Antimicrobial effect of Lagenaria siceraria (Mol.) Standley, against certain bacteria and fungal strains

Nagaraja, Y. P.; Geetha, K.; Vinay, Manon

doi:10.31018/jans.v3i1.169

Cited by 6 publications

(3 citation statements)

References 6 publications

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“…Moreover, the broth microdilution assay demonstrated general agreement with the disc diffusion results, which proved that chitosan nanoparticles loaded with Lagenaria siceraria or Thymus vulgaris are potent antibacterial agents against P. aeruginosa and VRSA strains with MIC values of 0.125-2 µg/mL and 1-8 µg/mL, respectively. Regarding the results obtained by Nagaraja and coauthors [55], L. siceraria had an antimicrobial activity against S. aureus with an MIC value of 0.25 × 10 3 mg/mL, which differs from Diniz et al [56], who found that thyme oil showed lower inhibitory activities against P. aeruginosa with MIC values ranging from 64 to 512 µg/mL. However, Keawchaoon and Yoksan [28] confirmed that chitosan nanoparticles with carvacrol exhibited antimicrobial activities against S. aureus compared to chitosan nanoparticles alone.…”

Section: Discussionmentioning

confidence: 88%

Chitosan-Loaded Lagenaria siceraria and Thymus vulgaris Potentiate Antibacterial, Antioxidant, and Immunomodulatory Activities against Extensive Drug-Resistant Pseudomonas aeruginosa and Vancomycin-Resistant Staphylococcus aureus: In Vitro and In Vivo Approaches

Taha,

Abd El-Aziz,

Abdelkhalek

et al. 2024

Antioxidants

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Antimicrobial resistance poses considerable issues for current clinical care, so the modified use of antimicrobial agents and public health initiatives, coupled with new antimicrobial approaches, may help to minimize the impact of multidrug-resistant (MDR) bacteria in the future. This study aimed to evaluate the antimicrobial, antioxidant, and immunomodulatory activities of Lagenaria siceraria, Thymus vulgaris, and their chitosan nanocomposites against extensive drug-resistant (XDR) Pseudomonas aeruginosa and vancomycin-resistant Staphylococcus aureus (VRSA) using both in vitro and in vivo assays. The in vitro antimicrobial susceptibilities of P. aeruginosa and VRSA strains revealed 100% sensitivity to imipenem (100%). All P. aeruginosa strains were resistant to cefoxitin, cefepime, trimethoprim + sulfamethoxazole, and fosfomycin. However, S. aureus strains showed a full resistance to cefoxitin, amoxicillin, ampicillin, erythromycin, chloramphenicol, and fosfomycin (100% each). Interestingly, all S. aureus strains were vancomycin-resistant (MIC = 32–512 μg/mL), and 90% of P. aeruginosa and S. aureus strains were XDR. The antimicrobial potential of Lagenaria siceraria and Thymus vulgaris nanocomposites with chitosan nanoparticles demonstrated marked inhibitory activities against XDR P. aeruginosa and VRSA strains with inhibition zones’ diameters up to 50 mm and MIC values ranging from 0.125 to 1 μg/mL and 1 to 8 μg/mL, respectively. The results of the in vivo approach in male Sprague Dawley rats revealed that infection with P. aeruginosa and S. aureus displayed significant changes in biochemical, hematological, and histopathological findings compared to the negative control group. These values returned to the normal range after treatment by chitosan nanoparticles, either loaded with Lagenaria siceraria or Thymus vulgaris. Real-time quantitative polymerase chain reaction (RT-qPCR) findings presented significant upregulation of the relative expression of the IL10 gene and downregulation of the IFNG gene throughout the experimental period, especially after treatment with chitosan nanoparticles loaded either with Lagenaria siceraria or Thymus vulgaris in comparison to the positive control groups. In conclusion, this is the first report suggesting the use of Lagenaria siceraria and Thymus vulgaris nanocomposites with chitosan nanoparticles as a promising contender for combating XDR P. aeruginosa and VRSA infections as well as a manager for inflammatory situations and oxidative stress-related disorders.

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Section: Discussionmentioning

confidence: 88%

Chitosan-Loaded Lagenaria siceraria and Thymus vulgaris Potentiate Antibacterial, Antioxidant, and Immunomodulatory Activities against Extensive Drug-Resistant Pseudomonas aeruginosa and Vancomycin-Resistant Staphylococcus aureus: In Vitro and In Vivo Approaches

Taha,

Abd El-Aziz,

Abdelkhalek

et al. 2024

Antioxidants

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“…Concerning antifungal activity, the degree of growth inhibition was notably higher for Aspergillus flavus and Aspergillus oryzae, while the inhibition of growth was comparatively lower in the case of Trichoderma harzianum. (Ameen et al, 2022;Nagaraja et al, 2011) showcasing their potential as innovative and effective agents in combating microbial infections.…”

Section: Antimicrobial Properties Of Cucurbitaceae Speciesmentioning

confidence: 99%

Scanning electron microscopic exploration of intricate pollen morphology and antimicrobial potentials of gourd family

Alhomaidi

2024

Microscopy Res & Technique

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This study employs scanning electron microscopy (SEM) to delve into the intricate pollen morphology of Cucurbitaceae (Gourd Family) species, unraveling the nuanced details of their structural features. Concurrently, the research investigates the antimicrobial potentials encoded within these pollen grains, shedding light on their possible applications in the realm of natural antimicrobial agents. Cucurbitaceae plants showcases significant antibacterial and antifungal potentials, underscoring its potential as a source for novel antimicrobial compounds. This research endeavors to provide a comprehensive analysis of pollen morphometry in 12 Cucurbitaceous species, with the primary goal of identifying pollen morpho‐types to enrich the taxonomic understanding of the Cucurbitaceae. Following the Erdtman protocol, the study involved processing, measuring, and capturing pollen grains using SEM techniques. Qualitative data were analyzed to evaluate the variations in pollen types, size, and exine stratified sculptured layering. The pollen grains exhibit characteristics of being monads, ranging in size from medium to very large, with the prevailing shape being prolate‐spheroidal in six species. Notably, the maximum polar axis diameter was recorded for Cucurbita pepo (106.3 μm), while the minimum was observed in Zehneria anomala (32.6 μm). The grains of Cucurbitaceae exhibit diverse surface patterns, including reticulate columellae, reticulate, verrucate‐scabrate, verrucate‐gemmate, echinate baculate, reticulate‐perforate, and micro‐reticulate. This SEM investigation illuminates the intricacies of Cucurbitaceae pollen morphology while concurrently highlighting their noteworthy antimicrobial potentials.Highlights High‐resolution imaging reveals complex pollen morphology. Identification of unique antimicrobial structures on Cucurbitaceae pollen (gourd family). Exploring medicinal potential: Antimicrobial properties of cucurbitaceae species.

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“…The fruit of L. siceraria is used, in traditional medicine, as remedy for different diseases, which include fever, jaundice, cough, pectoral, asthma and other bronchial disorders (Shah et al, 2010;Aslam and Najam, 2013;Prajapati et al, 2010). The leaf, fruit peel and seed extracts of the plant have been shown to possess antimicrobial activities against a number of microorganisms (Goji et al, 2006;Badmanaban and Patel, 2010;Rodge and Biradar, 2012;Nagaraja et al, 2011). In phytochemical studies, a variety of chemical constituents have been isolated, which include flavonoids, triterpenoids and glycosides (Kumar et al, 2012;Gangwal et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Study of bioactivities of lipid content of fresh Lagenaria siceraria seeds pulp and identification of its chemical constituents

Chaudhery¹,

Ahmed²,

Liaqat³

et al. 2014

J. Med. Plants Res.

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The aim of the present work was to analyse antimicrobial activities of the lipid content of seeds pulp of the fresh fruit of Lagenaria siceraria and identification of its chemical constituents by gas chromatography-mass spectrometry (GC-MS). Methanolic extract of the seeds pulp exhibited moderate to good efficacy against Achromobacter xylosoxidans, Pseudomonas aeruginosa, Bacillus megaterium and Escherichia coli having zone of inhibition (ZOI) 21.5, 20.0, 17.5 and 15.0 mm, respectively, at concentration 100 mg/ml. It showed better potency than antibiotic amoxylin against Enterobacter aerogenes, P. aeruginosa and Bacillus megaterium. The hexane fraction, that contained most of the lipid content, exhibited highest lethality against all the test microorganisms with ZOI 16.0 to 30.0 mm. It displayed highly significant efficacy against B. megaterium and Salmonella typhi with ZOI 30.0 and 22.5 mm, respectively. Minimum inhibitory concentration (MIC) of hexane fraction against B. megaterium and A. xylosoxidans was 0.40 mg/ml, while for others it ranged from 0.80 to 2.80 mg/ml. Palmitic acid exhibited significant potency against Staphylococcus aureus (ZOI, 20.0 mm, at concentration of 40 mg/ml), and moderately good efficacy against Stenotrophomonas maltophilia, Salmonella typhi and Enterobacter cloacae. Most compounds identified in the seeds pulp through GC-MS analysis were fatty acids or their esters. The compounds present in considerable concentration included azelaic acid (31.0%), (Z,Z)-9,12-octadecadienoic acid methyl ester (27.75%), and palmitic acid methyl ester (13.0%). Major component of ethyl acetate fraction was 4-hydroxybenzenemethanol (19.28%), while chloroform fraction contained 4-methoxymethylphenol (6.42%) and pentanoic acid 2-hydroxy-4-methyl-methyl ester (6.75%) as major constituents. The lipid content of seeds pulp of Lagenaria siceraria fruit contains components which are capable to inhibit bacterial growth, and the ones with strong anti-oxidative potential.

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Antimicrobial effect of Lagenaria siceraria (Mol.) Standley, against certain bacteria and fungal strains

Cited by 6 publications

References 6 publications

Chitosan-Loaded Lagenaria siceraria and Thymus vulgaris Potentiate Antibacterial, Antioxidant, and Immunomodulatory Activities against Extensive Drug-Resistant Pseudomonas aeruginosa and Vancomycin-Resistant Staphylococcus aureus: In Vitro and In Vivo Approaches

Chitosan-Loaded Lagenaria siceraria and Thymus vulgaris Potentiate Antibacterial, Antioxidant, and Immunomodulatory Activities against Extensive Drug-Resistant Pseudomonas aeruginosa and Vancomycin-Resistant Staphylococcus aureus: In Vitro and In Vivo Approaches

Scanning electron microscopic exploration of intricate pollen morphology and antimicrobial potentials of gourd family

Study of bioactivities of lipid content of fresh Lagenaria siceraria seeds pulp and identification of its chemical constituents

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