Antimicrobial and toxicological evaluation of Origanum vulgare: an in vivo study

Abstract

Origanum vulgare has been of great interest in academia and pharma industry due to its antioxidant, antifungal and antitumor properties. The present study aimed to find the anti-MRSA potential and in vivo toxicity assessments of O. vulgare. O. vulgare extract was used to monitor anti-MRSA activity in mice. Following MRSA established infection in mice (Mus musculus), treatment with O. vulgare was continued for 7 days. Autopsies were performed and re-isolation, gross lesion scoring and bacterial load in various organs were measured. Additionally, blood sample was analysed for hematological assays. Toxicity assessment of O. vulgare potential as medicine was done at 200 mg/kg and 400 mg/kg by evaluating liver and kidney functions. Bacterial load and gross lesion in lungs and heart were significantly low compared to positive control following O. vulgare treatment. Likewise, O. vulgare treated groups had hematological, neutrophil and TLC values similar to control groups. Increased AST, ALP and total bilirubin alongwith marked hepatocellular degeneration and distortion around the central vein, inflammatory cell infiltration, and cytoplasmic vacuolization of hepatic cells was observed at higher dose. It is concluded that crude extract of O. vulgare may contain beneficial secondary metabolites and in future may be explored for curing infectious diseases.

Plant-Origin Components: New Players to Combat Antibiotic Resistance in Klebsiella pneumoniae

“…In vivo using Swiss albino mice In vivo acute oral toxicity testing did not show any toxic effects [115] Origanum vulgare In vivo using mice Continuous use or high doses may deliver undesirable components causing liver and renal function impairment [157] Thymus vulgaris In vivo using albino Holtzman rats While the 28-day oral toxicity test indicated that the no-observed-adverse-effect level (NOAEL) was more than 250 mg/kg/day, Thymus vulgaris had moderate oral toxicity.…”

Phytogenics in Ginger, Origanum vulgare, and Syzygium aromaticum and Their Potential as a Feed Additive against Clostridium perfringens in Broiler Production

“…A 90-day oral toxicity study of oregano essential oil in rats revealed that up to 200 mg/kg had no adverse effects on food/water consumption, body weight, hematology, biochemistry, organ weight, and histopathology [205]. A higher dose of Origanum vulgare extracts at 400 mg/kg, however, caused hepatotoxicity, including cellular degeneration and distortion, inflammatory infiltration, cytoplasmic vacuolization, sinusoidal congestion, and apoptosis in a 7-day study in mice [206].…”

Introduction of valuable medicinal plants of traditional medicine of Lamiaceae family in the conditions of the Tashkent Botanical Garden

“…(Hamidpour et al 2014;Zielińska et al 2014;Bozin et al 2017;Ivanov et al 2019;Mahmood et al 2020;Ghasempour et al 2022;Najafi et al 2022;Batiha et al 2023). Most species of the family Lamiaceae have been studied on chemical, pharmacological, ethnobotanic, clinical, ecological properties, etc., since they contain many different biologically active substances (Tahir et al 2018;Kompelly et al 2019;Soltani et al 2020;Tundis et al 2020;Liaqata et al 2023). The flora of Uzbekistan has 40 genera and 206 species of plants of the Lamiaceae family (Flora of Uzbekistan, 1961).…”