Antimicrobial, anti-inflammatory and antioxidant activities of natural organic matter extracted from cretaceous shales in district Nowshera-Pakistan

Khuda, Fazli; Anjum, M.S. Rashida; Khan, Suleman; Khan, Hamayun; Sahibzada, Muhammad Umar Khayam; Khusro, Ameer; Jan, Asif; Ullah, Naveed; Shah, Yatrik M.; Zakiullah, *; Abbas, Muhammad; Iftikhar, Tayyaba; Idris, Abubakr M.; Khandaker, Mayeen Uddin; Emran, Talha Bin

doi:10.1016/j.arabjc.2021.103633

Cited by 14 publications

(7 citation statements)

References 33 publications

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“…Recently, it was found by [ 39 ] that FA extract has a similar DPPH radical scavenging ability to ascorbic acid, and reached the inhibition of ~50% at a concentration of 1000 µg/mL. In the case of natural organic matter (FA and HA fractions) extracted from cretaceous shales, an antioxidant efficiency similar to that of ascorbic acid was also observed in the concentration range of 25–1000 µg/mL [ 40 ]. However, ~50% inhibition of DPPH radical decomposition was already achieved at a much lower concentration (~250 µg/mL) than above.…”

Section: Resultsmentioning

confidence: 99%

Screening of Humic Substances Extracted from Leonardite for Free Radical Scavenging Activity Using DPPH Method

Csicsor

Tombácz

2022

Molecules

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Humic substances (HSs) have been researched for a long time and still manage to surprise humanity today. According to the latest research, in addition to their previously well-known effects, they also have antioxidant properties. However, this previous research does not examine the difference in the antioxidant effect of the fractions extracted/produced in different processes; they do not consider the light absorption of the HSs, which falsifies analysis based on the measurement of color change over time. In the present work, HS fractions were obtained from leonardite, the extraction processes can also be implemented on an industrial scale. The fractions were characterized by elementary analysis, UV–Vis and FT-IR spectroscopies, to prove that our self-extracted samples have similar characteristics to the International Humic Substances Society (IHSS) standard samples. The different methods of HS fractionation affected the elemental composition, and the spectral characteristics. The antioxidant effect was investigated using the DPPH method to screen the antioxidant efficiency of humic, fulvic, and himatomelanic acids. In addition, we compared our results with the IHSS standard samples to obtain a more comprehensive picture of the antioxidant effect of HSs extracted in different ways according to the DPPH method. Based on our results, the extraction method affects not only the physico-chemical properties but also the free radical scavenging activity of the fractions.

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Section: Resultsmentioning

confidence: 99%

Screening of Humic Substances Extracted from Leonardite for Free Radical Scavenging Activity Using DPPH Method

Csicsor

Tombácz

2022

Molecules

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“…Other anti-inflammatory effects were attributed to the ability to decrease in pro-inflammatory cytokines and acute phase protein after HAs' preparation application [78][79][80]. Khuda et al [81] found the HS anti-inflammatory effect is boosted by cyclooxygenase and lipoxygenase inhibition in a dose-dependent manner, and possibly other vasoactive compounds, like serotonin and histamine inhibition. HS and its different fractions have shown efficiency in wide spectrum antiviral and immunostimulating activities against HIV, H1N1 influenza, herpes simplex virus-1, Coxsackie virus A9, or tick-borne encephalitis virus [82][83][84][85][86][87][88][89][90].…”

Section: In Vitro and In Vivo Efficacy Of Humic Acidsmentioning

confidence: 99%

Therapeutic Efficiency of Humic Acids in Intoxications

Vašková

Stupák

Uğurbaş

et al. 2023

Life

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Humins, humic and fulvic acids represent molecules with complex structures. These compounds comprising humic substances (HS) exist naturally in soil, brown coal, peat, and water. They are formed during the decomposition and transformation of organic matter (animal and plant remains) and their formation explains several theories. Within their chemical structures, there are numerous phenolic and carboxyl groups and their derivatives that affect their different properties, such as their solubility in water or their absorption of cations or mycotoxins. The manifold chemical structure of HS alters their polyelectrolyte character and thus their chelating efficiency. For many years, HS have been studied due to their detoxification, anti-, and pro-inflammatory or anticancer and antiviral ability. This article summarizes the antioxidant and adsorption properties of humic acids, highlighting their usefulness in intoxications.

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“…The condition results in notable impairment and subsequent decline in the overall well-being, resulting in a considerable socio-economic burden (Buch and Emery 2002). Steroidal and non-steroidal anti-inflammatory drugs (NSAIDs) are the current therapeutic approaches for managing inflammatory illnesses (Langman 1998;Khuda et al 2022). The prolonged use of these medications results in detrimental side effects and compromises the integrity of the human biological system, including the liver, gastrointestinal tract, and other relevant systems.…”

Section: Introductionmentioning

confidence: 99%

In vitro assessment on anti-inflammatory and anti-lipidemic properties of selected plant species

Ezung,

Kalivarathan,

Khusro

et al. 2024

BioRes

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Preliminary assessment for anti-inflammatory and anti-lipidemic properties was done with different solvent extracts derived from Urtica urens and Polygonum chinense leaves through in vitro experimentation. To evaluate anti-inflammatory properties, the stability of human red blood cells membranes and the denaturation activity of proteins were assessed. For anti-lipidemic effects, an assay was conducted to measure the inhibition of HMG-CoA reductase. The results of membrane stabilization showed IC50 values of 480.96 ± 0.02 and 319.41 ± 0.19 µg/mL for ethyl acetate extract of U. urens and P. chinense, respectively. The standard drug Diclofenac sodium exhibited IC50 value of 240.37 ± 0.04 µg/mL. For protein denaturation, IC50 values were determined as 221.75 ± 0.2 and 315.76 ± 0.19 µg/mL for U. urens and P. chinense, respectively. The IC50 value of the standard drug was calculated as 126.7 ± 0.34. The IC50 values towards HMG-CoA reductase inhibition were subsequently determined as 29.84 ± 0.35 µg/mL for U. urens and 24.34 ± 0.04 µg/mL for P. chinense against the standard drug Diclofenac sodium (7.52 ± 0.43 µg/mL). GC-MS chromatograms revealed the presence of bioactive compounds in ethyl acetate extract of P. chinense leaves. This work is substantiation for the traditional therapeutic utilization of these extracts.

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Antimicrobial, anti-inflammatory and antioxidant activities of natural organic matter extracted from cretaceous shales in district Nowshera-Pakistan

Cited by 14 publications

References 33 publications

Screening of Humic Substances Extracted from Leonardite for Free Radical Scavenging Activity Using DPPH Method

Screening of Humic Substances Extracted from Leonardite for Free Radical Scavenging Activity Using DPPH Method

Therapeutic Efficiency of Humic Acids in Intoxications

In vitro assessment on anti-inflammatory and anti-lipidemic properties of selected plant species

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