Antimicrobial efficacy and inactivation kinetics of a novel LED-based UV-irradiation technology

Schöbel, Harald; Diem, Gabriel; Kiechl, Julia; Chistè, Daniela; Bertacchi, Giulia; Mayr, A; Wilflingseder, Doris; Lass‐Flörl, Cornelia; Posch, Wilfried

doi:10.1016/j.jhin.2022.12.023

Cited by 8 publications

(3 citation statements)

References 38 publications

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“…Although UV-C disinfection shows effectiveness against some pathogens, it can cause bacterial mutations ( 68 ). A new, LED-based UV-irradiation technology has shown to be effective against some bacteria and viruses, but it is connected to high costs ( 69 ), which is uneconomical for use in public transport.…”

Section: Introduction and Discussionmentioning

confidence: 99%

An overview of the bacterial microbiome of public transportation systems—risks, detection, and countermeasures

Ly,

Leuko,

Moeller

2024

Front. Public Health

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When we humans travel, our microorganisms come along. These can be harmless but also pathogenic, and are spread by touching surfaces or breathing aerosols in the passenger cabins. As the pandemic with SARS-CoV-2 has shown, those environments display a risk for infection transmission. For a risk reduction, countermeasures such as wearing face masks and distancing were applied in many places, yet had a significant social impact. Nevertheless, the next pandemic will come and additional countermeasures that contribute to the risk reduction are needed to keep commuters safe and reduce the spread of microorganisms and pathogens, but also have as little impact as possible on the daily lives of commuters. This review describes the bacterial microbiome of subways around the world, which is mainly characterized by human-associated genera. We emphasize on healthcare-associated ESKAPE pathogens within public transport, introduce state-of-the art methods to detect common microbes and potential pathogens such as LAMP and next-generation sequencing. Further, we describe and discuss possible countermeasures that could be deployed in public transportation systems, as antimicrobial surfaces or air sterilization using plasma. Commuting in public transport can harbor risks of infection. Improving the safety of travelers can be achieved by effective detection methods, microbial reduction systems, but importantly by hand hygiene and common-sense hygiene guidelines.

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Section: Introduction and Discussionmentioning

confidence: 99%

An overview of the bacterial microbiome of public transportation systems—risks, detection, and countermeasures

Ly,

Leuko,

Moeller

2024

Front. Public Health

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“…Given the limited existing research on P. fragi, particularly regarding UV-C inactivation on surfaces, we referred to literature data to closely related strains. A study reported UV-C inactivation of Pseudomonas uorescens using 275 and 285 nm LEDs on stainless steel surfaces 45 . They found D 90 values of 2.3 and 1.5 mJ.cm − 2 for 275 and 285 nm UV-C LEDs, respectively, achieving a 4 log 10 reduction at a 6 mJ.cm − 2 UV-C dose using 285 nm LEDs.…”

Section: Uv-c Of Salmonella Enteritismentioning

confidence: 99%

Inactivation of Deposited Bioaerosols on Food Contact Surfaces with UV-C Light Emitting Diode Devices

Sharma,

Singh,

Pendyala

et al. 2024

Preprint

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The airborne transmission of infectious diseases and bioaerosol-induced cross-contamination pose significant challenges in the food, dairy, and pharma industries. This study evaluated the effectiveness of 279 nm UV-C LED irradiation for decontaminating bioaerosols, specifically containing microorganisms like E. coli (C3040- Kanamycin resistant), Salmonella Enteritis (ATCC 4931), and Pseudomonas fragi (ATCC 4973), on food contact surfaces. Borosilicate glass, silicon rubber, and stainless steel (316L) surfaces were selected for experimentation for their usage in the food industry. A 50µL cell suspension was aerosolized at 25 psi pressure using a 4-jet BLAM Nebulizer within a customized glass chamber and then deposited onto the surface of the coupons. The serial dilution approach was used for the microbial enumeration, followed by double plating. With a low RMSE and high R2 values, the biphasic kinetic model demonstrated the excellent goodness of fit parameters. At a UV-C dose of 6 mJ cm− 2, glass surfaces showed the maximum microbial inactivation ( i.e. 2.80, 3.81, and 3.56 log CFU/mL for E. coli, Salmonella, and P. fragi, respectively). Stainless steel and silicon rubber showed significant microbial inactivation but were consistently lower than glass. Our research indicates that UV-C LEDs can disinfect bioaerosols on food contact surfaces effectively.

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“…In particular, a shorter UV wavelength has been shown to have a higher bactericidal effect, e.g. it has been found that UV-C treatment with 255-265 nm can achieve a higher level of bacterial inactivation than 275-285 nm (Li et al 2017;Schöbel et al 2023). However, there is still a need to evaluate these novel physical disinfection methods on chicken transport crates.…”

Section: Cleaning and Disinfection Of Chicken Transport Cratesmentioning

confidence: 99%

Foodborne bacteria in slaughterhouses : with focus on cleaning and disinfection

Moazzami

2023

Acta Universitatis Agriculturae Sueciae

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Proper cleaning and disinfection (C&D) in slaughterhouses and meat processing premises is a prerequisite for production of safe, high-quality meat. An example of how inadequate C&D can affect human health is provided by the strong increase in number of human cases of campylobacteriosis in Sweden in 2016-2017 due to insufficiently cleaned transport crates for chicken. This thesis evaluated the efficacy of C&D procedures in slaughterhouses and adjacent meat processing premises by testing for pathogenic and hygiene indicator bacteria on equipment, facilities and carcasses. Different sampling methods used for assessing surface cleanliness were compared and slaughterhouses with associated meat processing premises in Sweden were interviewed regarding their C&D routines and monitoring activities. The results indicated that the C&D procedures used for surfaces in the slaughterhouse premises were sufficient regarding removal/inactivation of pathogenic bacteria. However, only approximately half of the surfaces were assessed as sufficiently cleaned, as the amount of hygiene indicator bacteria was high. Campylobacter jejuni, C. coli and Listeria monocytogenes were detected on food contact surfaces, indicating high risk of contamination of carcasses and meat. The same L. monocytogenes strain was found in a red meat slaughterhouse environment on three occasions 15 weeks apart, while a C. jejuni strain showed potential to spread within a poultry slaughterhouse. Extended-spectrum beta-lactamase (ESBL) Escherichia coli was not detected. New technologies such as 265 nm UV-C LED irradiation on chicken transport crates and ultrasound-steam treatment and chilling of broiler carcasses were shown to give significant bacterial reductions. However, these techniques require a certain time of action that is difficult to combine with high slaughter speed in an industrial setting. Dipslides and ATP-bioluminescence were found to be of varying reliability, but are commonly used by Swedish slaughterhouses with associated meat processing premises.

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Antimicrobial efficacy and inactivation kinetics of a novel LED-based UV-irradiation technology

Cited by 8 publications

References 38 publications

An overview of the bacterial microbiome of public transportation systems—risks, detection, and countermeasures

An overview of the bacterial microbiome of public transportation systems—risks, detection, and countermeasures

Inactivation of Deposited Bioaerosols on Food Contact Surfaces with UV-C Light Emitting Diode Devices

Foodborne bacteria in slaughterhouses : with focus on cleaning and disinfection

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