Antimicrobial flavonoids isolated from Indian medicinal plant<i>Scutellaria oblonga</i>inhibit biofilms formed by common food pathogens

Rajendran, N.; Subramaniam, Shankar; Christena, Lowrence Rene; Muthuraman, Meenakshi Sundaram; Nagarajan, Saisubramanian; Brindha, P.; Sivasubramanian, Aravind

doi:10.1080/14786419.2015.1104673

Cited by 33 publications

(24 citation statements)

References 15 publications

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“…The strongest antibiofilm activities were evidenced for Quer-3-O-Glc (31%) and GA (28%), both at 50 µg/mL concentration, while QA exhibited only a stimulatory effect on biofilm formation regardless of the concentration. The obtained results were in accordance with published data [21][22][23][24]. The interference of the extracts and pure compounds with three major interconnected QS systems of P. aeruginosa: las, rhl and pqs, was next addressed.…”

Section: Identification Of Qs Signalling Pathways Affected By Extractsupporting

confidence: 86%

Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts

et al. 2020

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Many traditional remedies represent potential candidates for integration with modern medical practice, but credible data on their activities are often scarce. For the first time, the anti-virulence potential and the safety for human use of the ethanol extracts of two medicinal plants, Persicaria maculosa (PEM) and Bistorta officinalis (BIO), have been addressed. Ethanol extracts of both plants exhibited anti-virulence activity against the medically important opportunistic pathogen Pseudomonas aeruginosa. At the subinhibitory concentration of 50 µg/mL, the extracts demonstrated a maximal inhibitory effect (approx. 50%) against biofilm formation, the highest reduction of pyocyanin production (47% for PEM and 59% for BIO) and completely halted the swarming motility of P. aeruginosa. Both extracts demonstrated better anti-quorum sensing and antibiofilm activities, and a better ability to interfere with LasR receptor, than the tested dominant extracts' constituents. The bioactive concentrations of the extracts were not toxic in the zebrafish model system. This study represents an initial step towards the integration of P. maculosa and B. officinalis for use in the treatment of Pseudomonas infections.

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Section: Identification Of Qs Signalling Pathways Affected By Extractsupporting

confidence: 86%

Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts

et al. 2020

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“…Since many plant bacterial pathogens use type III secretion systems to cause disease and sense flavonoids 30 , it is possible that some of these compounds may have evolved as a consequence host-pathogen interactions. Moreover, flavonoids have also been reported to target other anti-infective properties such as expression of virulence genes 31,32 , toxin activity 33-35 , quorum sensing 36-39 and biofilm formation 40,41 in bacterial pathogens as well as attenuate destructive host inflammation 42-44 . Given the broad pharmacological activities of flavonoids, these plant metabolites may affect microbial virulence mechanisms, including type III protein secretion substrates shown here, in addition to host pathways for their anti-infective properties in vivo .…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Flavonoids from Medicinal Plants Covalently Inactivate Type III Protein Secretion Substrates

et al. 2016

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Traditional Chinese Medicines (TCMs) have been historically used to treat bacterial infections. However, the molecules responsible for these anti-infective properties and their potential mechanisms of action have remained elusive. Using a high-throughput assay for type III protein secretion in Salmonella enterica serovar Typhimurium, we discovered that several TCMs can attenuate this key virulence pathway without affecting bacterial growth. Amongst the active TCMs, we discovered that baicalein, a specific flavonoid from Scutellaria baicalensis, targets S. Typhimurium pathogenicity island-1 (SPI-1) type III secretion system (T3SS) effectors and translocases to inhibit bacterial invasion of epithelial cells. Structurally related flavonoids present in other TCMs, such as quercetin also inactivated the SPI-1 T3SS and attenuated S. Typhimurium invasion. Our results demonstrate that specific plant metabolites from TCMs can directly interfere with key bacterial virulence pathways and reveals a previously unappreciated mechanism of action for anti-infective medicinal plants.

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“…In another study, among the nine flavonoids (from the leaves of Scutellaria oblonga ), quercitin‐3‐glucoside ( 65 ) could successfully kill S . aureus and reduction in biofilms (90–95%) was observed (Rajendran et al, ). Other reports of the antibacterial activity of flavonols are summarized in Table .…”

Section: Antibacterial Activity Of Flavonoidsmentioning

confidence: 97%

“…Evaluation of the MOA of flavonoid compounds from Piper species [ 174 and 2′,4′,4‐trihydroxy‐3,6′‐dimethoxychalchone ( 121 )] against Vibrio harveyi exhibited a strong dose‐dependent inhibition of biofilm formation without effect on bacterial growth up to 500 μM (Martín‐Rodríguez et al, ). In the study of three flavonoids [techtochrysin ( 30 ), negletein ( 31 ), and quercitin‐3‐glucoside ( 65 )] against foodborne pathogens, 90–95% reduction in biofilms was observed (Rajendran et al, ). Synthesized tricyclic flavonoid ( 153 ) at low concentration caused not only the inhibition of bacterial growth (MIC: 0.24 μg/ml) but also killing bacterial cells via cell membrane integrity and cell agglutination (Babii et al, ).…”

Section: Mechanism Of Antibacterial Activitymentioning

confidence: 99%

Antibacterial activity of flavonoids and their structure–activity relationship: An update review

Farhadi

Khameneh

Iranshahi

et al. 2018

Phytotherapy Research

460

305

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Based on World Health Organization reports, resistance of bacteria to well-known antibiotics is a major global health challenge now and in the future. Different strategies have been proposed to tackle this problem including inhibition of multidrug resistance pumps and biofilm formation in bacteria and development of new antibiotics with novel mechanism of action. Flavonoids are a large class of natural compounds, have been extensively studied for their antibacterial activity, and more than 150 articles have been published on this topic since 2005. Over the past decade, some promising results were obtained with the antibacterial activity of flavonoids. In some cases, flavonoids (especially chalcones) showed up to sixfold stronger antibacterial activities than standard drugs in the market. Some synthetic derivatives of flavonoids also exhibited remarkable antibacterial activities with 20-to 80-fold more potent activity than the standard drug against multidrug-resistant Gram-negative and Gram-positive bacteria (including Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus). This review summarizes the ever changing information on antibacterial activity of flavonoids since 2005, with a special focus on the structure-activity relationship and mechanisms of actions of this broad class of natural compounds.

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Antimicrobial flavonoids isolated from Indian medicinal plantScutellaria oblongainhibit biofilms formed by common food pathogens

Cited by 33 publications

References 15 publications

Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts

Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts

Antibacterial Flavonoids from Medicinal Plants Covalently Inactivate Type III Protein Secretion Substrates

Antibacterial activity of flavonoids and their structure–activity relationship: An update review

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