Antimicrobial and Larvicidal Activities of Stingless Bee Pollen from Maues, Amazonas, Brazil

Carneiro, Ana Lúcia Basílio; Gomes, André Alexandre; Silva, Lindair Alves da; Alves, Lincoln Basilio; Silva, Erveton Cardoso da; Pinto, Ana Cristina da Silva; Tadei, Wanderli Pedro; Pohlit, Adrian Martin; Teixeira, María Francisca Simas; Gomes, Candido Campos; Naiff, Maricleide de Farias

doi:10.1080/0005772x.2019.1650564

Cited by 8 publications

(7 citation statements)

References 37 publications

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“…Based on Tabel 1, revealed the % yeild of bee pollen extracts ranging 30.2–67.7%, and propolis extracts from 37.4 to 74.9%. These result are higher than reported by Carneiro et al (2019) regarding bee pollen extracts from stingless bee named M. compressipes manaosensis was 8.1–24.2% in 24 h extraction proces with ethanol and almost similar Pujirahayu et al, (2019) regarding propolis extracts from singles bee ( T. sapiens ) was 56.6 – 66.5% maserated in ethanol. In Fig.…”

Section: Discussioncontrasting

confidence: 57%

Cytotoxicity effect of honey, bee pollen, and propolis from seven stingless bees in some cancer cell lines

Arung

Ramadhan

Khairunnisa

et al. 2021

Saudi Journal of Biological Sciences

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Our effort to find new material for anti cancer from natural resources leads us to focus on stingless bee products such as honey, bee pollen, and propolis. The products were from seven stingless bees named Homotrigona fimbriata, Heterotrigona itama, Heterotrigona bakeri, Tetragonula sarawakensis, Tetragonula testaceitarsis, Tetragonula fuscobalteata, Tetragonula laeviceps. The stingless bee products were evaluated for their cytotoxicity effect on MCF-7, HeLa and Caco-2 cancer cell lines. This is the first time to be reported that the honey, ethanol extracts of bee pollen and propolis of H. fimbriata displayed more potent cytotoxicity than other stingless bee products. By chromatography and biological activity-guided fractionation, ethanol extract of propolis from H. fimbriata was fractionated and isolated its active compound named mangiferonic acid. Mangiferonic acid showed a cytotoxicity effect with IC 50 values 96.76 µM in MCF-7, >110.04 µM in HeLa, and > 110.04 µM in Caco-2, respectively. These results exhibited the potential of ethanol extracts from propolis of H. fimbriata to be further developed for drug and experiments to verify the function are essential.

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Section: Discussioncontrasting

confidence: 57%

Cytotoxicity effect of honey, bee pollen, and propolis from seven stingless bees in some cancer cell lines

Arung

Ramadhan

Khairunnisa

et al. 2021

Saudi Journal of Biological Sciences

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“…Many studies portray the difficulty in finding effective substances to inhibit the growth of gram-negative bacteria. Carneiro et al [ 32 ] evaluated the antimicrobial potential of pollen extract and propolis extract of M. compressipes manaosensis (jupará) in E. coli and did not obtain significant results in the analyses performed. Similarly, Tenorio et al [ 38 ] did not visualize the inhibiting action of Melipona fasciculata honey for E. coli and P. aeruginosa .…”

Section: Discussionmentioning

confidence: 99%

“…This study demonstrated a higher bactericidal effect of supernatants on methicillin-resistant S. aureus (MRSA) strains than against sensitive strains, different from that found in Gram-negative bacteria. Several studies have demonstrated a possible bactericidal action against MRSA from by-products of stingless bees or biomolecules produced by the associated microbiota [ 10 , 32 , 39 – 42 ]. Jenkins et al [ 43 ] found that the expression of MRSA genes decreased virulence due to exposure to different concentrations to Manuka honey and that, although the antimicrobial effect has it found, the mode of inhibition of quorum sensing of these bacterial cells have not yet it found, indicating the need for further studies.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial activity of supernatants produced by bacteria isolated from Brazilian stingless bee’s larval food

et al. 2022

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Background The discovery of new molecules with antimicrobial properties has been a promising approach, mainly when related to substances produced by bacteria. The use of substances produced by bees has evidenced the antimicrobial action in different types of organisms. Thus, the use of bacteria isolated from larval food of stingless bees opens the way for the identification of the new molecules. The effect of supernatants produced by these bacteria was evaluated for their ability to inhibit the growth of bacteria of clinical interest. Furthermore, their effects were evaluated when used in synergy with antibiotics available in the pharmaceutical industry. Results A few supernatants showed an inhibitory effect against susceptible and multiresistant strains in the PIC assay and the modulation assay. Emphasizing the inhibitory effect on multidrug-resistant strains, 7 showed an effect on multidrug-resistant Escherichia coli (APEC), Klebsiella pneumoniae carbapenemase (KPC), multidrug-resistant Pseudomonas aeruginosa, and multidrug-resistant Staphylococcus aureus (MRSA) in the PIC assay. Of the supernatants analyzed, some presented synergism for more than one species of multidrug-resistant bacteria. Nine had a synergistic effect with ampicillin on E. coli (APEC) or S. aureus (MRSA), 5 with penicillin G on E. coli (APEC) or KPC, and 3 with vancomycin on KPC. Conclusion In summary, the results indicate that supernatants produced from microorganisms can synthesize different classes of molecules with potent antibiotic activity against multiresistant bacteria. Thus, suggesting the use of these microorganisms for use clinical tests to isolate the molecules produced and their potential for use.

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“…Mohammad et al [113] revealed that Heterotrigona itama contains probiotic bacteria in stingless bee pollen for which its antimicrobial activity effectively inhibits foodborne pathogens. A study by Carneiro et al [117] on a Brazilian stingless bee, Melipona compres- Mohammad et al [113] revealed that Heterotrigona itama contains probiotic bacteria in stingless bee pollen for which its antimicrobial activity effectively inhibits foodborne pathogens. A study by Carneiro et al [117] on a Brazilian stingless bee, Melipona compressipes manaosensis, revealed that pollen produced by stingless bees contains active secondary metabolites that are a potential component for antibiotics and insecticides.…”

Section: Stingless Bee Pollenmentioning

confidence: 99%

A Comprehensive Review of Stingless Bee Products: Phytochemical Composition and Beneficial Properties of Honey, Propolis, and Pollen

et al. 2022

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The stingless bee has been gaining more attention in recent years due to the uniqueness and benefits of its products. Similar to the common honeybee, stingless bees also produce honey, propolis, and pollen, which offer superior benefits for direct or indirect consumption. However, reports on the benefits of stingless bee products are scarce. This article summarises recent reports on stingless bee products. The function and application of the properties of the products such as phenolic compounds, antioxidant properties, and chemical content are elucidated. The antimicrobial properties and anticancer potential of the products are also highlighted. Future trends, potential, and uniqueness of stingless bee products are discussed. Stingless bee honey is highlighted as a superfood that exceptionally has the potential to be an active ingredient in treating cancer. Stingless bee propolis has been extensively studied for its rich beneficial chemical compounds that contribute to its antioxidant properties. Though studies on stingless bee pollen are scarce, it has been reported that it also has the potential of being a functional food.

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Antimicrobial and Larvicidal Activities of Stingless Bee Pollen from Maues, Amazonas, Brazil

Cited by 8 publications

References 37 publications

Cytotoxicity effect of honey, bee pollen, and propolis from seven stingless bees in some cancer cell lines

Cytotoxicity effect of honey, bee pollen, and propolis from seven stingless bees in some cancer cell lines

Antimicrobial activity of supernatants produced by bacteria isolated from Brazilian stingless bee’s larval food

A Comprehensive Review of Stingless Bee Products: Phytochemical Composition and Beneficial Properties of Honey, Propolis, and Pollen

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