Antimicrobial efficacy of different endodontic sealers against Enterococcus faecalis: An In vitro study

Abstract

ObjectiveThe aim of this in vitro study is to compare the antimicrobial efficacy of four different endodontic sealers against Enterococcus faecalis.Material and Method:Four different endodontic sealers, namely, resin based (AH Plus), zinc oxide-eugenol based (Tubliseal), calcium hydroxide based (Sealapex), and mineral trioxide aggregate (MTA Fillapex) based were tested for their antimicrobial efficacy against E. faecalis using agar diffusion method. Four wells were made by the removal of agar at equidistant points and filled with freshly mixed respective root canal sealers and were inoculated with E. faecalis. All the three plates were incubated for a period of 72 h at 37°C under aerobic conditions. The diameter of inhibition zones was measured at 24, 48, and 72 h time intervals. Data obtained were statistically analyzed using one-way analysis of variance and unpaired t-test.Results:All the tested sealers showed some bacterial growth inhibition of E. faecalis. Their efficacy in descending order of antibacterial activity was as follows: Sealapex > AH Plus > Tubliseal > MTA Fillapex. The efficacy of the root canal sealers decreased marginally with increase in their duration of action.Conclusion:Antimicrobial efficacy of calcium hydroxide-based sealer was highest followed by resin-based sealer and was the least with MTA based sealer.

<p>The Antibiofilm Activity and Mechanism of Nanosilver- and Nanozinc-Incorporated Mesoporous Calcium-Silicate Nanoparticles</p>

“…28 However, MTA has no obvious antibacterial activity and cannot effectively kill E. faecalis, S. aureus, E. coli or other anaerobic bacteria, and it is difficult to enter the narrow and curved root canal and dentin tubules to remove the bacteria. 29 Pompermayer et al found that MTA was not effective against multispecies microcosm biofilms including E. faecalis, Streptococcus salivarius (S. salivarius), Streptococcus mutans (S. mutans) and so on. 30 In this study, the E. faecalis biofilms treated with MTA was still intact, and the proportion of live bacteria was high, suggesting that MTA had no obvious antibacterial effect on E. faecalis biofilms, which was similar to previous studies.…”

Antimicrobial efficacy of commercially available endodontic bioceramic root canal sealers: A systematic review

“…Thus, data about the antimicrobial efficacy of each group could not be extracted precisely. We contacted the corresponding authors in cases when additional data were required [27,44–53], but most of them did not reply after the second email. One author [47] replied and wrote that disclosure of raw data is against their policy.…”

“…The most commonly used antimicrobial test was the agar diffusion test (ADT) [33,34,38,45,48,50,52,53,59–61]. Nine studies used the direct contact test (DCT) [16,36,40,46,50,56–58,62], where colony forming units (CFUs) were counted.…”

Cimentos biocerâmicos em endodontia: revisão integrativa

“…Esses materiais possuem diferentes propriedades e composições, como a atividade antimicrobiana observada nos cimentos à base de cimentos de óxido de zinco e eugenol e a base de hidróxido de cálcio, e a adesão, nos cimentos à base de cimentos ionômeros de vidro e resina. No entanto, não existe um único cimento que contemple todas as propriedades desejadas (Dalmia et al, 2018).…”