Antimicrobial Effect of Triclosan and Triclosan with Gantrez on Five Common Endodontic Pathogens

Nudera, William J.; Fayad, Mohamed I.; Johnson, Bradford R.; Zhu, Min; Wenckus, Christopher S.; BeGole, Ellen A.; Wu, Christine D.

doi:10.1016/j.joen.2007.06.009

Cited by 37 publications

(30 citation statements)

References 20 publications

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“…The MIC and minimum bactericidal concentration (MBC) of EGCg against S. mutans UA159 were determined by a microdilution method in both CDM and BHI, as described previously (45).…”

Section: Methodsmentioning

confidence: 99%

“…The use of different culture media and growth conditions has also contributed to the variable antimicrobial effects of tea polyphenols. The in vitro investigations into the effects of tea polyphenols on cariogenic virulence factors have focused on GTFs activity, EPS production, and cell adherence (26,41,45,51). We hypothesized that the major antimicrobial monomeric component of tea polyphenols, epigallocatechin gallate (EGCg), contributes to the suppression of virulence factors of S. mutans other than GTFs.…”

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confidence: 99%

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The Tea Catechin Epigallocatechin Gallate Suppresses Cariogenic Virulence Factors of Streptococcus mutans

Zhou

2011

Antimicrob Agents Chemother

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269

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Streptococcus mutans, the primary etiologic agent of dental caries, possesses a series of virulence factors associated with its cariogenicity. Alternatives to traditional antimicrobial treatment, agents selectively inhibiting the virulence factors without necessarily suppressing the resident oral species, are promising. The anticariogenic properties of tea have been suggested in experimental animals and humans. Tea polyphenols, especially epigallocatechin gallate (EGCg), have been shown to inhibit the growth and glucosyltransferases activity of S. mutans. However, their effects on biofilm and cariogenic virulence factors of oral streptococci other than glucosyltransferases have not been well documented. In this study, we investigated the biological effect of EGCg on the virulence factors of S. mutans associated with its acidogenicity and acidurity. The antimicrobial effects of EGCg on S. mutans biofilm grown in chemically defined medium were also examined. EGCg inhibited growth of S. mutans planktonic cells at an MIC of 31.25 g/ml and a minimal bactericidal concentration (MBC) of 62.5 g/ml. EGCg also inhibited S. mutans biofilm formation at 15.6 g/ml (minimum concentration that showed at least 90% inhibition of biofilm formation) and reduced viability of the preformed biofilm at 625 g/ml (sessile MIC 80 ). EGCg at sub-MIC levels inhibited acidogenicity and acidurity of S. mutans cells. Analysis of the data obtained from real-time PCR showed that EGCg significantly suppressed the ldh, eno, atpD, and aguD genes of S. mutans UA159. Inhibition of the enzymatic activity of F 1 F o -ATPase and lactate dehydrogenase was also noted (50% inhibitory concentration between 15.6 and 31.25 g/ml). These findings suggest that EGCg is a natural anticariogenic agent in that it exhibits antimicrobial activity against S. mutans and suppresses the specific virulence factors associated with its cariogenicity.

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“…The MIC and minimum bactericidal concentration (MBC) of EGCg against S. mutans UA159 were determined by a microdilution method in both CDM and BHI, as described previously (45).…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

The Tea Catechin Epigallocatechin Gallate Suppresses Cariogenic Virulence Factors of Streptococcus mutans

Zhou

2011

Antimicrob Agents Chemother

Self Cite

269

227

View full text Add to dashboard Cite

show abstract

“…Minimal inhibitory concentrations (MICs) were determined using a microdilution method in microtiter plates (24). Test bacteria included E. faecalis American Type Culture Collection (ATCC) 29212, P. intermedia ATCC 25611, and F. nucleatum ATCC 10953.…”

Section: Antibacterial Activity and Bacterial Growth Assaymentioning

confidence: 99%

Efficacy of Berberine, an Antimicrobial Plant Alkaloid, as an Endodontic Irrigant against a Mixed-culture Biofilm in an In Vitro Tooth Model

Xie

Johnson

Wenckus

et al. 2012

Journal of Endodontics

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“…69 The PVM-MA copolymer signifi cantly lowers the minimum inhibitory concentration of triclosan necessary to kill aerobic and anaerobic periodontal, endodontic and salivary pathogens. 13,76 In the oral cavity, the PVM-MA copolymer ingredient in toothpaste acts as a superior oral bioadhesive controlledrelease delivery system for triclosan. 13,75,77 It enhances triclosan reservoirs on the buccal mucosa, on hard tooth surfaces and in saliva.…”

Section: The Role Of the Copolymermentioning

confidence: 99%

Is there a role for triclosan/copolymer toothpaste in the management of periodontal disease?

et al. 2009

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Antimicrobial Effect of Triclosan and Triclosan with Gantrez on Five Common Endodontic Pathogens

Cited by 37 publications

References 20 publications

The Tea Catechin Epigallocatechin Gallate Suppresses Cariogenic Virulence Factors of Streptococcus mutans

The Tea Catechin Epigallocatechin Gallate Suppresses Cariogenic Virulence Factors of Streptococcus mutans

Efficacy of Berberine, an Antimicrobial Plant Alkaloid, as an Endodontic Irrigant against a Mixed-culture Biofilm in an In Vitro Tooth Model

Is there a role for triclosan/copolymer toothpaste in the management of periodontal disease?

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