicrobial copper-based technologies. Despite the widespread use of copper on nanoparticles and surfaces, the toxicity mechanism(s) explaining their unique antimicrobial properties are not entirely known. In general, toxicity effects described in bacteria and fungi involve the rupture of membranes, accumulation of ions inside the cell, protein inactivation, and DNA damage. A few studies have associated Cu-toxicity with ROS production and genetic material degradation in viruses. Therefore, understanding the mechanisms of the toxicity of copper nanoparticles and surfaces will contribute to developing and implementing efficient antimicrobial technologies to combat old and new infectious agents that can lead to disease outbreaks such as COVID-19. This review summarizes the current knowledge regarding the microbial toxicity of copper nanoparticles and surfaces and the gaps in this knowledge. In addition, we discuss potential applications derived from discovering new elements of copper toxicity, such as using different molecules or modifications to potentiate toxicity or antimicrobial specificity.

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Stirring effect of CuS deposition over cotton thread for photocatalysis and electrical application

Khushboo,

Sharma,

Singh

et al. 2023

Chemical Physics Impact

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Antimicrobial lipid capped copper sulfide nanoparticles display enhanced bactericidal effect against Carbapenem-Resistant Acinetobacter baumannii

Cited by 9 publications

References 11 publications

Lectin-Fortified Cationic Copper Sulfide Nanoparticles Gain Dual Targeting Capabilities to Treat Carbapenem-Resistant Acinetobacter baumannii Infection

Lectin-Fortified Cationic Copper Sulfide Nanoparticles Gain Dual Targeting Capabilities to Treat Carbapenem-Resistant Acinetobacter baumannii Infection

Toxicity Mechanisms of Copper Nanoparticles and Copper Surfaces on Bacterial Cells and Viruses

Stirring effect of CuS deposition over cotton thread for photocatalysis and electrical application

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