Antimicrobial Effect of 940 nm Diode Laser based on Photolysis of Hydrogen Peroxide in the Treatment of Periodontal Disease

Abstract

Moderate and severe periodontitis represents a challenge in the non-surgical periodontal therapy. Due to the lack of evidence regarding the antimicrobial effectiveness of 940 nm diode laser in periodontal treatment, this study aimed to evaluate the antimicrobial effect of hydrogen peroxide (H2O2) photolysis performed with 940 nm diode laser in the treatment of moderate and severe periodontitis. Twenty-five patients with 100 teeth were selected for this pilot study. The test teeth were randomly assigned to one of the four treatment groups: Group 1: scaling and root planning (SRP) (control group); and the following experimental groups: Group 2: H2O2; Group 3: 940 nm diode laser therapy; Group 4: 940 nm diode laser therapy and H2O2. Clinical examinations, like probing depth (PD), clinical attachment level (CAL) and bleeding on probing (BOP) were performed before and after the treatment. The microbiological evaluation, effectuated before and after the treatment, included nine periodontal bacteria species and investigated by means of real-time PCR assay. The clinical and bacterial differences in the tested groups, was assessed between control group and the other three experimental groups, as well as between the experimental groups. The total bacteria load was reduced for all four studied groups. Group 4 (diode laser + H2O2) showed significant bacterial reduction of the major periodontal bacteria like Pg., Tf., Td., Pi., Pm., Fn (p[0.001) than the other 3 groups (p]0.001). Also the periodontal clinical parameters, like PD, CAL and BOP showed a significant reduction after the photolysis of H2O2 with the 940 nm diode laser (p[0.001). Differences between tested groups showed a significant beneficial results in regard to Group 4.It is suggested that the photoactivation of H2O2 with the 940 nm diode laser can be used successfully in adjunctive to the non-surgical periodontal treatment as a bactericidal tool.

Effect of Hydrogen Peroxide Photoactivated Decontamination Using 940 nm Diode Laser in Periodontal Treatment: A Pilot Study

“…The first report about the photoactivation of 3% H 2 O 2 with the laser wavelength of 940 nm in the treatment of chronic periodontal disease was first introduced by Odor et al 14 Odor showed that the photoactivation of 3% H 2 O 2 with 940 nm diode laser without combining it with SRP, can efficiently eliminate ( p < 0.001) periodontal bacteria like P. gingivalis, T. denticola, T. forsythia, P. intermedia, P. micros, F. nucleatum, and E. nodatum and decrease the TBC. In addition, they compare the outcomes between each investigated group (SRP, H 2 O 2 alone, 940 nm laser alone, and photoactivation of H 2 O 2 with 940 nm) and the study showed great significant results ( p < 0.001), eliminating the major periodontal pathogenic species like P. gingivalis, T. denticola, T. forsythia, P. intermedia, P. micros and also for the TBC ( p < 0.005) when using the photoactivation of H 2 O 2 with 940 nm.…”

“…Although their trial study was investigating the periodontal clinical indexes for a short period of time (3 months), PPD and CAL showed great improvement without using the mandatory SRP with p < 0.005 and p < 0.008, respectively. 14 In a systematic review, Akram et al 31 evaluated the bactericidal outcomes of aPDT as an adjunct to SRP based on 17 in vivo studies. The wavelengths used by the investigated studies were ranging from 470 to 810 nm.…”

“…A more recent study investigated the use of H 2 O 2 photoactivation with 940 nm diode laser without the use of the SRP. 14 Based on these initial findings, we hypothesized that the photoactivation of H 2 O 2 with 940 nm diode laser in the presence of conventional SRP will improve the periodontal condition more than SRP alone and in combination with 940 nm diode laser. Therefore, the aim of this study was to evaluate the bactericidal effect and the clinical outcomes of the photoactivation of H 2 O 2 with 940 nm diode laser in combination with SRP in the nonsurgical periodontal treatment.…”

Clinical-Statistical Study on the Use of a Magnesium-Terpene-Based Substance in Polymeric Trays for Periodontal Treatment

“…If biofilm accumulation and growth is controlled the gingival tissues could heal and regain their initial characteristics, as the inflammatory changes in gingivitis are reversible [4]. However, this is not the case for the situation when the inflammatory reaction reaches the periodontal ligament or alveolar bone, where it inflicts permanent, irreversible damage [5][6][7][8][9]. If the etiological factor is not thoroughly controlled, the prognosis is unfavourable, as there is a high probability of incomplete inflammation remission or future disease relapse.…”

The Role of the Leucocytosis Parameter in the Occurrence of Pregnancy Complications in Women with Periodontal Disease

“…The periodontal disease is a multifactorial pathological disease initiated by a gram negative bacterial infection (Actinobacillus Actinomycetemcomitans, Porphyromonas gingivalis, Bacteroides forsythus, Porphyromonas gingivalis, Prevotella intermedia) that contribute to the generation of a local immune response, and the presence of single microorganisms is not the only factor responsible for periodontal destruction [1][2][3]. However, Porphyromonas gingivalis has been commonly associated with various systemic diseases [4].…”