Antimicrobial ent-pimarane diterpenes from Viguiera arenaria against Gram-positive bacteria

Abstract

The dichloromethane crude extract from the roots of Viguiera arenaria (VaDRE) has been employed in an antimicrobial screening against several bacteria responsible for human pathologies. The main diterpenes isolated from this extract, as well as two semi-synthetic pimarane derivatives, were also investigated for the pathogens that were significantly inhibited by the extract (MIC values lower than 100 microg mL(-1)). The VaDRE extract was significantly active only against Gram-positive microorganisms. The compounds ent-pimara-8(14),15-dien-19-oic acid (PA); PA sodium salt; ent-8(14),15-pimaradien-3beta-ol; ent-15-pimarene-8 beta,19-diol; and ent-8(14),15-pimaradien-3beta-acetoxy displayed the highest antibacterial activities (MIC values lower than 10 microg mL(-1) for most pathogens). In conclusion, our results suggest that pimaranes are an important class of natural products for further investigations in the search of new antibacterial agents.

Bactericidal Kinetics and Antibiofilm Efficacy of Pimarane-Type Diterpenes from Viguiera arenaria Against Cariogenic Bacteria

“…Figure 1 shows the bactericidal kinetics of different concentrations of compound 1 against cariogenic bacteria in the planktonic mode, for different periods of incubation. In a previous paper, 13 established the MBC concentrations evaluated in the present study. Here, we also assayed two and three times the MBC values for each bacteria.…”

“…11 Among the various classes of metabolites, diterpenes are recognized as a class displaying a wide spectrum of biological activities including their significant antibacterial activity. [12][13][14][15][16][17] The species V. arenaria contains a class of pimarane type-diterpenes that have been proven to exhibit potential action against cariogenic bacteria, as reported in previous studies by our research group. Indeed, 12 have found Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values lower than 10 µg/mL for such diterpenes.…”

Antibiofilm activity of natural substances derived from plants

“…Its frequent and prolonged use presents many collateral effects, such as changes in the taste of food and a burning feeling on the tip of the tongue (Greenberg et al, 2008;More et al, 2008;Porto et al, 2009).…”

Diterpenes of the pimarane type isolated from Viguiera arenaria: Promising in vitro biological potential as therapeutic agents for endodontics

“…Diterpenes also display significant antimicrobial activity (Tatsimo et al, 2005;Kuzma et al, 2007;Ndi et al, 2007;Fukumoto et al, 2008;Porto et al, 2009a;Porto et al, 2009b;Carvalho et al, 2011;Andrade et al, 2011;Moreira et al, 2016;Moreti et al, 2017;Veneziani et al, 2017). Ambrosio et al (2004) isolated and identified diterpenes of the pimarane type from V. arenaria baker root extracts.…”