Antimicrobial Annona muricata L. (soursop) extract targets the cell membranes of Gram-positive and Gram-negative bacteria

Pinto, Nícolas de Castro Campos; Campos, Lara Melo; Evangelista, Anna Carolina S.; Lemos, Ari S.O.; Silva, Thiago Pereira da; Melo, Rossana C. N.; Lourenço, Caroline Caramano de; Salvador, Marcos José; Apolônio, Ana Carolina Morais; Scio, Elita; Fabri, Rodrigo Luiz

doi:10.1016/j.indcrop.2017.05.054

Cited by 45 publications

(37 citation statements)

References 39 publications

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“…Recently, soursop fruit ( Annona muricata L.) has attained considerable interest from the researchers because it has high nutritional value especially in carbohydrates and minerals, and also served as therapeutic agents (Agu & Okolie, ; Pinto et al, ). However, soursop fruit has an apparent attribute, which it will turn soft and get a dent in the flesh easily, and releases a displeasing strong odor when ripe (Espinosa, Ortiz, Tovar, Mata, & Montalvo, ), makes it tends to be rejected for further processing.…”

Section: Introductionmentioning

confidence: 99%

Moisture sorption isotherm and shelf‐life prediction of anticaking agent incorporated spray‐dried soursop (Annona muricata L.) powder

Chang

Karim

Mohammed³

et al. 2019

J Food Process Engineering

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The moisture sorption isotherm (MSI) and shelf lives of soursop powders that had been incorporated with two commercial anticaking agents, calcium silicate (CS) and tricalcium phosphate (TCP), and inserted into aluminum laminated polyethylene pouches under room (RT, 25 ± 1 C) and elevated (ET, 38 ± 1 C) temperature, maintained at 93 ± 1% RH, were investigated. The powder possessed a Type III Brunauer's classification isotherm curve. The monolayer moisture content (M o ) was 0.023 ± 0.001 g H 2 O/g ds. A combination of low mean relative percent deviation modulus, E (2.18-3.75%), small sum of square residual, SSR (1.03 × 10 −5 -8.51 × 10 −6 ), and high coefficient of determination (R 2 > .99) indicating the moisture sorption data was sufficiently good fit using GAB model. Powder without anticaking agent stored at ET has the shortest estimated shelf life (~86 days) while 1.0 g/100 g TCP stored at RT exhibited the longest shelf life (~316 days). This study demonstrated that the incorporation of anticaking agents is effective to maintain the quality of powder by extending the shelf life by lowering moisture adsorption. The acceptability of reconstituted soursop powder based on hedonic test showed the scores for all attributes were higher than six.

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Section: Introductionmentioning

confidence: 99%

Moisture sorption isotherm and shelf‐life prediction of anticaking agent incorporated spray‐dried soursop (Annona muricata L.) powder

Chang

Karim

Mohammed³

et al. 2019

J Food Process Engineering

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“…In this research, Pseudoalteromonas, a Gram-negative bacteria have the capability of producing substances in a single fermentation because some of the substances are bioactive compounds (Isnansetyo & Kamei, 2009). Pseudoalteromonas rubra was able to prevent the loss of intracellular proteins, and reduced the access of hydrolytic enzymes and some compounds produced by S. aureus (Pinto et al, 2017). Extract from Pseudoalteromonas rubra KJB-07 had stronger activity than from V. salarius NP31-01.…”

Section: Discussionmentioning

confidence: 92%

Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia

Kristiana

Bedoux

Pals

et al. 2020

PeerJ

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The aims of this work are to isolate bacterial symbionts from nudibranchs and subsequently to determine anti-Methicillin resistant Staphylococcus aureus (MRSA), cytotoxicity and anti-Herpes simplex virus type 1 (HSV-1) activities of bio compounds. A total of 15 species of nudibranchs were collected from Karimunjawa and five species from Bali, respectively. A total of 245 bacteria isolates were obtained. The anti-MRSA activity screening activity indicated two active bacteria. Ethyl acetate extracts from supernatants, indicating extracelullar compounds, showed an inhibition zone against MRSA at concentrations of 500-1,000 µg/ml. DNA sequence analysis showed that the strain KJB-07 from Phyllidia coelestis was closely related to Pseudoalteromonas rubra, whereas the strain NP31-01 isolated from Phyllidia varicosa was closely related to Virgibacillus salarius. The extract of Pseudoalteromonas rubra was cytotoxic to Vero cells at a concentration of 75 µg/ml. The extract of V. salarius presented no cytotoxicity at concentrations of 5-1,000 µg/ml. No anti HSV-1 was observed for both isolated bacteria. This is the first study describing research on anti-MRSA, cytotoxicity and anti HSV-1 activity of bacterial symbionts from the viscera of nudibranch. Compounds produced by Pseudoalteromonas rubra and V. salarius, had potential anti-MRSA activity. However, only extracts from Pseudoalteromonas rubra showed cytotoxic effects on Vero cells. Three compounds were identified by LC/MS after purification from culture supernatant. activity of marine bacteria associated with sponges from the waters off the coast of South East India. Microbiological Research 161 (3):252-262 DOI 10.1016/j.micres.2005.09.002. Avila C. 2006. Molluscan natural products as biological models: chemical ecology, histology, and laboratory culture. Chand S, Karuso P. 2017. Isolation and total synthesis of two novel metabolites from the fissurellid mollusc Scutus antipodes. Tetrahedron Letters 58(10):P. 1992. A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification. Research in Microbiology 143(8):785-790

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“…Saline suspensions of freshly grown bacterial strains (10 8 CFU mL -1 ) were inoculated with different concentrations of psychorubrin (MIC, 0.5MIC, and 0.25MIC values) supplemented with MHB. Optical density (OD) at 600 nm was recorded in a spectrophotometer (Multiskan Go, Thermo Scientific, Waltham, MA, United States) at 2, 4, 6, 8, and 24 h of bacterial growth at 37°C as before ( Pinto et al, 2017a , b ). Graphs of turbidity versus incubation time were plotted.…”

Section: Methodsmentioning

confidence: 99%

“…Because antimicrobial resistance and biofilm formation are a global public health challenge, there is a striking need of development of new biologically active molecules against multidrug-resistant bacteria and the adoption of medicinal crude extracts of plants to treat infectious diseases ( Subramani et al, 2017 ). In this context, our group has been studying different potential bioactive antibacterial compounds ( Pinto et al, 2017a , b ), including a natural product termed psychorubrin, a napthoquinone commonly found in a variety of plants ( Fabri et al, 2009 , 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and Antibiofilm Activities of Psychorubrin, a Pyranonaphthoquinone Isolated From Mitracarpus frigidus (Rubiaceae)

et al. 2018

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Psychorubrin, a natural pyranonaphthoquinone found in different plants, has become an interesting compound in the search for new antimicrobial therapeutic agents. Here, we investigated the potential antagonistic activity of psychorubrin against planktonic and biofilm bacteria. First, psychorubrin was tested against several Gram-positive and Gram-negative bacteria strains by a broth microdilution susceptibility method. Second, bacterial killing assay, bacterial abundance, and membrane viability were evaluated. The nucleotide leakage assay was used to verify membrane destabilization while antibiofilm activities were analyzed by the effect on established biofilm, static biofilm formation, isolation of biofilm matrix assay and scanning electron microscopy. In parallel, the combinatorial effect of psychorubrin and chloramphenicol was evaluated by the checkerboard method. Psychorubrin was active against Gram-positive bacteria, showing rapid time-dependent kinetics of bacterial killing, amplified nucleotide leakage, and greater activity against the methicillin-resistant species (MRSA) Staphylococcus aureus 33591 and 33592 and Staphylococcus pyogenes 10096. Psychorubrin also interfered with the composition of the biofilm matrix by reducing the total content of carbohydrates and proteins. A synergic effect between psychorubrin and chloramphenicol was observed for S. aureus 33592 and S. pyogenes 10096 while an additive effect was detected for S. aureus 33591. Our findings demonstrate, for the first time, an antagonistic activity of psychorubrin against bacteria not only in their planktonic forms but also in biofilms, and identify bacterial membranes as primary targets for this compound. Based on these observations, psychorubrin has a good potential for the design of novel antimicrobial agents.

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Antimicrobial Annona muricata L. (soursop) extract targets the cell membranes of Gram-positive and Gram-negative bacteria

Cited by 45 publications

References 39 publications

Moisture sorption isotherm and shelf‐life prediction of anticaking agent incorporated spray‐dried soursop (Annona muricata L.) powder

Moisture sorption isotherm and shelf‐life prediction of anticaking agent incorporated spray‐dried soursop (Annona muricata L.) powder

Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia

Antibacterial and Antibiofilm Activities of Psychorubrin, a Pyranonaphthoquinone Isolated From Mitracarpus frigidus (Rubiaceae)

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