Antimicrobial and Cytotoxic Assessment of Marine Cyanobacteria - Synechocystis and Synechococcus

Martíns, Rosário; Ramos, Miguel F.; Herfindal, Lars; Sousa, José Pedro; Skærven, Kaja; Vasconcelos, Vítor

doi:10.3390/md6010001

Cited by 102 publications

(49 citation statements)

References 25 publications

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“…and Synechococcus spp. had antibacterial activity against Gram-positive bacteria, but not against Gram-negative ones (Martins et al 2008). Similarly, cyanobacteria isolated from benthic mats of Antarctic lakes have been shown to have antimicrobial activity (Biondi et al 2008).…”

Section: Cyanobacteriamentioning

confidence: 99%

Mini-review: Inhibition of biofouling by marine microorganisms

2013

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Any natural or artificial substratum exposed to seawater is quickly fouled by marine microorganisms and later by macrofouling species. Microfouling organisms on the surface of a substratum form heterogenic biofilms, which are composed of multiple species of heterotrophic bacteria, cyanobacteria, diatoms, protozoa and fungi. Biofilms on artificial structures create serious problems for industries worldwide, with effects including an increase in drag force and metal corrosion as well as a reduction in heat transfer efficiency. Additionally, microorganisms produce chemical compounds that may induce or inhibit settlement and growth of other fouling organisms. Since the last review by the first author on inhibition of biofouling by marine microbes in 2006, significant progress has been made in the field. Several antimicrobial, antialgal and antilarval compounds have been isolated from heterotrophic marine bacteria, cyanobacteria and fungi. Some of these compounds have multiple bioactivities. Microorganisms are able to disrupt biofilms by inhibition of bacterial signalling and production of enzymes that degrade bacterial signals and polymers. Epibiotic microorganisms associated with marine algae and invertebrates have a high antifouling (AF) potential, which can be used to solve biofouling problems in industry. However, more information about the production of AF compounds by marine microorganisms in situ and their mechanisms of action needs to be obtained. This review focuses on the AF activity of marine heterotrophic bacteria, cyanobacteria and fungi and covers publications from 2006 up to the end of 2012.

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Section: Cyanobacteriamentioning

confidence: 99%

Mini-review: Inhibition of biofouling by marine microorganisms

2013

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“…and R. gracilis. Martins et al (2008) reported inhibition of gram positive bacteria by extracts of marine Synechocystis and Synechococcus, and also observed the variations in the activity of different extracts.…”

Section: Discussionmentioning

confidence: 94%

“…Magalhães et al (2003) reported that S. aquatilis produced microcystin in Sepetiba Bay, Brazil, where were observed regular blooms of these cyanobacteria, and the microcystin was found in fish caught in this bay. Several works of Martins, Pereira, Welker, Fastner, and Vasconcelos (2005), Martins, Fernandez, Beiras, and Vasconcelos (2007) and Martins et al (2008) reported the production of microcystin by the marine cyanobacteria, including genus Synechocystis. Vareli et al (2012) reported the presence of the hepatotoxic microcystins in the Mediterranean Sea, and he suggested a potential association of microcystins with Synechococcus and/or Synechocystis cyanobacteria.…”

Section: Discussionmentioning

confidence: 99%

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Microcystins production and antibacterial activity of cyanobacterial strains of Synechocystis, Synechococcus and Romeria isolated from water and coral reef organisms of Brazilian coast

Barboza

Gorlach‐Lira

Sassi

et al. 2017

RBT

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Cyanobacteria are widely distributed in terrestrial, freshwater and marine environments, and over the past decades have been recognized as a powerful source of bioactive compounds. In this study, some cyanobacterial strains were isolated from samples of seawater, brackish water and tissue of reef benthic invertebrates (zoanthid Protopalythoa variabilis, the sponges Cynachrella sp. and Haliclona sp., the coral Siderastrea stellata, and ascidians), collected at the states of Paraíba and Rio Grande do Norte (Northeast of Brazil), during the period between July 2010 and February 2014. After standard isolation methods, the cultivation of the strains was carried out in acclimatized culture chamber (25 °C) under constant aeration, for 15 days at 12-hour photoperiod, using Conway and BG11 media made with filtered seawater. The cyanobacterial cells were analysed for the microcystin production by the ELISA technique and their ethanolic and methanolic extracts for the antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa by the agar well diffusion method. The detection of the mcyB gene, one of the genes related to the microcystin synthesis, was done by the Polymerase Chain Reaction (PCR) technique. The majority of the eighteen cyanobacterial strains belonged to Synechococcaceae Family. The genera of Synechocystis, Synechococcus and Romeria were represented by ten, six and two strains, respectively. The production of microcystins was observed in five strains belonging to the genus Synechocystis. The presence of mcyB gene was detected in 12 strains of cyanobacteria: Synechocystis (three strains), Synechococcus (six strains) and Romeria (two strains). Only one strain (Synechocystis aquatilis) showed both the microcystin production and the mcyB gene presence. The antibacterial activity was observed for one strain of Romeria gracilis, one strain of Synechocystis aquatilis and two strains of Synechococcus sp. The ethanolic extracts of R. gracilis strain and two Synechococcus spp. strains inhibited the growth of P. aeruginosa. Among methanolic extracts of cyanobacteria, only one strain of S. aquatilis showed activity against S. aureus, and one R. gracilis strain against P. aeruginosa. Some cyanobacterial strains studied were positive for the microcystin production and antibacterial activity against pathogenic bacteria S. aureus and P. aeuruginosa, and may be further explored for additional biotechnological applications. Rev. Biol. Trop. 65 (3): 890-899. Epub 2017 September 01.Key words: Synechococcales, extracts, mcyB, cyanobacteria.Cyanobacteria or blue-green algae are photosynthetic prokaryotes widely distributed in almost all habitats, from aquatic marine and freshwater ones to terrestrial environments, being also associated with various marine organisms such as corals and sponges (Glas et al., 2010;Paerl & Paul, 2011).In the last decades, cyanobacteria have been gaining attention in ecology, biochemistry, physiology and molecular biology, because of their high potential for antibiotics and ...

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“…One of the fascinating discoveries regarding biological activity is the anticancer property demonstrated among cyanobacterial genera such as Nostoc, Fhormidium, Gloeocapsa, Anabaena, Spirulina, Synechocystis and Synechococcus (Surakka et al 2005;Martins et al 2008;Gigova et al 2011;Oh et al 2011). Cyanobacteria produce a wide range of cytotoxic compounds, many of which are regarded as promising candidates for drug discovery, with applications in pharmacy, because of their natural origin, uniqueness and structural diversity (Singh et al 2005;Tan 2007;Gademann and Portmann 2008).…”

Section: Introductionmentioning

confidence: 99%