Antimicrobial and Immunomodulating Activities of Two Endemic Nepeta Species and Their Major Iridoids Isolated from Natural Sources

Aničić, Neda; Gašić, Uroš; Lu, Feng; Ćirić, Ana; Ivanov, Marija; Jevtić, Bojan; Dimitrijević, Mirjana; Anđelković, Boban; Škorić, Marijana; Živković, Jelena; Mao, Yingle; Liu, Jia; Tang, Chunping; Soković, Marina; Yang, Yong; Mišić, Danijela

doi:10.3390/ph14050414

Cited by 25 publications

(36 citation statements)

References 37 publications

(102 reference statements)

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“…Phenolics in the methanol extract from dried plant material ( Supplementary Table S2B ) were chromatographically separated and analyzed at TSQ Quantum Access Max QQQ mass spectrometer (ThermoFisher Scientific, Switzerland), as described by Aničić et al ( 2021 ). The Xcalibur software (version 2.2) was used for instrument control, data acquisition, and analysis.…”

Section: Methodsmentioning

confidence: 99%

“…Quantification of iridoids in the methanol extracts from dried plant material was performed in negative ion HESI mode of the UHPLC/qqqMS2 instrument, as described by Aničić et al ( 2021 ). Quantification of 1,5,9- e DLA acid was carried out using calibration curves of isolated standards, and the total amount of compounds in the samples was expressed as μg g −1 DW.…”

Section: Methodsmentioning

confidence: 99%

“…in Bory subsp. argolica , contain two nepetalactones and 1,5,9-epideoxyloganic acid (1,5,9- e DLA) as major iridoids (Aničić et al, 2021 ). The composition and importance of N. nuda phenolic compounds have been also previously reported, giving a special emphasis to phenolic acids, their derivatives, and flavonoids (Mišić et al, 2015 ; Aras et al, 2016b ; Fraga et al, 2017 ; Dienaite et al, 2018 ; Smiljković et al, 2018 ; Sarikurkcu et al, 2019 ; Hinkov et al, 2020 ; Aničić et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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Catmint (Nepeta nuda L.) Phylogenetics and Metabolic Responses in Variable Growth Conditions

et al. 2022

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Nepeta nuda (catmint; Lamiaceae) is a perennial medicinal plant with a wide geographic distribution in Europe and Asia. This study first characterized the taxonomic position of N. nuda using DNA barcoding technology. Since medicinal plants are rich in secondary metabolites contributing to their adaptive immune response, we explored the N. nuda metabolic adjustment operating under variable environments. Through comparative analysis of wild-grown and in vitro cultivated plants, we assessed the change in phenolic and iridoid compounds, and the associated immune activities. The wild-grown plants from different Bulgarian locations contained variable amounts of phenolic compounds manifested by a general increase in flowers, as compared to leaves, while a strong reduction was observed in the in vitro plants. A similar trend was noted for the antioxidant and anti-herpesvirus activity of the extracts. The antimicrobial potential, however, was very similar, regardless the growth conditions. Analysis of the N. nuda extracts led to identification of 63 compounds including phenolic acids and derivatives, flavonoids, and iridoids. Quantification of the content of 21 target compounds indicated their general reduction in the extracts from in vitro plants, and only the ferulic acid (FA) was specifically increased. Cultivation of in vitro plants under different light quality and intensity indicated that these variable light conditions altered the content of bioactive compounds, such as aesculin, FA, rosmarinic acid, cirsimaritin, naringenin, rutin, isoquercetin, epideoxyloganic acid, chlorogenic acid. Thus, this study generated novel information on the regulation of N. nuda productivity using light and other cultivation conditions, which could be exploited for biotechnological purposes.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Catmint (Nepeta nuda L.) Phylogenetics and Metabolic Responses in Variable Growth Conditions

et al. 2022

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“…A checkboard assay was used for the determination of interactions among the selected compounds and antibiotic, streptomycin. It was carried out with 96-well microplates containing TSB medium for the resistant P. aeruginosa strain, and supplemented with examined compounds in concentrations ranging from 1/16 to 4 × MIC as described previously [ 75 ] in the checkboard manner. The microplates were incubated for 24 h at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Thiazolidin-4-Ones as Potential Antimicrobial Agents: Experimental and In Silico Evaluation

et al. 2022

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Herein, we report computational and experimental evaluations of the antimicrobial activity of twenty one 2,3-diaryl-thiazolidin-4-ones. All synthesized compounds exhibited an antibacterial activity against six Gram-positive and Gram-negative bacteria to different extents. Thus, the MIC was in the range of 0.008–0.24 mg/mL, while the MBC was 0.0016–0.48 mg/mL. The most sensitive bacterium was S. Typhimurium, whereas S. aureus was the most resistant. The best antibacterial activity was observed for compound 5 (MIC at 0.008–0.06 mg/mL). The three most active compounds 5, 8, and 15, as well as compound 6, which were evaluated against three resistant strains, MRSA, P. aeruginosa, and E. coli, were more potent against all bacterial strains used than ampicillin. The antifungal activity of some compounds exceeded or were equipotent with those of the reference antifungal agents bifonazole and ketoconazole. The best activity was expressed by compound 5. All compounds exhibited moderate to good drug-likeness scores ranging from −0.39 to 0.39. The docking studies indicated a probable involvement of E.coli Mur B inhibition in the antibacterial action, while CYP51 inhibition is likely responsible for the antifungal activity of the tested compounds. Finally, the assessment of cellular cytotoxicity of the compounds in normal human MRC-5 cells revealed that the compounds were not toxic.

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“…A checkboard assay was used for the determination of interactions among the selected compounds and antibiotic and streptomycin. The assay was carried out with 96-well microplates containing TSB medium for the resistant P.aeruginosa strain, supplemented with examined compounds in concentrations ranging from 1/16 to 4 × MIC, as described previously, [81] in the checkboard manner. The microplates were incubated for 24 h at 37 • C. The MIC of the combinations of examined compounds with streptomycin was determined as for the antimicrobial assay.…”

Section: Checkboard Assaymentioning

confidence: 99%

4-(Indol-3-yl)thiazole-2-amines and 4-ιndol-3-yl)thiazole Acylamines as Νovel Antimicrobial Agents: Synthesis, In Silico and In Vitro Evaluation

Simakov

Карцев²,

Petrou

et al. 2021

Pharmaceuticals

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This manuscript deals with the synthesis and computational and experimental evaluation of the antimicrobial activity of twenty-nine 4-(indol-3-yl)thiazole-2-amines and 4-ιndol-3-yl)thiazole acylamines. An evaluation of antibacterial activity against Gram (+) and Gram (−) bacteria revealed that the MIC of indole derivatives is in the range of 0.06–1.88 mg/mL, while among fourteen methylindole derivatives, only six were active, with an MIC in the range of of 0.47–1.88 mg/mL. S. aureus appeared to be the most resistant strain, while S. Typhimurium was the most sensitive. Compound 5x was the most promising, with an MIC in the range of 0.06–0.12 mg/mL, followed by 5d and 5m. An evaluation of these three compounds against resistant strains, namely MRSA P. aeruginosa and E. coli, revealed that they were more potent against MRSA than ampicillin. Furthermore, compounds 5m and 5x were superior inhibitors of biofilm formation, compared to ampicillin and streptomycin, in terms Compounds 5d, 5m, and 5x interact with streptomycin in additive manner. The antifungal activity of some compounds exceeded or was equipotent to those of the reference antifungal agents bifonazole and ketoconazole. The most potent antifungal agent was found to be compound 5g. Drug likeness scores of compounds was in a range of −0.63 to 0.29, which is moderate to good. According to docking studies, E. coli MurB inhibition is probably responsible for the antibacterial activity of compounds, whereas CYP51 inhibition was implicated in antifungal activity. Compounds appeared to be non-toxic, according to the cytotoxicity assessment in MRC-5 cells.

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Antimicrobial and Immunomodulating Activities of Two Endemic Nepeta Species and Their Major Iridoids Isolated from Natural Sources

Cited by 25 publications

References 37 publications

Catmint (Nepeta nuda L.) Phylogenetics and Metabolic Responses in Variable Growth Conditions

Catmint (Nepeta nuda L.) Phylogenetics and Metabolic Responses in Variable Growth Conditions

Thiazolidin-4-Ones as Potential Antimicrobial Agents: Experimental and In Silico Evaluation

4-(Indol-3-yl)thiazole-2-amines and 4-ιndol-3-yl)thiazole Acylamines as Νovel Antimicrobial Agents: Synthesis, In Silico and In Vitro Evaluation

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