Antimicrobial Effects of Pressured Carbon Dioxide in a Continuous Flow System

Shimoda, Mitsuya; Yamamoto, Yasuhiko; Cocunubo-Castellanos, J.; Tonoike, Hiroshi; Kawano, Teruhiko; Ishikawa, Hiroya; Osajima, Yutaka

doi:10.1111/j.1365-2621.1998.tb15819.x

Cited by 80 publications

(52 citation statements)

References 16 publications

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“…4). We have reported that pressure influenced the inactivation of enzymes on batch treatment, 10) but it was found that the concentration of CO 2 dissolved in the sample during continuous flow treatment 15,16) was higher than that during batch treatment 10) at the same pressure. Therefore, such inconsistency can be attributed to the difference in the mode of treatment.…”

Section: Effects Of Co 2 Feeding Ratio and Pressure On Inactivation Omentioning

confidence: 99%

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Inactivation of Enzymes in Fresh Sake Using a Continuous Flow System for High-Pressure Carbonation

Tanimoto

Matsumoto²,

Fujii³

et al. 2005

Bioscience, Biotechnology, and Biochemistry

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Section: Effects Of Co 2 Feeding Ratio and Pressure On Inactivation Omentioning

confidence: 99%

“…Furthermore, we developed a new apparatus for a continuous flow system with microbubbles of pressurized CO 2 . -Amylase (AA) and acid protease 14) were efficiently inactivated in a saline, and L. brevis and S. cerevisiae 15,16) were also effectively inactivated by this system.…”

mentioning

confidence: 95%

Inactivation of Enzymes in Fresh Sake Using a Continuous Flow System for High-Pressure Carbonation

Tanimoto

Matsumoto²,

Fujii³

et al. 2005

Bioscience, Biotechnology, and Biochemistry

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“…Physical changes have been reported on vegetative bacteria and fungi killed by SCCO 2 . Cell wall rupture has been observed with E. coli (Fraser, 1951) and S. cerevisiae (Shimoda et al, 1998), resulting from fast expansion of absorbed CO 2 during the depressurization stage. Changes of internal structures, such as gas between the cell wall and cytoplasm, fractures in cell membranes, were observed on L. plantarum with SEM (Hong and Pyun, 1999).…”

Section: Introductionmentioning

confidence: 99%

Supercritical carbon dioxide and hydrogen peroxide cause mild changes in spore structures associated with high killing rate of Bacillus anthracis

Zhang¹,

Dalal²,

Matthews³

et al. 2007

Journal of Microbiological Methods

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The present work examines chemical and structural response in B. anthracis spores killed by a mixture of supercritical carbon dioxide (SCCO 2 ) and hydrogen peroxide (H 2 O 2 ). Deactivation of 6-log of B. anthracis spores by SCCO 2 +H 2 O 2 was demonstrated, but changes in structure were observed in only a small portion of spores. Results from phase contrast microscopy proved that this treatment is mild and does not trigger germination-like changes. TEM imaging revealed mild damage in a portion of spores while the majority remained intact. Dipicolinic acid (DPA) analysis showed that <10% of the DPA was released from the spore core into the external milieu, further demonstrating only modest damage to the spores. Confocal fluorescent microscopy, assessing uptake of DNAbinding dyes, directly demonstrated compromise of the permeability barrier. However, the magnitude of uptake was small compared to spores that had been autoclaved. This work suggests that SCCO 2 +H 2 O 2 is quite mild compared to other sterilization methods, which has major implications in its application. These results provide some insight on the possible interactions between spores and the SCCO 2 +H 2 O 2 sterilization process.

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“…The inoculated media was immediately placed in a 4 L pressure vessel (Alloy Products Corp, Waukesha, WI) and made anaerobic through the use of a GasPak ™ EZ Anaerobe Container System Sachet (BD, Franklin Lakes, NJ).The serum bottles in the vessels were then pressurized to 1.0 MPa in the field with ultrapure CO 2 . Previous work has shown that CO 2 is very effective sterilant (Nakamura et al, 1994;Isenschmid et al, 1995;Ballestra et al, 1996;Shimoda et al, 1998;Hong and Pyun, 1999;Erkmen, 2000;Spilimbergo and Bertucco, 2003;Watanabe et al, 2003;Damar and Balaban, 2006;Oule et al, 2006Oule et al, , 2010Song et al, 2007). With this in mind, we reasoned that cultures grown at very high CO 2 pressures would likely result in a sterilization of most viable microorganisms, especially those accidentally introduced through contamination.…”

Section: Field Samplingmentioning

confidence: 99%

Isolation and characterization of a CO2-tolerant Lactobacillus strain from Crystal Geyser, Utah, U.S.A.

et al. 2015

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When CO 2 is sequestered into the deep subsurface, changes to the subsurface microbial community will occur. Capnophiles, microorganisms that grow in CO 2 -rich environments, are some organisms that may be selected for under the new environmental conditions. To determine whether capnophiles comprise an important part of CO 2 -rich environments, an isolate from Crystal Geyser, Utah, U.S.A., a CO 2 -rich spring considered a carbon sequestration analog, was characterized. The isolate was cultured under varying CO 2 , pH, salinity, and temperature, as well as different carbon substrates and terminal electron acceptors (TEAs) to elucidate growth conditions and metabolic activity. Designated CG-1, the isolate is related (99%) to Lactobacillus casei in 16S rRNA gene identity, growing at PCO 2 between 0 and 1.0 MPa. Growth is inhibited at 2.5 MPa, but stationary phase cultures exposed to this pressure survive beyond 5 days. At 5.0 MPa, survival is at least 24 h. CG-1 grows in neutral pH, 0.25 M NaCl, and between 25 • and 45 • C and consumes glucose, lactose, sucrose, or crude oil, likely performing lactic acid fermentation. Fatty acid profiles between 0.1 and 1.0 MPa suggests decreases in cell size and increases in membrane rigidity. Transmission electron microscopy reveals rod shaped bacteria at 0.1 MPa. At 1.0 MPa, cells are smaller, amorphous, and produce abundant capsular material. Its ability to grow in environments regardless of the presence of CO 2 suggests we have isolated an organism that is more capnotolerant than capnophilic. Results also show that microorganisms are capable of surviving the stressful conditions created by the introduction of CO 2 for sequestration. Furthermore, our ability to culture an environmental isolate indicates that organisms found in CO 2 environments from previous genomic and metagenomics studies are viable, metabolizing, and potentially affecting the surrounding environment.

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Antimicrobial Effects of Pressured Carbon Dioxide in a Continuous Flow System

Cited by 80 publications

References 16 publications

Inactivation of Enzymes in Fresh Sake Using a Continuous Flow System for High-Pressure Carbonation

Inactivation of Enzymes in Fresh Sake Using a Continuous Flow System for High-Pressure Carbonation

Supercritical carbon dioxide and hydrogen peroxide cause mild changes in spore structures associated with high killing rate of Bacillus anthracis

Isolation and characterization of a CO2-tolerant Lactobacillus strain from Crystal Geyser, Utah, U.S.A.

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