Antimicrobial and Environmental activity of biogenic CS-GO nanoparticles on Uropathogens

Abstract

Bioproduction options for nanoparticles are becoming a highly significant subject, with environmental and economic benefits over physical and chemical processes. The purpose of the current study is to detect the antimicrobial activity of chitosan-Graphene oxide nanoparticles that include The Minimum Inhibitory Concentration (MIC) is a method of determining antibacterial activity, Antibiofilm Activity by Tissue culture plate method, Antioxidant activity, and finally the hemolysis activity of mixing nanoparticles CS-GO that synthesis biologically. the result of Antibacterial activity showed the ID50 for inhibition is at a concentration (1 mg/ml), while the Antibiofilm activity result shows the high concentration of CS-GO nanoparticles (16 mg/ml) showed the higher activity in inhibition of biofilm formation while the less concentration (0.6mg/ml) appeared very low inhibition activity on biofilm formation. The antioxidant activity of nanoparticles showed the high scavenging ability at a concentration (600 mg/ml) as (54.06%) while the less concentration (100mg/ml) appeared scavenger ability as (29.42%) also the hemolysis activity on CS-GO nanoparticles on blood showed different ability at different concentration, the preferred concentration (1mg/ml) show no hemolysis on blood.

The potential of chitosan-silver nanoparticle-graphene oxide hybrid as an antimicrobial therapy against uropathogenic resistance in urinary tract infections

“…Biofilm inhibition is another critical feature of chitosan. A study by Alzubaidy et al 18 shows inhibition of E. coli biofilm. The anti-biofilm activity is due to the polycationic nature of chitosan, which interacts with EPS (extracellular polymeric substances), protein, and bacterial DNA, preventing biofilm production.…”

“…Chitosan combined with AgNP did not show significant hemolytic activity at MIC 42 . Chitosan combined with GO also did not significantly cause hemolysis at the median inhibitory dose (ID50) for E. coli 18 . Chitosan is also a sustainable resource, obtainable from crustacean shell waste.…”

“…The anti-biofilm activity is due to the polycationic nature of chitosan, which interacts with EPS (extracellular polymeric substances), protein, and bacterial DNA, preventing biofilm production. However, the study used chitosan linked with GO (Chit-GO) and did not evaluate the activity of chitosan by itself 18 . A study by Alfuraydi et al 15 evaluated the activity of pure chitosan against biofilm but found its inhibitory action to be poor, with a MIC as high as 1,000 µg/mL.…”