Antimicrobial Behavior of Polyelectrolyte Multilayer Films Containing Cetrimide and Silver

Grunlan, Jaime C.; Choi, John K.; Lin, Andy

doi:10.1021/bm049528c

Cited by 139 publications

(146 citation statements)

References 43 publications

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“…Grunlan and coworkers used this method for polyelectrolyte multilayers that contains silver nitrate and/or quaternary ammonium salts [230]. In this test, agar plates were uniformly inoculated with solution of S. aureus or E. coli containing approximately 5 ×10 6 CFU/mL.…”

Section: Methods For Evaluating Efficiency Of Biocidal Surfacesmentioning

confidence: 99%

Infectious disease: Connecting innate immunity to biocidal polymers

Gabriel

Som

Madkour

et al. 2007

Materials Science and Engineering: R: Reports

253

258

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Infectious disease is a critically important global healthcare issue. In the U.S. alone there are 2 million new cases of hospital-acquired infections annually leading to 90,000 deaths and 5 billion dollars of added healthcare costs. Couple these numbers with the appearance of new antibiotic resistant bacterial strains and the increasing occurrences of community-type outbreaks, and clearly this is an important problem. Our review attempts to bridge the research areas of natural host defense peptides (HDPs), a component of the innate immune system, and biocidal cationic polymers. Recently discovered peptidomimetics and other synthetic mimics of HDPs, that can be short oligomers as well as polymeric macromolecules, provide a unique link between these two areas. An emerging class of these mimics are the facially amphiphilic polymers that aim to emulate the physicochemical properties of HDPs but take advantage of the synthetic ease of polymers. These mimics have been designed with antimicrobial activity and, importantly, selectivity that rivals natural HDPs. In addition to providing some perspective on HDPs, selective mimics, and biocidal polymers, focus is given to the arsenal of biophysical techniques available to study their mode of action and interactions with phospholipid membranes. The issue of lipid type is highlighted and the important role of negative curvature lipids is illustrated. Finally, materials applications (for instance, in the development of permanently antibacterial surfaces) are discussed as this is an important part of controlling the spread of infectious disease.

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Section: Methods For Evaluating Efficiency Of Biocidal Surfacesmentioning

confidence: 99%

Infectious disease: Connecting innate immunity to biocidal polymers

Gabriel

Som

Madkour

et al. 2007

Materials Science and Engineering: R: Reports

253

258

View full text Add to dashboard Cite

show abstract

“…Measurements were recorded continuously at the fundamental frequency of 5 MHz, as well as the third, fifth, and seventh overtones (15,25, and 35 MHz, respectively). The desired solutions were allowed to flow through the chamber as required.…”

Section: Quartz Crystal Microbalance Analysismentioning

confidence: 99%

“…10 The architecture of the film from the LbL electrostatic deposition can be customized by tuning several operating parameters such as polyelectrolyte chemical nature, pH and ionic strength, number of layers deposited, dipping and rinsing durations, and film posttreatment. [11][12][13] As such, LbL assembled films have found applications in the biomedical field including the construction of biocompatible device surfaces, 14 antibacterial coatings, 15 biosensors, 16 and biomimetic three-dimensional structures. 17 Since the multilayers are built up on the basis of electrostatic interaction between the oppositely charged chains of polycations and polyanions, exposure to solvents, pH and ionic strength jumps can have an influence on their structural stability.…”

Section: Introductionmentioning

confidence: 99%

Structural stability and bioapplicability assessment of hyaluronic acid–chitosan polyelectrolyte multilayers on titanium substrates

Chua

Neoh

Shi

et al. 2008

J Biomedical Materials Res

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Since bacterial infections associated with implants remain a major cause of their failure, this study investigated the use of polyelectrolyte multilayers (PEMs) comprising hyaluronic acid (HA) and chitosan (CH) to confer antibacterial properties on titanium (Ti). HA and CH were deposited on Ti using the layer-by-layer deposition method. The antibacterial efficacy of the functionalized Ti substrates was assessed using Escherichia coli and Staphylococcus aureus. The number of adherent bacteria on Ti functionalized with HA and CH PEMs was up to an order of magnitude lower than that on the pristine Ti. The effects of chemical crosslinking of the PEMs on the structural stability and antibacterial efficacy were investigated. The chemical crosslinking of the PEMs imparts greater structural stability and preserves the antibacterial properties even after the prolonged immersion in phosphate-buffered saline. The cytotoxicity of the PEMs to osteoblasts was evaluated using the MTT assay. The results showed that the biocompatible and long-lasting antibacterial nature of the functionalized Ti substrates offers great potential for reducing implant-associated infections.

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“…Antibacterial films incorporating silver particles [30][31][32][33][34][35] or biocidal polycations [36] have been successfully prepared by this technique. Recently, polyelectrolyte multilayers were also functionalized with antifungal [37] and antimicrobial peptides.…”

Section: Full Papermentioning

confidence: 99%

Incorporation of a Hydrophobic Antibacterial Peptide into Amphiphilic Polyelectrolyte Multilayers: A Bioinspired Approach to Prepare Biocidal Thin Coatings

Guyomard

Dé

Jouenne

et al. 2008

Adv Funct Materials

118

109

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A non‐water‐soluble natural antibacterial peptide, gramicidin A, has been successfully incorporated into polyelectrolyte assemblies to elaborate biocidal thin films. For this, we used a double strategy, the first step of which consists of complexing the peptide by a non‐denaturing anionic amphiphilic polysaccharide, namely a hydrophobically modified carboxymethylpullulan. We demonstrate that the use of this amphiphilic anionic derivative allows to efficiently solubilize the peptide in aqueous solution, without denaturation. The amount of peptide solubilized by the amphiphilic polysaccharide was optimized by systematically varying the hydrophobicity and the molar mass of the CMP derivative. In a second step, the negatively charged complex was layer‐by‐layer assembled with cationic poly(L‐lysine) to form biofunctionalized thin films. The amount of peptide incorporated in the multilayers was controlled by changing the number of deposited complex layers, and was quantified by UV spectroscopy. The antibacterial activity of the resulting biofunctionalized films was evidenced against a gram‐positive bacterium, E. faecalis. We demonstrated that the biocidal activity resulted from a double mechanism: contact between bacteria and the film surface, and release of the peptide into the solution surrounding the film. We also showed that the peptide was not completely removed from the film after rinsing, which insured preservation of the biocidal activity of the film surface.

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Antimicrobial Behavior of Polyelectrolyte Multilayer Films Containing Cetrimide and Silver

Cited by 139 publications

References 43 publications

Infectious disease: Connecting innate immunity to biocidal polymers

Infectious disease: Connecting innate immunity to biocidal polymers

Structural stability and bioapplicability assessment of hyaluronic acid–chitosan polyelectrolyte multilayers on titanium substrates

Incorporation of a Hydrophobic Antibacterial Peptide into Amphiphilic Polyelectrolyte Multilayers: A Bioinspired Approach to Prepare Biocidal Thin Coatings

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