Antimicrobial Efficacy of Selected Ayurveda Formula against Laboratory Specimen of Staphylococcus aureus

Abstract

Selected formula consisted of three ingredients including dried leaves of Azadirachta indica, dried seeds of Sesamum indicum and Bees' honey which is clinically use for open wounds. The current study was designed as a microbiological assay and the key objective was to evaluate the antibacterial efficacy of TNK against laboratory specimen of Staphylococcus aureus (ATCC25923). The Anti-Bacterial Sensitivity Test was conducted according to the Kirby Bauer method using Agar Well Diffusion method by comparing the effect of Amoxicillin as the positive controller and distilled water as the negative controller in triplicates. The testing drug was assessed as D1 and D2 in 1:2 concentration ratios respectively. Results of the study were obtained through the diameter measurement of inhibitory zone and assessed using one -sample Ttest. D1 depicted p value as 0.024 and Ttest was 4.44. D2 depicted p value as 0.100 and T-test was 1.89. Comparing to the positive control drug, the hypothesis was generated as H 0 : <=19 mm and H 1 : >19 mm. Referring to hypothesis, H 0 of D1 was rejected and H 0 of D2 was not rejected. The significant level was considered less than 5%. D2 concentration of TNK was significant against laboratory specimen of Staphylococcus aureus. The study suggests that the TNK is consisted with an extrinsic effective antibacterial application for infected wounds which were caused by Staphylococcus aureus. Further clinical study on human subjects will verify the efficacy of TNK in clinical manifestations.