Antimicrobial effect of lauroyl arginate ethyl on Escherichia coli O157:H7 and Listeria monocytogenes on red oak leaf lettuce

Nübling, Simone; Wohlt, Daria; Saile, Nadja; Weiß, Agnes; Schmidt, Herbert

doi:10.1007/s00217-016-2802-1

Cited by 12 publications

(14 citation statements)

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“…Upon deletion of hcpA decreased adherence was observed on spinach leaves (Saldana et al, 2011). Interestingly, its deletion resulted in enhanced attachment to red oak leaf lettuce leaves (Nuebling et al, 2017). As these two studies used different incubation periods, the obtained results may indicate that adherence is…”

Section: Accepted Manuscriptmentioning

confidence: 95%

“…The hcpA gene encodes the pilin subunit of an adhesive type IV pilus called hemorrhagic coli pilus (HCP) (Xicohtencatl-Cortes et al, 2007) and was formerly called prepilin peptidasedependent gene (ppdD) (Ledesma et al, 2010). The hemorrhagic coli pilus was shown to be involved in adherence to a variety of mammalian cell lines (Xicohtencatl-Cortes et al, 2007), and to leaf surfaces (Nuebling et al, 2017;Saldana et al, 2011). However, contradicting results were gained when investigating attachment to leaf surfaces.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…PCR products for deleting the target genes carry the kanamycin resistance gene flanked by FRT sites and 40 -50 nt homologous to the gene of interest. For the deletion of hcpA, the primer pair G70 hcpA-P1 and G71 hcpA-P2 was used as reported by Nuebling et al (2017), and for iha primers deliha-for and deliha-rev were used (Table 2). In case of successful mutagenesis, application of these primer pairs led to deletion of 78 % and 93 % of hcpA and iha, respectively.…”

Section: Construction Of Isogenic Deletion Mutantsmentioning

confidence: 99%

“…Several studies have already demonstrated that E. coli O157:H7 strains are able to colonize the leaves and roots of lettuce and other leafy greens (Erickson et al, 2010;Seo and Frank, 1999;Solomon et al, 2002), and to persist for several days to weeks (Chitarra et al, 2014;Wright et al, 2017). Surface structures such as pili, flagella, the type III secretion system (T3SS), as well as proteins involved in quorum sensing were found to be involved in successful adherence to spinach leaves and leaves of red oak lettuce (Macarisin et al, 2012;Nuebling et al, 2017;Saldana et al, 2011) under laboratory conditions.…”

Section: Introductionmentioning

confidence: 99%

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Adherence factors of enterohemorrhagic Escherichia coli O157:H7 strain Sakai influence its uptake into the roots of Valerianella locusta grown in soil

et al. 2018

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Increasing numbers of outbreaks caused by enterohemorrhagic Escherichia coli (EHEC) are associated with the consumption of contaminated fresh produce. The contamination of the plants may occur directly on the field via irrigation water, surface water, manure or fecal contamination. Suggesting a low infectious dose of 10 to 10 cells, internalization of EHEC into plant tissue presents a serious public health threat. Therefore, the ability of EHEC O157:H7 strain Sakai to adhere to and internalize into root tissues of the lamb's lettuce Valerianella locusta was investigated under the environmental conditions of a greenhouse. Moreover, the influence of the two adherence and colonization associated genes hcpA and iha was surveyed regarding their role for attachment and invasion. Upon soil contamination, the number of root-internalized cells of EHEC O157:H7 strain Sakai exceeded 10 cfu/g roots. Deletion of one or both of the adherence factor genes did not alter the overall attachment of EHEC O157:H7 strain Sakai to the roots, but significantly reduced the numbers of internalized bacteria by a factor of between 10 and 30, indicating their importance for invasion of EHEC O157:H7 strain Sakai into plant roots. This study identified intrinsic bacterial factors that play a crucial role during the internalization of EHEC O157:H7 strain Sakai into the roots of Valerianella locusta grown under the growth conditions in a greenhouse.

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Section: Accepted Manuscriptmentioning

confidence: 95%

Section: Accepted Manuscriptmentioning

confidence: 99%

Section: Construction Of Isogenic Deletion Mutantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Adherence factors of enterohemorrhagic Escherichia coli O157:H7 strain Sakai influence its uptake into the roots of Valerianella locusta grown in soil

et al. 2018

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“…Additionally, Nübling et al (2017) used LAE to reduce the count of pathogenic bacteria Escherichia coli O157:H7 and Listeria monocytogenes in lettuce, and it was found that LAE was able to decrease the count of these bacteria in this vegetable.…”

Section: Antimicrobial Effect Of Lae When Applied To Foodmentioning

confidence: 99%

Nα-lauroyl-l-arginine ethyl ester monohydrochloride, an antimicrobial agent and its use: a review

Motta

Ribeiro-Santos

Guimarães

et al. 2020

RSD

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Growing demand for safe foods coupled with the intent to reduce food waste, seeing as much of it is lost through contamination by spoilage microorganisms, leads to research on antimicrobial agents such as LAE (Nα-lauroyl-L-arginine ethyl ester monohydrochloride). This compound has great antimicrobial potential against a range of microorganisms and, therefore, its use may be of extreme importance for the food industry in the search for antimicrobial agents with a broad spectrum of action. Thus, the objective of this article is to review the research involving LAE, when studied in vitro, in vivo and in the incorporation in different packaging in order to be released in a controlled manner for food products. In conclusion, despite the fact that it has a strong antimicrobial activity, it is still little known and is not accepted in all countries, including Brazil. With greater insight into this antimicrobial agent, more countries could use it, supporting worldwide in food preservation.

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Mechanism of fungal inhibition activity of Nα‐lauroyl‐L‐arginine ethyl ester (LAE) and potential in control of Penicillium expansum on postharvest citrus ‘Benimadonna’ (Citrus reticulata × Citrus sinensis)

2022

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BACKGROUND: Citrus 'Benimadonna' (Citrus reticulata × Citrus sinensis) is a high-value perishable fruit; thus there is an urgent need for a preservation technology with high effectiveness and low safety risk from industries. N⊍-Lauroyl-L-arginine ethyl ester hydrochloride (LAE) was applied to enhance preservability by compounding with natamycin, and a possible fungal inhibition mechanism based on the hypothesis of an impact on the cell membrane by surfactant was investigated. RESULTS:In vitro testing showed that the minimum inhibitory concentration of LAE against Penicillium expansum (PE), isolated as the predominant spoilage-inducing fungus, was 32 mg L −1 and it was partially synergistic with natamycin. Subsequent in vivo testing proved the inhibition capacity. During 90 days' refrigerated preservation, spoilage rate was significantly decreased by preharvest spraying versus control without extra taste loss, and LAE showed an alleviating benefit on total pectin loss. Subsequently, electron microscopic imaging and intracellular protein levels of PE exposed to LAE indicated that LAE stress led to increased permeability and decreased cell integrity. Moreover, peroxidase, superoxide dismutase and catalase revealed that LAE enhanced oxidative stress, while pectinase was antagonized.CONCLUSION: The present investigation first introduced LAE as a candidate active ingredient for citrus preservative. A theoretical basis was provided for the development of preservation technology for high-value perishable fruit. According to the authors' knowledge this study is the first report on the inhibition mechanism of LAE in terms of oxidative stress.

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Antimicrobial effect of lauroyl arginate ethyl on Escherichia coli O157:H7 and Listeria monocytogenes on red oak leaf lettuce

Cited by 12 publications

References 40 publications

Adherence factors of enterohemorrhagic Escherichia coli O157:H7 strain Sakai influence its uptake into the roots of Valerianella locusta grown in soil

Adherence factors of enterohemorrhagic Escherichia coli O157:H7 strain Sakai influence its uptake into the roots of Valerianella locusta grown in soil

Nα-lauroyl-l-arginine ethyl ester monohydrochloride, an antimicrobial agent and its use: a review

Mechanism of fungal inhibition activity of Nα‐lauroyl‐L‐arginine ethyl ester (LAE) and potential in control of Penicillium expansum on postharvest citrus ‘Benimadonna’ (Citrus reticulata × Citrus sinensis)

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