Antimicrobial, antioxidant, and cytotoxic activities of endhopitic fungi Chaetomium sp. isolated from Phyllanthus niruri Linn: in vitro and in silico studies

Abstract

Endophytic fungi Chaetomium sp isolated from Phyllanthus niruri Linn. Mycelium powder was extracted by using ethyl acetate. Extract was fractionated using n-hexane, dichloromethane and ethanol 96%. The antimicrobial test was carried out using disc diffusion and microdilution methods. The antioxidant activity of the fraction was determined using hydrogen peroxide free radical scavenging and reducing power capacity activities. The cytotoxicity assay of the fraction against T47D breast cancer cell was carried out using dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide method (MTT). The in silico prediction of chemical substances which are reported exist in Chaetomium sp. performed using AutoDockVina embedded in PyRx version 8.0. Dichloromethane fraction was found as the most active sample against Escherichia coli (IC 50 20.76 g/mL), Staphylococcus aureus (IC 50 70.15 g/mL), Salmonella typhi (49.13 g/mL) and was found as the most high phenolic content with value 47.44 mg GAE/g fraction, whereas the best antioxidant activity was performed by ethanol 96% fraction (85%). Cytotoxicity assay against T47D cell line showed dichloromethane fraction have highest activity with IC 50 10.76 g/mL. The docking studies showed that compounds bearing xanthone structure were potential for maltose binding periplasmic and human aromatase associating with their potencies as antibacteria and anticancer. Endophytic fungi Chaetomium sp. was isolated from Phyllanthus niruri using n-hexane, dichloromethane and ethanol fractions was studied its various biological activities as antimicrobial, antioxidant and cytotoxic agent against breast cancer cell.

Bioprospecting of endophytes in medicinal plants of Thar Desert: An attractive resource for biopharmaceuticals

“… New taxa related to Streptomyces Fusarium sp., Chaetomium sp., and Colletotrichum sp. 1,8-Dihydroxy-3-methoxy-6-methylanthracene-9,10-dione, 1,3,8-Trihydroxy-6-methoxyanthracene-9,10-dione, and Dichloromethane Antimicrobial and antioxidant activities Khan et al [ 197 ]; Rollando et al [ 139 ]; Yuniati and Rollando [ 207 ]; Bhatia et al [ 140 ]; Hannana et al [ 141 ]; Ezzat et al [ 185 ] Mucuna pruriens (L.) DC. Roots, stems, seeds and flowers The seeds are ground with almond, and eaten in case of sexual debility, rheumatism and as tonic.…”

In Silico Study of Histo-aspartic Protease (HAP) Inhibitor from Indonesian Medicinal Plants: Anti-malarial Discovery

“…In this study, a total of 238 compounds derived from 43 Indonesian medicinal plants uses in malarial therapy were collected. The mechanism of action of these compounds has not been investigated with certainty, and scientific approaches are needed to find out the mechanism of action (Rollando et al, 2017). Histo-aspartic protease (HAP) is one of the certain enzymes in complex with the crystal structure of inhibitor KNI-10006 found in P. falciparum which causes malaria (Bhaumik et al, 2009).…”