Antimicrobial Effects of Platelet-rich Plasma against Selected Oral and Periodontal Pathogens

Tekin, Nilgün Solak; Cihan, Arzu Çöleri; Karaca, Başar; Çökmüş, Cumhur

doi:10.5604/17331331.1234992

Cited by 6 publications

(4 citation statements)

References 58 publications

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“…Among these studies, Mala and Srividya (2010) reported isolating two different isoenzymes with molecular weights of 66 and 18 KDa from a Bacillus species. Tekin et al (2012) reported similar results, isolating alkaline proteases from Bacillus cohnii APT5.…”

Section: Purification Of the Protease Enzymesupporting

confidence: 60%

Production, purification and characterization of a thermostable alkaline serine protease from Bacillus lichniformis NMS-1

Mathew¹,

Gunathilaka²

2015

Int. J. Biotechnol. Mol. Biol. Res.

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Alkaline proteases are used in food industry, leather tanning and processing industry, preparation of pharmaceuticals and also in the fiber industry. An alkaline serine protease producing strain was isolated using soil sample from a natural hot water spring in Sri Lanka. It was identified based on morphological, biochemical and 16s rRNA identifications as Bacillus licheniformis NMS-1. The extacellular protease enzyme was purified by two steps procedure involving ammonium sulfate precipitation followed by DEAE-Sephadex A-25 gel chromatography. The purification gave a 56 fold increase of the specific activity with a yield of 16%. The optimal pH and optimal temperature of the protease were pH 9 and 60C, respectively. The protease was relatively stable between 20-80°C. The enzyme was stable within the pH values of 8-12. The K m and V max values calculated from Lineweaver-Burk plot were 2.7x10-3 mg/ml and 263 mU/mg. Among the protease inhibitors that were tested, PMSF completely inhibited the enzyme activity indicating that the protease is a serine protease. The enzyme retained more than 50% of its activity after 60 min incubation at 60C. The major protease types used commercially are heat stable alkaline proteases. Alkaline serine proteases are enzymes that cleave peptide bonds in protein in which serine serves as the nucleophilic amino acid at the enzyme active site. Properties of this protease have shown it's suitability for industrial applications such as detergent industry.

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Section: Purification Of the Protease Enzymesupporting

confidence: 60%

Production, purification and characterization of a thermostable alkaline serine protease from Bacillus lichniformis NMS-1

Mathew¹,

Gunathilaka²

2015

Int. J. Biotechnol. Mol. Biol. Res.

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“…PRF can be activated immediately by endogenous thrombin during centrifugation process. The limitation use of bovine thrombin since its restriction from the routine use because of the major risk of coagulopathy from antibody formation [12]. The use of CaCl 2 and thrombin to coagulate the platelets into a gel form and activate the containing GF initiate a burst release and activation of all the GF's of PRP all at once, therefore, the duration of action of PRP is short.…”

Section: Discussionmentioning

confidence: 99%

Locally Inhibition of Orthodontic Relapse by Injection of Carbonated Hydroxy Apatite-Advanced Platelet Rich Fibrin in a Rabbit Model

Alhasyimi

Pudyani

Asmara

et al. 2017

KEM

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Relapse is considered a significant failure after orthodontic treatment. In response to relapse, RANKL expressions will increase, while OPG expressions will decrease. CHA is thought to be one of an ideal candidate for enhancing bone formation. Moreover, a-PRF is a source high levels of growth factors that play a central role in the bone remodeling. This research was intended to investigate the effect of hydrogel CHA-aPRF in preventing relapse. Hydrogel-CHA was initially designed, with its degradation profile and FTIR (Fourie’s Transform Infrared) spectra were investigated as the basis to find out optimum formulation before incorporated with aPRF. Hydrogel-CHA microspheres were prepared in 3 different compositions: those were encoded 30-CHA, 40-CHA, and 50-CHA. After the hydrogel formulation and characterization were completed, 10 mL blood samples were collected, then centrifuged at 1500 rpm for 14 min. At the end of the centrifugation process, the aPRF clot was isolated and then pressed to obtain their releasate. The releasate aPRF was then loaded into the best formulation candidate of hydrogel CHA. The hydrogel incorporated aPRF was then gently injected on the mesial side of incisor gingival sulcus of the rabbit after orthodontic tooth movement. The FTIR analysis showed that carbonated apatite was successfully developed during the fabrication process of hydrogel-CHA microspheres. It was also known that degradation profile of 30-CHA was considered ideal compared to the other compositions. The application of CHA-aPRF (group C) was proven to significantly prevent relapse, indicated by lowest percentage of relapse 21 days after debonding (29.95±3.91%) compared to control group. Furthermore, it has been found that expressions of RANKL were significantly lowest (p<0.05) in group C on day 0, 3, and 7, while OPG expressions showed significantly highest (p<0.05) in group C on day 14 and 21 after debonding. These results indicate that incorporation of hydrogel-CHA has potential effect to enhance alveolar bone remodeling and prevent orthodontic relapse by stimulates OPG expression and suppresses RANKL expression.

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“…Endogenous thrombin, released during centrifugation, can quickly activate PRF. The restriction on the usual use of bovine thrombin due to the high risk of coagulopathy from antibody development [ 31 ] has limited its use. The use of calcium chloride and thrombin to coagulate platelets into a gel and engage the contained GF initiates a burst release and activation of all the GFs of PRP simultaneously; thus, the period of action of PRP is brief.…”

Section: Application Of Hydrogel Carbonated Hydroxyapatite-incorporat...mentioning

confidence: 99%

The Use of Polymers to Enhance Post-Orthodontic Tooth Stability

2022

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Relapse after orthodontic treatment occurs at a rate of about 70 to 90%, and this phenomenon is an orthodontic issue that has not yet been resolved. Retention devices are one attempt at prevention, but they require a considerable amount of time. Most orthodontists continue to find it challenging to manage orthodontic relapse; therefore, additional research is required. In line with existing knowledge regarding the biological basis of relapse, biomedical engineering approaches to relapse regulation show promise. With so many possible uses in biomedical engineering, polymeric materials have long been at the forefront of the materials world. Orthodontics is an emerging field, and scientists are paying a great deal of attention to polymers because of their potential applications in this area. In recent years, the controlled release of bisphosphonate risedronate using a topically applied gelatin hydrogel has been demonstrated to be effective in reducing relapse. Simvastatin encapsulation in exosomes generated from periodontal ligament stem cells can promote simvastatin solubility and increase the inhibitory action of orthodontic relapse. Moreover, the local injection of epigallocatechin gallate-modified gelatin suppresses osteoclastogenesis and could be developed as a novel treatment method to modify tooth movement and inhibit orthodontic relapse. Furthermore, the intrasulcular administration of hydrogel carbonated hydroxyapatite-incorporated advanced platelet-rich fibrin has been shown to minimize orthodontic relapse. The objective of this review was to provide an overview of the use of polymer materials to reduce post-orthodontic relapse. The most promising polymeric materials and their potential orthodontic uses for the prevention of orthodontic relapse are also discussed.

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Antimicrobial Effects of Platelet-rich Plasma against Selected Oral and Periodontal Pathogens

Cited by 6 publications

References 58 publications

Production, purification and characterization of a thermostable alkaline serine protease from Bacillus lichniformis NMS-1

Production, purification and characterization of a thermostable alkaline serine protease from Bacillus lichniformis NMS-1

Locally Inhibition of Orthodontic Relapse by Injection of Carbonated Hydroxy Apatite-Advanced Platelet Rich Fibrin in a Rabbit Model

The Use of Polymers to Enhance Post-Orthodontic Tooth Stability

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