Antimicrobial Compounds from the Australian Desert Plant <i>Eremophila neglecta</i>

Ndi, Chi P.; Semple, Susan J.; Griesser, Hans J.; Pyke, Simon M.; Barton, Mary

doi:10.1021/np070180r

Cited by 50 publications

(71 citation statements)

References 16 publications

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“…EN4 was extracted from freshly collected E. neglecta plant material with a purity of Ն95% (as determined by nuclear magnetic resonance analysis) as described previously (12) and stored at Ϫ20°C. EN4 was dissolved in 1% dimethyl sulfoxide (DMSO) (Merck, Darmstadt, Germany) in phosphate-buffered saline (PBS) (Reagens, Basel, Switzerland) (DMSO-PBS) up to a concentration of 400 g/ml.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial Properties of 8-Hydroxyserrulat-14-en-19-oic Acid for Treatment of Implant-Associated Infections

Nowakowska

Griesser

Textor

et al. 2013

Antimicrob Agents Chemother

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Treatment options are limited for implant-associated infections (IAI) that are mainly caused by biofilm-forming staphylococci. We report here on the activity of the serrulatane compound 8-hydroxyserrulat-14-en-19-oic acid (EN4), a diterpene isolated from the Australian plant Eremophila neglecta. EN4 elicited antimicrobial activity toward various Gram-positive bacteria but not to Gram-negative bacteria. It showed a similar bactericidal effect against logarithmic-phase, stationary-phase, and adherent Staphylococcus epidermidis, as well as against methicillin-susceptible and methicillin-resistant S. aureus with MICs of 25 to 50 g/ml and MBCs of 50 to 100 g/ml. The bactericidal activity of EN4 was similar against S. epidermidis and its ⌬ica mutant, which is unable to produce polysaccharide intercellular adhesin-mediated biofilm. In time-kill studies, EN4 exhibited a rapid and concentration-dependent killing of staphylococci, reducing bacterial counts by >3 log 10 CFU/ml within 5 min at concentrations of >50 g/ml. Investigation of the mode of action of EN4 revealed membranolytic properties and a general inhibition of macromolecular biosynthesis, suggesting a multitarget activity. In vitro-tested cytotoxicity on eukaryotic cells was time and concentration dependent in the range of the MBCs. EN4 was then tested in a mouse tissue cage model, where it showed neither bactericidal nor cytotoxic effects, indicating an inhibition of its activity. Inhibition assays revealed that this was caused by interactions with albumin. Overall, these findings suggest that, upon structural changes, EN4 might be a promising pharmacophore for the development of new antimicrobials to treat IAI.

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Section: Methodsmentioning

confidence: 99%

Antimicrobial Properties of 8-Hydroxyserrulat-14-en-19-oic Acid for Treatment of Implant-Associated Infections

Nowakowska

Griesser

Textor

et al. 2013

Antimicrob Agents Chemother

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“…Cytotoxic effect on P388D1 mouse lymphoblast cells [17] E. neglecta Ether extract (a) 8,19-dihydroxyserrulat-14-ene (b) 8-hydroxyserrulat-14-en-19-oic acid (c) Biflorin Antimicrobial activity against Staphylococcus aureus Antimicrobial activity against Gram-positive bacteria S. aureus, Streptococcus pyogenes, and S Streptococcus pneumoniae. [39] E. serrulata Ether extract (a) 9-methyl-3-(4-methyl-3-pentenyl)-2,3dihydronaphtho[1,8-bc]pyran-7,8-dione (b) 20-acetoxy-8-hydroxyserrulat-14-en-19-oic acid. (c) 8,20-dihydroxyserrulat-14-en-19-oic acid (d) 8,20diacetoxyserrulat-14-en-19-oic acid Antimicrobial activity against S. aureus [40] E. sturtii Ethanol crude extract 3,8-dihydroxyserrulatic acid Serrulatic acid…”

Section: E Maculatamentioning

confidence: 99%

“…Two of these compounds showed antimicrobial activity against Gram-positive bacteria including S. aureus, S. pyogenes, and S. pneumoniae. [39] The bioactivity spectrum of these compounds was recently examined across a wider range of Gram-positive and Gram-negative bacteria including human and veterinary pathogens, and some multidrug-resistant isolates to explore the range of potential utility of these novel antibacterials. Both 8,19dihydroxyserrulat-14-ene (103) and 8-hydroxyserrulat-14en-19-oic acid (105) exhibited antibacterial activity against all Gram-positive bacteria tested.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

The genus Eremophila (Scrophulariaceae): an ethnobotanical, biological and phytochemical review

Singab

Youssef

Ashour

2013

Journal of Pharmacy and Pharmacology

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Objectives Eremophila (Scrophulariaceae) is an endemic Australian genus with 214 species, which is commonly known as Fuchsia bush, Emu bush or Poverty bush. Plants of this genus played an important role for the Australian Aborigines who used them widely for medicinal and ceremonial purposes. Many studies have been carried out on many species of this genus and have generated immense data about the chemical composition and corresponding biological activity of extracts and isolated secondary metabolites. Key findings Thorough phytochemical investigations of different Eremophila species have resulted in the isolation of more than 200 secondary metabolites of different classes with diterpenes as major constituents. Biological studies and traditional clinical practice demonstrated that Eremophila and its bioactive compounds possess various pharmacological properties. Plants were employed especially as a cardiotonic drug and also as potent anti-inflammatory, antimicrobial and antiviral agents. Summary Further investigations are required to explore other Eremophila species, to evaluate the different biological activities of either their extracts or the isolated compounds and the possible underlying modes of action.

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“…Many of them are already well established in the food industry to prevent pathogenic contamination (112,116). An extract from the Australian desert plant Eremophila neglecta has been shown to possess antibacterial activity in a survey of candidate plants that may bear novel antimicrobial compounds (139). An extract from the Australian desert plant Eremophila neglecta has been shown to possess antibacterial activity in a survey of candidate plants that may bear novel antimicrobial compounds (139).…”

Section: Essential Oilsmentioning

confidence: 99%