Antimicrobial, cytotoxic effect and purification of bacteriocin from vancomycin susceptible Enterococcus faecalis and its safety evaluation for probiotization

Venkatesh, Perumal; Arul, Venkatesan

doi:10.1016/j.lwt.2016.12.048

Cited by 38 publications

(20 citation statements)

References 39 publications

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“…Bacteriocin KT11 was completely stable after other solvent treatments. Similar to our findings, it has been previously reported that enterocins remain completely stable after treatment with organic solvents ( Isleroglu et al, 2011 ; Perumal and Vankatesen, 2017 ). The stability of bacteriocins after treatment with solvents proves that bacteriocins do not have lipid moieties ( Yildirim et al, 2014 ).…”

Section: Resultssupporting

confidence: 93%

“…The CFS of E. faecalis KT11 showed antimicrobial activity against 4 of the 6 Gram-negative indicator bacteria, namely, P. aeruginosa, K. pneumoniae, S. marcescens and E. aerogenes , with inhibition zones ranging from 14 to 18 mm. Similar to our results, Perumal and Venkatasen (2017) obtained enterocin CV7 from E. faecalis CV7 and reported that this bacteriocin exhibited broad antimicrobial activity against both Gram-positive bacteria ( L. monocytogenes and S. aureus ) and Gram-negative bacteria ( Salmonella sp., S. thypi , S. enterica , E. coli and Vibrio fischeri). Abriouel et al (2005) reported that two enterocins obtained from E. faecalis inhibited E. coli and Shigella sonnei .…”

Section: Resultssupporting

confidence: 90%

“…2 ). Similarly, it has been reported that bacteriocins of E. faecium LR/6 ( Kumar et al, 2010 ), E. faecalis CV7 ( Perumal and Vankatesen, 2017 ), and E. hirae LD3 ( Gupta et al., 2016 ) were stable in the pH ranges 2–6, 4–6 and 2–6, respectively. Du et al (2017) reported that the anti-listerial activity of a bacteriocin obtained from E. durans 152 was completely retained in the pH range of 2–8.…”

Section: Resultssupporting

confidence: 56%

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Antimicrobial Activity of a Bacteriocin Produced by Enterococcus faecalis KT11 against Some Pathogens and Antibiotic-Resistant Bacteria

Abanoz

Kunduhoğlu

2018

Korean J Food Sci Anim Resour

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In this study, the antimicrobial activity of a bacteriocin produced by Enterococcus faecalis KT11, isolated from traditional Kargı Tulum cheese, was determined, and bacteriocin KT11 was partially characterized. The results showed that bacteriocin KT11 was antagonistically effective against various Gram-positive and Gram-negative test bacteria, including vancomycin- and/or methicillin-resistant bacteria. The activity of bacteriocin KT11 was completely abolished after treatment with proteolytic enzymes (proteinase K, α-chymotrypsin, protease and trypsin), which demonstrates the proteinaceous nature of this bacteriocin. Additionally, bacteriocin KT11 remained stable at pH values ranging from 2 to 11 and after autoclaving at 121℃ for 30 min. In addition, the activity of bacteriocin KT11 was stable after treatment with several surfactants (EDTA, SDS, Triton X-100, Tween 80 and urea) and organic solvents (chloroform, propanol, methanol, ethyl alcohol, acetone, hexane and ethyl ether). Cell-free supernatant of E. faecalis KT11 was subjected to ammonium sulfate precipitation and then desalted by using a 3.5-kDa cut-off dialysis membrane. The bacteriocin activity was determined to be 711 AU/mL in the dialysate. After tricine-SDS-PAGE analysis, one peptide band, which had a molecular weight of ~3.5 kDa, exhibited antimicrobial activity. Because the bacteriocin KT11, isolated from E. faecalis KT11, exhibits a broad antimicrobial spectrum, heat stability and stability over a wide pH range, this bacteriocin can be used as a potential bio-preservative in foods. Additionally, bacteriocin KT11 alone or in combination with conventional antibiotics may provide a therapeutic option for the treatment of multidrug-resistant clinical pathogens after further in vivo studies.

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Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 90%

Section: Resultssupporting

confidence: 56%

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Antimicrobial Activity of a Bacteriocin Produced by Enterococcus faecalis KT11 against Some Pathogens and Antibiotic-Resistant Bacteria

Abanoz

Kunduhoğlu

2018

Korean J Food Sci Anim Resour

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“…The most potent strains were selected for molecular identification such as DV1-1, PKS2-1, and MP6/2, representing each clonal group, and found that DV1-1 and PKS2-1 were E. faecium and MP6/2 was P. pentosaceus. Several studies have been reported the BLOS producing E. faecium and P. pentosaceus (Ahmadova et al, 2013;Perumal & Venkatesan, 2017;Todorov & Dicks, 2009;Toit et al, 2000;Tulini et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Ot has been proved that the bacteriocin of E. faecalis CV7 has anti-cell proliferative property on HeLa cells. E. faecalis CV7 could be a probiont without any harmful genetic background (Perumal & Venkatesan, 2017). Another broad-spectrum bacteriocin producing E. faecium GM-1, isolated from infant feces, was described with temperature, and pH resistance, similar to enterocin P (Kang & Lee, 2005).…”

Section: Discussionmentioning

confidence: 99%

Screening and identification of bacteriocin-like inhibitory substances producing lactic acid bacteria from fermented products

et al. 2020

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Bacteriocins: Properties and potential use as antimicrobials

Darbandi

Asadi

Ari

et al. 2021

Clinical Laboratory Analysis

162

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A variety of bacteriocins originate from lactic acid bacteria, which have recently been modified by scientists. Many strains of lactic acid bacteria related to food groups could produce bacteriocins or antibacterial proteins highly effective against foodborne pathogens such as Staphylococcus aureus, Pseudomonas fluorescens, P. aeruginosa, Salmonella typhi, Shigella flexneri, Listeria monocytogenes, Escherichia coli O157:H7, and Clostridium botulinum. A wide range of bacteria belonging primarily to the genera Bifidobacterium and Lactobacillus have been characterized with different health‐promoting attributes. Extensive studies and in‐depth understanding of these antimicrobials mechanisms of action could enable scientists to determine their production in specific probiotic lactic acid bacteria, as they are potentially crucial for the final preservation of functional foods or for medicinal applications. In this review study, the structure, classification, mode of operation, safety, and antibacterial properties of bacteriocins as well as their effect on foodborne pathogens and antibiotic‐resistant bacteria were extensively studied.

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Antimicrobial, cytotoxic effect and purification of bacteriocin from vancomycin susceptible Enterococcus faecalis and its safety evaluation for probiotization

Cited by 38 publications

References 39 publications

Antimicrobial Activity of a Bacteriocin Produced by Enterococcus faecalis KT11 against Some Pathogens and Antibiotic-Resistant Bacteria

Antimicrobial Activity of a Bacteriocin Produced by Enterococcus faecalis KT11 against Some Pathogens and Antibiotic-Resistant Bacteria

Screening and identification of bacteriocin-like inhibitory substances producing lactic acid bacteria from fermented products

Bacteriocins: Properties and potential use as antimicrobials

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