Antimicrobial Effect of Ozonated Water on Bacteria Invading Dentinal Tubules

Abstract

Ozone is known to act as a strong antimicrobial agent against bacteria, fungi, and viruses. In the present study, we examined the effect of ozonated water against Enterococcus faecalis and Streptcoccus mutans infections in vitro in bovine dentin. After irrigation with ozonated water, the viability of E. faecalis and S. mutans invading dentinal tubules significantly decreased. Notably, when the specimen was irrigated with sonication, ozonated water had nearly the same antimicrobial activity as 2.5% sodium hypochlorite (NaOCl). We also compared the cytotoxicity against L-929 mouse fibroblasts between ozonated water and NaOCl. The metabolic activity of fibroblasts was high when the cells were treated with ozonated water, whereas that of fibroblasts significantly decreased when the cells were treated with 2.5% NaOCl. These results suggest that ozonated water application may be useful for endodontic therapy.

The antimicrobial effects of root canal irrigation and medication

“…Ozone is known to act as a strong antimicrobial agent 78 and has been shown to effectively disinfect bovine dentinal tubules in vivo. 79 These developments in the field of root canal disinfection provide encouraging results for future development of novel irrigation materials and techniques that can be used instead of or as adjunct to the conventional materials currently in use.…”

Ozone Gas Effect on Mineral Content of Dentin exposed to Streptococcus mutans Biofilm: An Energy-dispersive X-ray Evaluation

“…Nagayoshi et al 23 found that ozonated water was effective against Gram-positive and Gramnegative oral microorganisms and oral Candida albicans in pure culture. Ozonated water also had strong bactericidal activity against the bacteria in biofilm.…”

Enterococcus faecalis, a nightmare to endodontist: A systematic review

“…Shur et al (2003) chlorhexidine-impregnated and iodoform-containing gutta-percha points have shown little inhibitory action against E. faecalis. In Nagayoshi et al (2004), ozonated water has been shown to have the same antimicrobial efficacy as 2.5% sodium Hypochlorite. In , the antimicrobial activity against E. faecalis of various sealers has also been studied.…”